Microplate reader at 450 nm

Manufactured by Molecular Devices

Sourced in United States

The Microplate reader at 450 nm is a laboratory instrument used to measure the absorbance of samples in a microplate format. It detects and quantifies the absorption of light at a wavelength of 450 nanometers, which is commonly used for various analytical techniques such as enzyme-linked immunosorbent assays (ELISA).

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dmem medium, by Thermo Fisher Scientific (1 mentions) Cck 8 solution, by Yeasen (1 mentions) Cck 8, by Apexbio (1 mentions) Transam nrf2 assay kit, by Active Motif (1 mentions)

3 protocols using microplate reader at 450 nm

Evaluating MUC-1 Inhibitor in IEC-6 Cells

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Rat intestinal epithelial cell line No. 6 (IEC-6 cells) (iCell-r016, iCellbioscience, Shanghai, China) were cultured in DMEM medium (Gibco), supplied with 10% foetal bovine serum (FBS, Gibco) and 0.1U/mL insulin (MedChemExpress, MCE, Monmouth Junction, NJ, USA) based on ATCC protocol. Cells were seeded in 96-well plates at a density of 1×10⁴/cm². The MUC-1 gene inhibitor GO-203 (MCE) was added to IEC-6 cells with different concentrations (10, 5, and 2.5µM). Moreover, ferrostatin-1 (fer-1, MCE) was added was added at a concentration of 1 µM with 5µM GO-203 to determine whether it could improve the cell viability. Then, 10µL of a CCK-8 solution (YEASEN, Shanghai, China) was added to each well and incubated at 37°C in the dark for 2h. The optical density (OD) levels were measured by microplate reader at 450nm (Molecular Devices).

Wang H., Sun Y., Xiao F.J., Zhao X., Zhang W.Y., Xia Y.J, & Wang L.S. (2023). Mesenchymal Stem Cells Ameliorate DSS-Induced Experimental Colitis by Modulating the Gut Microbiota and MUC-1 Pathway. Journal of Inflammation Research, 16, 2023-2039.

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NK-92 Cell Proliferation Assay

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The cell counting KIT-8 assay (CCK-8, WST-8[2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2 H-tetrazolium, monosodium salt]) (APExBIO, Houston, TX, USA) was used to assess NK-92 cell proliferation under various conditions. Briefly, parental NK-92 cells (1 × 10⁴ cells per well) were either engineered by the Cy3-PDGC21-T-2xC18 conjugates (under identical conditions to produce ApEn-NK cells), treated with equal doses of PDGC21-T aptamer, or not treated (controls). After treatment, cells were seeded in 96-well plates with complete NK culture medium and grown in the cell incubator for 24, 48, or 72 h. At each time point, 10 μL of CCK-8 solution was added to 100 μL cell suspension (1 × 10⁴ cells per well) in each well and the mixture was incubated at 37°C for 2 h. The absorbance in each well was measured by a microplate reader at 450 nm (Molecular Devices, San Jose, CA, USA). Each experiment was performed in triplicate. Background readings from the media were subtracted from the well readings to standardize the results.

Chen Z., Zeng Z., Wan Q., Liu X., Qi J, & Zu Y. (2021). Targeted immunotherapy of triple-negative breast cancer by aptamer-engineered NK cells. Biomaterials, 280, 121259.

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Measuring Nrf2 DNA Binding via TransAM Assay

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The DNA binding of Nrf2 was measured using a specific TransAM^® Nrf2 assay kit (Active Motif), according to the manufacturer’s instructions. Briefly, the nuclear extracts were incubated in the oligonucleotide-coated wells. Subsequently, the wells were washed twice with PBS and incubated with antibody against Nrf2 at 37°C. The addition of secondary antibody conjugated to HRP provided a colorimetric readout. The absorbance of each well was measured using a microplate reader at 450 nm (Molecular Devices).

JEONG S.O., SON Y., LEE J.H., CHEONG Y.K., PARK S.H., CHUNG H.T, & PAE H.O. (2015). Resveratrol analog piceatannol restores the palmitic acid-induced impairment of insulin signaling and production of endothelial nitric oxide via activation of anti-inflammatory and antioxidative heme oxygenase-1 in human endothelial cells. Molecular Medicine Reports, 12(1), 937-944.

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