Chitosan (C) (≥ 75% deacetylated and low molecular weight), melatonin (Mel) and streptozotocin (STZ) were purchased from Sigmaaldrich, UK. Lecithin (L) (lipoid S45) was purchased from Lipoid GmbH, Germany).
Melatonin mel
Manufactured by Merck Group
Sourced in United States, Germany
Melatonin (MEL) is a chemical compound that is naturally produced in the human body. It is a hormone that regulates the sleep-wake cycle and is involved in various physiological processes. MEL is used as a laboratory reagent for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Stf 083010, by Selleck Chemicals (2 mentions)
Thapsigargin tha, by Enzo Life Sciences (2 mentions)
Tunicamycin tun, by Enzo Life Sciences (2 mentions)
Salubrinal sal, by Bio-Techne (2 mentions)
Streptozotocin (stz), by Merck Group (2 mentions)
Fluoxetine flx, by Merck Group (1 mentions)
Imipramine imi, by Merck Group (1 mentions)
Cobalt chloride cocl2, by Merck Group (1 mentions)
Streptozotocin (stz), by Santa Cruz Biotechnology (1 mentions)
Roflumilast rof, by Merck Group (1 mentions)
Nci h292 cell line, by American Type Culture Collection (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Rpmi 1640, by Welgene (1 mentions)
Milli q system, by Merck Group (1 mentions)
Nitroblue tetrazolium, by Merck Group (1 mentions)
Tris hcl, by Merck Group (1 mentions)
N 1 naphthyl ethylenediamine, by Merck Group (1 mentions)
Thiobarbituric acid tba, by Merck Group (1 mentions)
Trichloroacetic acid, by Merck Group (1 mentions)
8 protocols using melatonin mel
1
Chitosan-Melatonin-Streptozotocin Formulation
2
Antidepressant Effects of Melatonin, Fluoxetine, and Imipramine
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All drugs were intraperitoneally (i.p.) administered in a total volume of 10.0 mL/kg (body weight). Doses are expressed as milligrams per kilogram of mouse body weight. Melatonin (MEL) (Sigma-Aldrich Corp., St. Louis, MO, USA) was dissolved in the minimum amount of absolute ethanol and then in isotonic (0.9%) saline solution. Ethanol concentration in that solution was 0.006%, so the vehicle (VEH) tested had that same amount of ethanol. Fluoxetine (FLX) and imipramine (IMI) (Sigma-Aldrich Corp., St. Louis, MO, USA) were dissolved in isotonic saline solution. Experiment series comprised independent groups of 8 animals for each specified drug treatment and behavioral test.
Single administration at ZT11 was as follows: Either MEL or its VEH were administered at ZT11 (1 h before the lights were off), and behavioral tests were assessed 7.5 h later (ZT18.5).
Single administration at ZT18 was as follows: Drugs were administered at ZT18 (the middle of the dark phase), 30 min before the behavioral tests.
Triple scheme administration (for the FST only) was as follows: First administration of the drugs was at 24.5 h before the test (ZT18; immediately after the pre-test session); the second injection was applied one hour before lights were off (ZT11; 7.5 h before the test); finally, the third administration was at ZT18, 30 min before the test [39 (link)].
Single administration at ZT11 was as follows: Either MEL or its VEH were administered at ZT11 (1 h before the lights were off), and behavioral tests were assessed 7.5 h later (ZT18.5).
Single administration at ZT18 was as follows: Drugs were administered at ZT18 (the middle of the dark phase), 30 min before the behavioral tests.
Triple scheme administration (for the FST only) was as follows: First administration of the drugs was at 24.5 h before the test (ZT18; immediately after the pre-test session); the second injection was applied one hour before lights were off (ZT11; 7.5 h before the test); finally, the third administration was at ZT18, 30 min before the test [39 (link)].
3
Induction and Inhibition of ER Stress
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chemical hypoxia was induced with Cobalt Chloride (CoCl2, Merck, Darmstadt, Germany).
ER stress was induced with HA15 (Selleckchem, Zurich, Switzerland), Thapsigargin (THA, Enzo LifeSciences, Lausen, Switzerland), or Tunicamycin (TUN, Enzo LifeSciences, Lausen, Switzerland).
ER stress was inhibited by 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride (AEBSF; Sigma, Darmstadt, Germany), Melatonin (Mel; Sigma, Darmstadt, Germany), STF-083010 (STF; Selleckchem, Zurich, Switzerland), 4μ8C (Selleckchem, Zurich, Switzerland), GSK2656157 (GSK; Selleckchem, Zurich, Switzerland) or Salubrinal (SAL; Tocris Biosciences, Bristol, UK).
ER stress was induced with HA15 (Selleckchem, Zurich, Switzerland), Thapsigargin (THA, Enzo LifeSciences, Lausen, Switzerland), or Tunicamycin (TUN, Enzo LifeSciences, Lausen, Switzerland).
ER stress was inhibited by 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride (AEBSF; Sigma, Darmstadt, Germany), Melatonin (Mel; Sigma, Darmstadt, Germany), STF-083010 (STF; Selleckchem, Zurich, Switzerland), 4μ8C (Selleckchem, Zurich, Switzerland), GSK2656157 (GSK; Selleckchem, Zurich, Switzerland) or Salubrinal (SAL; Tocris Biosciences, Bristol, UK).
4
Streptozotocin and Melatonin in Neuroprotection
Check if the same lab product or an alternative is used in the 5 most similar protocols
The dose and hour of administration of melatonin (dose of 10 mg/kg) were based on previous studies that described its neuroprotective effects (Luengo et al., 2019 ; Rong et al., 2020 ; Omeiza et al., 2021 ). It was dissolved in propylene glycol and diluted in saline, resulting in a final ratio of 5:95 propylene glycol/saline. The final concentration of suspended melatonin was 10 mg/ml, which was infused intraperitoneally (i.p). The control groups received 1 ml/kg of a 5% propylene glycol saline solution.
+ Expand
streptozotocin (STZ; Santa Cruz Biotechnology Inc,. Califórnia, EUA).
Melatonin (MEL; Sigma-Aldrich, St. Louis, MO, USA).
The dose and hour of administration of melatonin (dose of 10 mg/kg) were based on previous studies that described its neuroprotective effects (Luengo et al., 2019 ; Rong et al., 2020 ; Omeiza et al., 2021 ). It was dissolved in propylene glycol and diluted in saline, resulting in a final ratio of 5:95 propylene glycol/saline. The final concentration of suspended melatonin was 10 mg/ml, which was infused intraperitoneally (i.p). The control groups received 1 ml/kg of a 5% propylene glycol saline solution.
5
Evaluating Roflumilast and Melatonin Effects
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The NCI-H292 cell line was obtained from the ATCC (Manassas, VA, USA). Cells were cultured in RPMI 1640 (WELGENE, Gyeongsangbuk-do, Republic of Korea) supplemented with 10% heat-inactivated fetal bovine serum and antibiotics (WELGENE). The viability assay was performed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich, Saint Louis, MO, USA). Cells were maintained in 96-well plates at a density of 3 × 104 cells/well. Roflumilast (ROF, Sigma-Aldrich) and melatonin (MEL, Sigma-Aldrich) were added to each well as follows: ROF: 2.5, 5, 10, and 20 μM; MEL: 100, 200, 400, and 800 μM. This was followed by incubation for 24 h. MTT solution (10 μL) was added to each well, and the cells were incubated for 4 h at 37 °C; then, 100 μL of dimethyl sulfoxide (DMSO, Sigma-Aldrich) was added to each well to solubilize the formazan produced. The optical density of the culture was measured at 570 nm.
6
Melatonin Purification and Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All chemicals were of the “high purity” category from the Alamed company, Moscow, Russia and from the Sigma-Aldrich company, St. Louis, MO, USA. All solutions were prepared in deionized water obtained from the Milli-Q system (Millipore, Bedford, MA, USA). Melatonin (MEL) was obtained from Sigma-Aldrich, St. Louis, MO, USA.
7
Melatonin Alleviates Aluminum Chloride-Induced Oxidative Stress
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Anhydrous aluminium chloride (AlCl 3 ; CAS Number 7446-70-0), melatonin (Mel; CAS Number 73-31-4), nitro blue tetrazolium, N-(1-naphthyl) ethylenediamine, Thiobarbituric acid (TBA) and Tris-HCl were purchased from Sigma (St. Louis, MO, USA). Thiobarbituric acid and trichloroacetic acid were purchased from Merck (Darmstadt, Germany). All other chemicals and reagents used in this study were of analytical grade. Doubledistilled water was used as the solvent.
Adult male Wistar albino rats weighing 150-180 g were obtained from the Holding Company for Biological Products and Vaccines (VACSERA, Cairo, Egypt). After an acclimatization period of 1 week, the animals were divided into four groups (seven rats per group) and housed in wire-bottomed cages in a room under standard condition of illumination with a 12-h light/dark cycle at 25 ± 2°C. The rats were provided water and a balanced diet ad libitum. We followed the European Community Directive (86/609/EEC) and national rules on animal care that was carried out in accordance with the NIH Guidelines for the Care and Use of Laboratory Animals 8th edition.
Adult male Wistar albino rats weighing 150-180 g were obtained from the Holding Company for Biological Products and Vaccines (VACSERA, Cairo, Egypt). After an acclimatization period of 1 week, the animals were divided into four groups (seven rats per group) and housed in wire-bottomed cages in a room under standard condition of illumination with a 12-h light/dark cycle at 25 ± 2°C. The rats were provided water and a balanced diet ad libitum. We followed the European Community Directive (86/609/EEC) and national rules on animal care that was carried out in accordance with the NIH Guidelines for the Care and Use of Laboratory Animals 8th edition.
8
Modulation of ER Stress Responses
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chemical hypoxia was induced with Cobalt Chloride (CoCl 2 , Merck, Darmstadt, Germany).
ER stress was induced with HA15 (Selleckchem, Zurich, Switzerland), Thapsigargin (THA, Enzo LifeSciences, Lausen, Switzerland), or Tunicamycin (TUN, Enzo LifeSciences, Lausen, Switzerland). ER stress was inhibited by 4-(2-aminoethyl) benzenesulfonyl uoride hydrochloride (AEBSF; Sigma, Darmstadt, Germany), Melatonin (Mel; Sigma, Darmstadt, Germany), STF-083010 (STF; Selleckchem, Zurich, Switzerland), 4µ8C (Selleckchem, Zurich, Switzerland), GSK2656157 (GSK; Selleckchem, Zurich, Switzerland) or Salubrinal (SAL; Tocris Biosciences, Bristol, UK).
ER stress was induced with HA15 (Selleckchem, Zurich, Switzerland), Thapsigargin (THA, Enzo LifeSciences, Lausen, Switzerland), or Tunicamycin (TUN, Enzo LifeSciences, Lausen, Switzerland). ER stress was inhibited by 4-(2-aminoethyl) benzenesulfonyl uoride hydrochloride (AEBSF; Sigma, Darmstadt, Germany), Melatonin (Mel; Sigma, Darmstadt, Germany), STF-083010 (STF; Selleckchem, Zurich, Switzerland), 4µ8C (Selleckchem, Zurich, Switzerland), GSK2656157 (GSK; Selleckchem, Zurich, Switzerland) or Salubrinal (SAL; Tocris Biosciences, Bristol, UK).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!