Goat anti rabbit horseradish peroxidase conjugated iggs

Anti-human CYP2J2 antibodies used for immunoblot analysis were prepared as described previously (King et al. 2002 (link)). Anti-rat CYP4A antibodies and anti-human CYP4F11 antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-human CYP4F3 antibodies were purchased from Abnova (Taipei, Taiwan). Anti-human CYP4F12 antibodies were purchased from Abcam (Cambridge, MA). The secondary antibodies (goat anti-mouse, donkey anti-goat, and goat anti-rabbit horseradish peroxidase-conjugated IgGs) were purchased from Santa Cruz Biotechnology. Polyvinylidene difluoride (PVDF) membranes and reagents for enhanced chemiluminescence were purchased from GE Healthcare (Piscataway, NJ). [1-¹⁴C] Lauric acid was purchased from American Radiolabeled Chemicals (St. Louis, MO). All other chemicals and reagents were of electrophoresis or analytical grade where appropriate.

Polyclonal antibodies used in this study were commercially available; anti-human CYP1A1 antibody from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA), anti-human CYP2A6, anti-human CYP2C9, anti-human CYP2D6, and anti-human CYP3A4 antibodies from Nosan Corporation (Yokohama, Japan), anti-human CYP2B6 and anti-human CYP3A5 antibodies from BD Gentest (Woburn, MA), and anti-human CYP2E1 antibody from BIOMOL Research Laboratories (Plymouth Meeting, PA). Anti- cynomolgus CYP2C76 and anti-human CYP2J2 antibodies were prepared as described previously (King et al. 2002 (link); Uno et al. 2006 (link); Uno et al. 2011b (link)). The secondary antibodies (goat anti-mouse, donkey anti-goat, and goat anti-rabbit horseradish peroxidase-conjugated IgGs) were purchased from Santa Cruz Biotechnology, Inc. Chemicals and reagents for the polyacrylamide gels, including sodium dodecyl sulfate (SDS), bis/acrylamide (37.5:1), ammonium persulfate, and TEMED were purchased from Bio-Rad Laboratories (Hercules, CA). Polyvinylidene difluoride membranes (Hybond-P) and an enhanced chemiluminescence Western blotting detection reagent were purchased from GE Healthcare (Chalfont St. Giles, Buckinghamshire, UK). All other chemicals and reagents were purchased from Sigma (St. Louis, MO) or Wako Pure Chemical Industries (Osaka, Japan) unless otherwise specified.