All the data were analyzed by 1-way analysis of variance using SPSS 22.0 statistical package for Windows (2010, SPSS Inc., Chicago, IL 60606-6307). Differences among treatments were examined using Duncan's multiple range test. Calculated ΔCt (corrected sample) = mean value of target gene-mean value of internal reference gene, ΔΔCt = ΔCt-mean value of control group. Differences with treatment means with a probability of P < 0.05 were accepted as statistically significant.
Spss 22.0 statistical package for windows
Manufactured by IBM
Sourced in United States
SPSS 22.0 is a statistical software package for Windows. It provides a comprehensive set of tools for data analysis, including data manipulation, statistical modeling, and reporting. The software is designed to assist users in managing and analyzing data from various sources.
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5 protocols using spss 22.0 statistical package for windows
1
Gene Expression Analysis by qRT-PCR
2
Abiraterone Acetate Treatment Outcomes
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The primary outcome measure of this investigation was to investigate the frequency of any PSA decline from baseline. Any PSA decline was defined as a PSA decrease from baseline, regardless of degree of decrease during subsequent AA treatment. The secondary outcome measures were PFS and overall survival (OS) with subsequent AA treatment. PFS was defined as the time from the initiation of AA treatment to PSA progression or radiographic progression according to PCWG2 criteria [19 (link)], or clinical progression. OS was defined as the time from initiation of AA to death from any cause or censoring on 30 November 2015. Kaplan–Meier estimates were used for PFS and OS. A correlation analysis between factors were evaluated using Kendall tau or Pearson correlation test, where appropriate. All tests were two-sided and considered significant at p < 0.05. All statistical analyses were performed using SPSS 22.0 statistical package for Windows (SPSS, IBM, Chicago, IL., USA).
3
Statistical Analysis of Experimental Data
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Data are presented as mean ± sd. The significance of differences between groups was determined by Student’s t-test for paired experiments. Two-way ANOVA (with Bonferroni or Tukey’s post hoc test) were used for multiple group comparisons. The Wilcoxon paired samples test was used to assess intergroup differences for cardiac function parameters. Calculations were performed using the SPSS 22.0 statistical package for Windows (SPSS, Inc., Chicago, IL, USA) or Prism 6.0 (GraphPad software, San Diego, CA, USA), and values of p < 0.05 were considered statistically significant.
4
Prognostic Value of BCL6B Methylation in HCC
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We compared baseline clinicopathological characteristics between the BCL6B methylated and unmethylated groups in patients with HCC using the t-test or Chi-square test. Relationships between BCL6B methylation and clinicopathological characteristics were compared using correlation analysis. Univariate and multivariate Cox regression analysis were performed to assess the prognostic value of BCL6B methylation. We calculated odds ratios (ORs) for risk of metastasis and OS in HCC following thermal ablation using unconditional logistic regression, progressively adjusted for age, sex, differential, size, number, hepatitis, virus load, HG, ACL, AST, ALT, WBC, PLA, CHE, ALB, PT, GGT, AFP, and Child-Pugh stratification. The association of BCL6B methylation with metastasis and OS was studied using the Kaplan-Meier method with the log-rank test. All statistical analyses were performed using the SPSS 22.0 statistical package for Windows (SPSS, Chicago, IL, USA) by doctors X. L. C. A. and J. Y. P < 0.05 was considered statistically significant.
5
Exercise Effects on Brain Antioxidants
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Data were analysed using SPSS 22.0 statistical package for Windows (SPSS Inc, USA). The Shapiro-Wilk test was used to check for normality of distribution of data. Parameters which were not normally distributed were transformed for normal distribution prior to statistical analyses. Analysis on the effect of exercise bouts on brain GSH were assessed using one-way repeated measure analyses of variance (ANOVA). Analyses on the effect of two exercise trials (HII and moderate exercise trial) at each of the three time points (pre-, immediately post-and post (~1hour)-exercise) on markers of oxidative stress and BDNF levels in the blood/plasma were performed using a two-way repeated measure ANOVA. Where appropriate, post hoc analyses were conducted to explore the main effects in more detail. The effect of HII exercise on MCAv during the high intensity phase was assessed using one-way repeated measure ANOVA. Statistical significance was accepted at the p<0.05 level and results are presented as means ± standard deviation.
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