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Enzyme linked immunosorbent assay elisa kits

Manufactured by BD
Sourced in United States

Enzyme-linked immunosorbent assay (ELISA) kits are a type of analytical biochemistry assay used to detect and quantify specific substances, typically proteins, antibodies, or hormones, in a liquid sample. The kits utilize antibodies and color change to measure the concentration of the target analyte in the sample.

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2 protocols using enzyme linked immunosorbent assay elisa kits

1

Investigating the Effects of LPS and UDCA

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LPS (L-2880-10 mg) was obtained from Sigma Chemical Co. (St. Louis, MO). This was supplied as a lyophilized powder for reconstitution as a 5 ml sterile saline solution (NaCl 0.9%). UDCA was provided in suspension form by Ursofalk (Dr Falk Pharma GmbH, in Freiburg, Germany). Aspartate aminotransferase (AST), alkaline phosphatase (ALP), alanine aminotransferase (ALT), and total bilirubin (TBIL) kits were purchased from Wako Pure Chemical Industries (Osaka, Japan). Annexin V-FITC apoptosis detection kits were purchased from Aldrich Chem. Corp. (USA). TNF-alpha kits and interleukin kits (IL1, IL4) were obtained from the ASSAYPRO Co. (Universal Biologicals Ltd., Cambridge, UK). Enzyme-linked immunosorbent assay (ELISA) kits and T/B/NK kits were purchased from BD Biosciences Co. (Franklin Lakes, New Jersey, US).
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2

DNCB-induced Atopic Dermatitis Model

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Eight-week-old female BALB/c mice were purchased from the Guangdong Animal Centre (Guangzhou, China) and housed under specific pathogen-free conditions at 22°C ± 2°C, 45% ± 10% relative humidity, and a 12 h light/dark cycle. Furthermore, 2,4-dinitrochlorobenzene (DNCB) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Acetone and olive oil were obtained from Aladdin (Shanghai, China). Enzyme-linked immunosorbent assay (ELISA) kits were purchased from BD Biosciences (San Jose, CA, USA). The TRIzol reagent was purchased from Thermo Fisher Scientific (Waltham, MA, USA).
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