Trizol plus rna isolation reagents

Manufactured by Thermo Fisher Scientific

Sourced in United States

TRIzol Plus RNA Isolation Reagents are a set of reagents designed for the isolation and purification of total RNA from various biological samples. The core function of these reagents is to facilitate the extraction and separation of RNA from DNA, proteins, and other cellular components.

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Lab products found in correlation

Reverse transcription kit, by Takara Bio (2 mentions) Abi 7500ht fast real time pcr system, by Thermo Fisher Scientific (2 mentions) Taqman mirna assay kit, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

TRIzol reagent (85338 citations) TRIzol RNA isolation reagent (391 citations) TRIzol RNA reagent (88 citations) TRIzol isolation reagent (42 citations) RNA Trizol (35 citations) TRIzol Plus (20 citations) TRIzol isolation (13 citations) Trizol RNA isolation (7 citations) RNA Isolation Reagent (5 citations) Trizol Plus reagent (2 citations)

3 protocols using trizol plus rna isolation reagents

Real-Time Quantification of miRNA and mRNA

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Total RNA was extracted from tissues and cells by using TRIzol™ Plus RNA Isolation Reagents (Invitrogen). The reverse transcription kit (Takara, Otsu, Japan) was applied for RNAs reverse transcription. qRT-PCR was performed on ABI 7500HT Fast Real-Time PCR System (Applied Biosystems, CA, USA) under the following reaction conditions: 95 °C for 3 min, 40 cycles at 95 °C for 15 s, 60 °C for 30 s, and 72 °C for 20 s. The mRNA expression level was calculated using the

2^{- Δ Δ C_{t}}

method. The primer sequences are shown in Table 1. U6 or β–actin was used as the internal reference of miR-125b or SIRT7, respectively.Table 1

Primer sequences

Name of primer	Sequences
miR-125b	Forward: 5′-GAATCCCTGAGACCCTAAC-3′
miR-125b	Reverse: 5′-GTGCAGGGTCCGAGGT-3′
U6	Forward: 5′-CTCGCTTCGGCAGCACA-3′ Reverse: 5′-AACGCTTCACGAATTTGCGT-3′
SIRT7	Forward: 5′-TCTCTGAGCTCCATGGGAAT-3′
SIRT7	Reverse: 5′-CATGAGGAGCCGCATTACAT-3′
β-Actin	Forward: 5′-ACACCTTCTACAATGAGCTG-3′
β-Actin	Reverse: 5′-CTGCTTGCTGATCCACATCT-3′

Chen Q., Liu Y., Ding X., Li Q., Qiu F., Wang M., Shen Z., Zheng H, & Fu G. (2019). Bone marrow mesenchymal stem cell-secreted exosomes carrying microRNA-125b protect against myocardial ischemia reperfusion injury via targeting SIRT7. Molecular and Cellular Biochemistry, 465(1), 103-114.

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Quantitative Analysis of miR-96-5p and SFRP4

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TRIzol Plus RNA Isolation Reagents (Invitrogen) was utilized to isolate RNA from CC tissues and cells. RNAs were reverse transcribed using the reverse transcription kit (Takara). Quantitative real-time polymerase chain reaction (qRT-PCR) was conducted using the miRNA-specific TaqMan MiRNA Assay Kit (Applied Biosystems) on ABI 7500HT Fast Real-Time PCR System (Applied Biosystems). The PCR amplification conditions were 95 °C for 3 minutes, 40 cycles at 95 °C for 15 seconds, 60 °C for 30 seconds, and 72 °C for 1 minute. The mRNA expression level was calculated using the 2⁻^ΔΔ^Ct method. U6 or GAPDH was used as the internal reference of miR-96-5p or SFRP4. The primer sequences are listed in Table 1.

Zhang H., Chen R, & Shao J. (2020). MicroRNA-96-5p Facilitates the Viability, Migration, and Invasion and Suppresses the Apoptosis of Cervical Cancer Cells byNegatively Modulating SFRP4. Technology in Cancer Research & Treatment, 19, 1533033820934132.

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Quantifying RNA Expression Levels

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Total RNA was prepared by TRIzol Plus RNA Isolation Reagents (Invitrogen, Carlsbad, CA, USA). The RNA was analyzed using 5× All-In-One RT MasterMix (Applied Biological Material, Richmond, BC, Canada) and BlasTaq™ 2× qPCR MasterMix (Applied Biological Material). Housekeeping genes actin and U6 were internal controls for expression of NKILA, ELAVL1 and miR-205-5p. RNA expression levels were overall evaluated by comparative Cycle Threshold (Ct) values using 2 -ΔΔCt formula. For RT-qPCR analysis, primers were shown in Table 1.

Zhang Y, & Chen C. (2023). Knockdown of lncRNA NKILA suppresses sevoflurane-induced neuronal cell injury partially by targeting miR-205-5p/ELAVL1 axis. General physiology and biophysics, 42(3).

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