β eudesmol

Manufactured by Fujifilm

Sourced in Japan

β-eudesmol is a naturally occurring sesquiterpenoid compound. It is a constituent of various essential oils and is commonly used in laboratory settings for research and analytical purposes. The core function of β-eudesmol is to serve as a reference standard and chemical marker for the identification and quantification of natural products and their derivatives.

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Lab products found in correlation

Thioperamide, by Merck Group (1 mentions) Carboxymethylcellulose, by Merck Group (1 mentions) Rpmi 1640 medium, by Fujifilm (1 mentions) Cdcl3, by Merck Group (1 mentions) Cd3od d4, by Merck Group (1 mentions)

5 protocols using β eudesmol

Zebrafish Embryo Exposure to Bioactive Compounds

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β-Eudesmol and atractylodin, in their pure form (99.99%), were bought from Wako
Pure Chemical Industries Ltd (Osaka, Japan). Solvents used for dissolving
β-Eudesmol and atractylodin were ethanol (EtOH) and dimethyl sulfoxide (DMSO),
respectively. Preparation of different concentrations of the test compounds was
done by diluting the stock solutions in embryo medium (E3). The highest
concentrations of EtOH and DMSO in the final test solutions were 1.11% and 0.6%,
respectively, not exceeding the lowest permitted concentrations that cause
phenotypic and molecular level defects in the zebrafish embryos.^{9 –11 (link, link)}

Tshering G., Pimtong W., Plengsuriyakarn T, & Na-Bangchang K. (2022). Effects of β-eudesmol and atractylodin on target genes and hormone related to cardiotoxicity, hepatotoxicity, and endocrine disruption in developing zebrafish embryos. Science Progress, 105(4), 00368504221137458.

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Rat TRPA1 Channel Expression

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β-Eudesmol was purchased from Wako Pure Chemical Industries (Osaka, Japan). Thioperamide and carboxymethylcellulose were purchased from Sigma-Aldrich (MO, USA) and Calbiochem (MA, USA). Rat TRPA1 channel expression vector was purchased from OriGene Technologies (MD, USA).

Ohara K., Fukuda T., Ishida Y., Takahashi C., Ohya R., Katayama M., Uchida K., Tominaga M, & Nagai K. (2017). β-Eudesmol, an oxygenized sesquiterpene, stimulates appetite via TRPA1 and the autonomic nervous system. Scientific Reports, 7, 15785.

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Authentication of Atractylodes Rhizome Samples

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An Atractylodes rhizome sample collected from Shaanxi Province, China was purchased from Tochimoto Tenkaido Co. Ltd and authenticated as Sojutsu by Dr. Yutaka Yamamoto (Tochimoto Tenkaido Co. Ltd., Osaka, Japan). This sample consisted of small cut-up pieces of rhizome. Whole-plant specimens of A. lancea, A. chinensis, and A. japonica were collected from the Herb Garden of Yokohama University of Pharmacy (Yokohama, Japan) and authenticated. These voucher samples were deposited in the Ritsumeikan Herbarium of Pharmacognosy, Ritsumeikan University, under code numbers RIN-AC-020 (Sojutsu sample), AC-021 (A. chinensis), AL-022 (A. lancea), and AJ-023 (A. japonica). As standards, β-eudesmol and (−)-α-bisabolol were purchased from FUJIFILM Wako Pure Chemical Corporation and Sigma-Aldrich Corp. (St. Louis, MO, USA), respectively.

Ishii T., Okuyama T., Noguchi N., Nishidono Y., Okumura T., Kaibori M., Tanaka K., Terabayashi S., Ikeya Y, & Nishizawa M. (2019). Antiinflammatory constituents of Atractylodes chinensis rhizome improve glomerular lesions in immunoglobulin A nephropathy model mice. Journal of Natural Medicines, 74(1), 51-64.

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CCA Cell Lines Maintenance Protocol

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HuH28 (derived from a 37 years old female) and HuCCT1 (derived from a 56 years old male) CCA cell lines were maintained at Chulabhorn International College of Medicine, Thammasat University, Thailand. Cells were propagated and cultured at 37°C in a humidified atmosphere (5% CO₂) using RPMI-1640 medium (Wako, Osaka, Japan) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. Primary antibodies were purchased from Cell Signaling Technology, MA, USA (caspase3 #9665 and caspase-8 #4790), Santa Cruz Biotechnology, Inc., TX, USA (p21 #sc-6246, caspase-9 #sc-17784 and Bax #sc-20067), and Medical and Biological Laboratories Co., Nagoya, Japan (anti-β-actin pAB-HRP-DirectT). Secondary antibodies used were from Promega, WI, USA (anti-rabit IgG HRP) and Santa Cruz biotechnology, Inc., (anti-mouse-IgGk BP-HRP). β-eudesmol was purchased from Wako [Figure S1].

Narahara C., Saeheng T., Chaijaroenkul W., Dumre S.P., Na-Bangchang K, & Karbwang J. (2020). β-Eudesmol induces the expression of apoptosis pathway proteins in cholangiocarcinoma cell lines. Journal of Research in Medical Sciences : The Official Journal of Isfahan University of Medical Sciences, 25, 7.

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Atractylodes Metabolite Identification

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Atractylodes standards, β-eudesmol, and atractylenolide III were obtained from Fujifilm Wako Pure Chemical Co. (Osaka, Japan). For NMR acquisition, CD₃OD-d₄ (99.8% D) and CDCl₃ (99.8% D) were purchased from Merck KGaA (Darmstadt, Germany). The signals in the ¹H NMR spectra of Atractylodes extracts were assigned to individual metabolites on the basis of thorough analyses of the 2D NMR spectra and spiking experiments. The PCR-grade tubes, tips, and most biological reagents used for DNA authentication were acquired from Qiagen (Valencia, CA, USA) and/or Thermo-Fisher Scientific and Beckman Coulter (Indianapolis, IN, USA). LO3 agarose for gel electrophoresis was purchased from Takara (Kyoto, Japan).

Shirahata T., Ishikawa H., Kudo T., Takada Y., Hoshino A., Taga Y., Minakuchi Y., Hasegawa T., Horiguchi R., Hirayama T., Konishi T., Takemoto H., Sato N., Aragane M., Oikawa T., Odaguchi H., Hanawa T., Kodaira E., Fukuda T, & Kobayashi Y. (2021). Metabolic fingerprinting for discrimination of DNA-authenticated Atractylodes plants using 1H NMR spectroscopy. Journal of Natural Medicines, 75(3), 475-488.

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