The chemical compositions of the EOs of C. goetheanus (A, B, and C), were analyzed using a Shimadzu QP-2010 plus (Kyoto, Japan) a gas chromatography system equipped with an Rtx-5MS capillary column (30 m × 0.25 mm; 0.25 µm film thickness) (Restek Corporation, Bellefonte, PA, USA) coupled to a mass spectrometer (GC/MS) (Shimadzu, Kyoto, Japan). The program temperature was maintained at 60–240 °C at a rate of 3 °C/min, with an injector temperature of 250 °C, helium as the carrier gas (linear velocity of 32 cm/s, measured at 100 °C), and a splitless injection (1 μL of a 2:1000 hexane solution), using the same operating conditions as described in the literature [6 (link),88 (link),89 (link),90 (link)]). The components were quantified using gas chromatography (GC) on a Shimadzu QP-2010 system (Kyoto, Japan), equipped with a flame ionization detector (FID) (Kyoto, Japan), under the same operating conditions as before, except for the carrier hydrogen gas. The retention index for all volatile constituents was calculated using a homologous series of n-alkanes (C8–C40) Sigma-Aldrich (St. Louis, MI, USA), according with Van den Dool and Kratz [91 (link)]. The components were identified by comparison (i) of the experimental mass spectra with those compiled in libraries (reference) and (ii) their retention indices to those found in the literature [32 ,33 ].
Qp 2010 system
Manufactured by Shimadzu
Sourced in Japan
The QP-2010 system is a gas chromatograph-mass spectrometer (GC-MS) designed for analytical applications. It combines gas chromatography for sample separation with mass spectrometry for compound identification and quantification. The system is capable of performing a wide range of analytical tasks, including environmental analysis, food safety, and forensic investigations.
Automatically generated - may contain errors
Lab products found in correlation
N alkanes c8 c40, by Merck Group (6 mentions)
Gc ms, by Shimadzu (5 mentions)
Rtx 5ms capillary column, by Restek (5 mentions)
Qp2010, by Shimadzu (4 mentions)
Qp 2010 plus system, by Shimadzu (3 mentions)
Qp2010 plus, by Shimadzu (3 mentions)
Rtx 5ms, by Shimadzu (2 mentions)
Gcmssolution software, by Shimadzu (1 mentions)
N alkanes c8 c24, by Merck Group (1 mentions)
Db 5ms column, by Shimadzu (1 mentions)
Db 5ms, by Shimadzu (1 mentions)
Gc2010a, by Shimadzu (1 mentions)
Pentane, by Merck Group (1 mentions)
Gc 2010, by Shimadzu (1 mentions)
Zebron zb 5 capillary column, by Phenomenex (1 mentions)
Db 5ms, by Agilent Technologies (1 mentions)
Gc 2014, by Shimadzu (1 mentions)
Hp 5ms, by Hewlett-Packard (1 mentions)
19 protocols using qp 2010 system
1
GC-MS Analysis of C. goetheanus EOs
2
GC-MS Analysis of Essential Oil Composition
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The chemical composition of the EOs was evaluated according to a reported methodology [51 (link)], by using gas chromatography/mass spectrometry (Shimadzu, QP-2010 plus system, (City Kyoto, Japan), under the following conditions: silica capillary column Rtx-5MS (30 m × 0.25 mm, film thickness = 0.25 μm), program temperature of 60–240 °C (3 °C/min), injector temperature of 250 °C, helium as drag gas (linear velocity of 32 cm/s, measured at 100 °C), and splitless injection (1 μL of a 2:1000 hexane solution). Ionization was obtained by the electronic impact technique at 70 eV; the temperature of the ion source and other parts was 200 °C. The volatile compounds were quantified by gas chromatography using a flame ionization detector (FID) (Shimadzu, QP 2010 system), under the same conditions as GC/MS, except that nitrogen was used as the drag gas. The retention index was calculated for all the volatile constituents using a homologous series of n-alkanes (C8–C20). They were identified by comparison of their mass spectra and retention indices to those reported in the literature [22 ,52 ].
3
Chemical Composition Analysis of Basil Essential Oil
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The chemical compositions of the EO of O. basilicum var. minimum were analyzed as reported by our research group [52 (link),53 (link)], using a Shimadzu QP-2010 (Kyoto, Japan) plus gas chromatography system equipped with an Rtx-5MS capillary column (Restek Corporation, Bellefonte, PA, USA) (30 m × 0.25 mm; 0.25 µm film thickness) coupled with a mass spectrometer (GC/MS) (Shimadzu, Kyoto, Japan) and the components were quantified using gas chromatography (CG) on a Shimadzu QP-2010 system (Kyoto, Japan), equipped with a flame ionization detector (FID). The program temperature and injection were the same operating conditions as described in the literature [54 (link),55 ], except for the carrier hydrogen gas, under the same operating conditions as before. The retention index for all volatile constituents was calculated using a homologous series of n-alkanes (C8–C40) Sigma-Aldrich (San Luis, CA, USA), according to van den Dool and Kratz [56 (link)]. The components were identified by comparison of: (i) the experimental mass spectra with those compiled in libraries, and (ii) their retention indices to those found in the literature [57 ].
4
GC/MS Analysis of Essential Oil Composition
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The chemical composition of the essential oils was evaluated by gas chromatography/mass spectrometry (GC/MS) according to the methodologies by [53 (link),54 (link)], using a Shimadzu QP-2010 plus system under the following conditions: silica capillary column Rtx-5MS (30 m × 0.25 mm, 0.25 μm film thickness); program temperature of 60–240 °C at 3 °C/min; injector temperature of 250 °C; helium as carrier gas (linear velocity of 32 cm/s, measured at 100 °C); splitless injection (1 μL of a 2:1000 hexane solution). Ionization was obtained by electronic impact technique at 70 eV, and the temperature of the ion source and other parts was set at 200 °C. The quantification of volatile compounds was determined by gas chromatography with a flame ionization detector (FID; Shimadzu, QP 2010 system-Kyoto, Japan) under the same conditions as gas chromatography coupled to mass spectrometry (GC-MS), except that hydrogen was used as the carrier gas. The retention index was calculated for all volatile constituents using a homologous series of n-alkanes (C8–C20), and were identified by comparing the mass spectra obtained experimentally and their retention indices to those found in literature [46 ,47 ].
5
Chemical Profiling of Essential Oils
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The chemical compositions of the EOs of M. multiflora (A, B and C) and E. florida, were analyzed using a Shimadzu QP-2010 plus (Kyoto, Japan), a gas chromatography system equipped with an Rtx-5MS capillary column (30 m × 0.25 mm; 0.25 µm film thickness) (Restek Corporation, Bellefonte, PA, USA) coupled to a mass spectrometer (GC/MS) (Shimadzu, Kyoto, Japan). The programmed temperature was maintained at 60–240 °C at a rate of 3 °C/min, with an injector temperature of 250 °C, helium as the carrier gas (linear velocity of 32 cm/s, measured at 100 °C) and a splitless injection (1 μL of a 2:1000 hexane solution), using the same operating conditions as described in the literature [62 (link)]. Except for the carrier hydrogen gas, the components were quantified using gas chromatography (CG) on a Shimadzu QP-2010 system (Kyoto, Japan), equipped with a flame ionization detector (FID) (Kyoto, Japan), under the same operating conditions as before. The retention index for all volatile constituents was calculated using a homologous series of n-alkanes (C8–C40), Sigma-Aldrich (San Luis, MO, USA), according to van den Dool and Kratz [63 (link)]. The components were identified by comparison (i) of the experimental mass spectra with those compiled in libraries (reference) and (ii) their retention indices to those found in the literature [38 ,39 ].
6
Quantification of Cuticular Hydrocarbon Profiles
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Samples for were prepared by incubating five to eight of each genotype at room temperature for 20 min with 120 µl of hexane containing 10 µg/ml hexacosane as an internal standard. The extract (100 µl) was transferred into a fresh glass vial and allowed to evaporate at room temperature. Samples were stored at –20°C. Three replicates were prepared for each genotype.
Analysis by gas chromatography mass spectrometry (GCMS) was performed on a QP2010 system (Shimadzu, Kyoto, Japan) equipped with a DB-5 column (5%-phenyl-methylpolysiloxane column; 30 m length, 0.25 mm ID, 0.25 μm film thickness; Agilent). Ionization was achieved by electron ionization (EI) at 70 eV. One microliter of the sample was injected using a splitless injector. The helium flow was set at 1.9 ml/min. The column temperature program began at 50°C, increased to 210°C at a rate of 35°C/min, and then increased to 280°C at a rate of 3°C/min. A mass spectrometer was set to unit mass resolution and three scans/s from m/z 37 to 700. Chromatograms and mass spectra were analyzed using GCMSsolution software (Shimadzu). For total CHC levels, the area under each of the CHC peaks were summed and normalized to the area under the peak for the spiked hexacosane standard. Statistical analysis was performed using a one-way ANOVA with post-hoc Holm-Sidak test.
Analysis by gas chromatography mass spectrometry (GCMS) was performed on a QP2010 system (Shimadzu, Kyoto, Japan) equipped with a DB-5 column (5%-phenyl-methylpolysiloxane column; 30 m length, 0.25 mm ID, 0.25 μm film thickness; Agilent). Ionization was achieved by electron ionization (EI) at 70 eV. One microliter of the sample was injected using a splitless injector. The helium flow was set at 1.9 ml/min. The column temperature program began at 50°C, increased to 210°C at a rate of 35°C/min, and then increased to 280°C at a rate of 3°C/min. A mass spectrometer was set to unit mass resolution and three scans/s from m/z 37 to 700. Chromatograms and mass spectra were analyzed using GCMSsolution software (Shimadzu). For total CHC levels, the area under each of the CHC peaks were summed and normalized to the area under the peak for the spiked hexacosane standard. Statistical analysis was performed using a one-way ANOVA with post-hoc Holm-Sidak test.
7
Bacterial Fatty Acid Profiling
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The FA methyl esters (FAMEs) were extracted using the standardized MIDI protocol (http://www.microbialid.com/PDF/TechNote_101.pdf ). Bacterial cells were obtained from culturing at 30°C (at or close to optimal conditions for all seven phylogenetic groups) on trypticase soy broth agar (TSBA, 30 g trypticase soy broth and 15 g Bacto agar; l−1) for 24 h as previously described [21 (link)]. After extraction, FAMEs were analyzed by gas chromatography-mass spectrometry (GC–MS) (Shimadzu QP2010 system), as previously described [27 (link)].
8
Volatile Compounds Analysis of Cocoa Beans
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The fermented and dried cocoa beans were milled (A11B, Ika) and subjected to simultaneous distillation/extraction for 2 hours using 4 mL of pentane as a solvent. The volatile concentrate obtained was stored at a temperature of 5 °C [52 (link),53 (link),54 (link)].
Aliquots (2 μL) of volatile concentrate was injected into a gas chromatograph coupled to mass spectrometry (GC-MS), in a Shimadzu QP-2010 Plus system, equipped with a DB-5MS column (30 m × 0.25 mm × 0.25 μm). The helium gas was used as the carrier gas with a flow rate of 1.2 mL/min. The temperature of the injector and the interface was 250 °C, and the oven temperature was adjusted to 60–250 °C, using a ramp of 3 °C/min. Electronic impact mass spectrometer at 70 eV and the ion source temperature at 220 °C were used.
Quantitative analysis of the chemical constituents was performed by peak-area normalization using a flame ionization detector (FID—Shimadzu, QP 2010 system) under the same conditions as GC-MS, except that hydrogen was used as a mobile phase. Chemical identification was carried out by comparing the mass spectra and retention indices (RI) with those of standard substances in the system libraries and with data from the literature [55 ,56 ,57 ]. The RIs were obtained using a homologous series of n-alkanes (C8–C24, Sigma-Aldrich Co, St. Louis, MO, USA).
Aliquots (2 μL) of volatile concentrate was injected into a gas chromatograph coupled to mass spectrometry (GC-MS), in a Shimadzu QP-2010 Plus system, equipped with a DB-5MS column (30 m × 0.25 mm × 0.25 μm). The helium gas was used as the carrier gas with a flow rate of 1.2 mL/min. The temperature of the injector and the interface was 250 °C, and the oven temperature was adjusted to 60–250 °C, using a ramp of 3 °C/min. Electronic impact mass spectrometer at 70 eV and the ion source temperature at 220 °C were used.
Quantitative analysis of the chemical constituents was performed by peak-area normalization using a flame ionization detector (FID—Shimadzu, QP 2010 system) under the same conditions as GC-MS, except that hydrogen was used as a mobile phase. Chemical identification was carried out by comparing the mass spectra and retention indices (RI) with those of standard substances in the system libraries and with data from the literature [55 ,56 ,57 ]. The RIs were obtained using a homologous series of n-alkanes (C8–C24, Sigma-Aldrich Co, St. Louis, MO, USA).
9
GC-MS Analysis of Eucalyptus florida Essential Oils
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The chemical compositions of the EOs of E. florida (A and B), were analyzed using a Shimadzu QP-2010 plus (Kyoto, Japan) a gas chromatography system equipped with an Rtx-5MS capillary column (30 m × 0.25 mm; 0.25 µm film thickness) (Restek Corporation, Bellefonte, PA, USA) coupled to a mass spectrometer (GC/MS) (Shimadzu, Kyoto, Japan). The program temperature was maintained at 60–240 °C at a rate of 3 °C/min, with an injector temperature of 250 °C, helium as the carrier gas (linear velocity of 32 cm/s, measured at 100 °C) and a splitless injection (1 μL of a 2:1000 hexane solution), using the same operating conditions as described in the literature [67 (link),68 (link)]. Except for the carrier hydrogen gas, the components were quantified using gas chromatography (CG) on a Shimadzu QP-2010 system (Kyoto, Japan), equipped with a flame ionization detector (FID) (Kyoto, Japan), under the same operating conditions as before. The retention index for all volatile constituents was calculated using a homologous series of n-alkanes (C8–C40) Sigma-Aldrich (San Luis, USA), according with Van den Dool and Kratz [69 (link)]. The components were identified by comparison (i) of the experimental mass spectra with those compiled in libraries (reference) and (ii) their retention indices to those found in the literature [28 ,70 ,71 ].
10
Gas Chromatography-Mass Spectrometry Analysis
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Gas chromatography–mass spectrometric analyses were performed on a QP2010 system (Shimadzu, Kyoto, Japan) by electronic ionization at 70 eV, employing a chromatographic column (DB-5MS, 30 mm × 0.25 mm, 0.25 μm). Each extract (1 μL) was injected using the splitless injection method. The column temperature was increased from 60 °C to 200 °C at the rate of 6 °C/min (held at 200 °C for 6 minutes), then raised to 220 °C by 5 °C/min, next to 250 °C by 4 °C/min, and finally held at 250 °C for 5 minutes. The injection port was set at 250 °C. Helium gas was used as the carrier at a flow rate of 1 mL/min.
Relative percentage data were obtained from electronic integration of peak areas without the use of a correction factor. The software adopted to handle mass spectra and chromatograms was GC-MS Solutions. Retention indices were determined using retention times of normal alkanes that had been injected after the oil under the same chromatographic conditions mentioned above. The compounds were identified by comparison of their mass spectra with the NIST05.LIB database or with published mass spectra.
Relative percentage data were obtained from electronic integration of peak areas without the use of a correction factor. The software adopted to handle mass spectra and chromatograms was GC-MS Solutions. Retention indices were determined using retention times of normal alkanes that had been injected after the oil under the same chromatographic conditions mentioned above. The compounds were identified by comparison of their mass spectra with the NIST05.LIB database or with published mass spectra.
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