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Anti ogdh

Manufactured by Cell Signaling Technology
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Anti-OGDH is a highly specific antibody that recognizes the OGDH (2-Oxoglutarate Dehydrogenase) protein. OGDH is a key enzyme in the tricarboxylic acid (TCA) cycle, also known as the Krebs cycle, which is a central metabolic pathway in cellular respiration. The Anti-OGDH antibody is a valuable tool for researchers studying OGDH and its role in cellular metabolism and energy production.

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Lab products found in correlation

Horseradish peroxidase linked secondary antibody, by Cell Signaling Technology (1 mentions) Ecl chemiluminescence detection system, by Thermo Fisher Scientific (1 mentions) Anti β actin, by Santa Cruz Biotechnology (1 mentions) Anti pyruvate dehydrogenase, by Cell Signaling Technology (1 mentions) Prolong gold antifade with dapi, by Thermo Fisher Scientific (1 mentions) Anti ogdh, by Merck Group (1 mentions) Anti acaa2, by OriGene (1 mentions)

2 protocols using anti ogdh

1

Investigating Metabolic Responses to CPI-613 and Radiation

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Cells (1 × 106 MiaPaCa-2) were grown in 100-mm Petri dishes overnight. The following day, cells were treated with either CPI-613 alone, radiation alone, or both for 24 hours. Cells were lysed in radioimmunoprecipitation assay buffer to extract total protein, and protein concentrations were measured using a bicinchoninic acid protein assay (PIERCE, Rockford, IL). Protein lysates (40 μg total) from treated and untreated cells were resolved using 10% sodium dodecyl-sulfate polyacrylamide gel electrophoresis and transferred onto a nitrocellulose membrane. Membranes were incubated with the following primary antibodies (Cell Signaling Technology, Danvers, MA) at 1:1000 dilution in 3% nonfat dry milk: anti-OGDH (#26865) and anti–pyruvate dehydrogenase (#3205). Anti-β-actin (#sc-47778; Santa Cruz Biotechnology, Santa Cruz, CA) was used at a dilution of 1:3000. Incubation with 1:2000 diluted horseradish peroxidase-linked secondary antibodies (#7074/7076; Cell Signaling, Danvers, MA) in 3% nonfat dry milk was subsequently performed at room temperature for 1 hour. The signal was detected using the ECL chemiluminescence detection system (Thermo Fisher Scientific).
Khan H.Y., Kamgar M., Aboukameel A., Bannoura S., Chung B.Y., Li Y., Hallak M.N., Philip P.A., Tsai S., Luther S., Hall W.A, & Azmi A.S. (2022). Targeting Cellular Metabolism With CPI-613 Sensitizes Pancreatic Cancer Cells to Radiation Therapy. Advances in Radiation Oncology, 8(1), 101122.
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2

Immunofluorescent Imaging of Metabolic Enzymes

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Cells were seeded on glass chamber slides coated with fibronectin for neonatal myocytes and hiPSC-CM, or with laminin for adult myocytes, fixed with 3% formaldehyde / 0.3% triton x-100, then incubated with antibodies (1:100) or phalloidin (in Tris-buffered saline with 1% bovine serum albumin), washed and mounted using Prolong Gold anti-fade with DAPI (Molecular Probes). The antibodies included: anti-OGDH (Cell Signaling Technology, same as those listed for the Western blotting, above), as well as, anti-OGDH (Sigma, cat # HPA019514), and anti-ACAA2 (Origene Technologies, cat # TA506126). The slides were imaged using Nikon A1R laser scanning confocal microscope with Plan Apo 60x objective.
Choi S., Pfleger J., Jeon Y.H., Yang Z., He M., Shin H., Sayed D., Astrof S, & Abdellatif M. (2019). Oxoglutarate dehydrogenase and acetyl-CoA acyltransferase 2 selectively associate with H2A.Z-occupied promoters and are required for histone modifications. Biochimica et biophysica acta. Gene regulatory mechanisms, 1862(10), 194436.
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