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Lysozymec

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Lysozyme C is a laboratory enzyme used for the lysis of bacterial cell walls. It functions by hydrolyzing the glycosidic bonds in the peptidoglycan layer of bacterial cell walls, leading to cell disruption and death. Lysozyme C is commonly used in various microbiological and biochemical applications.

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Lab products found in correlation

Anti flag, by Merck Group (1 mentions) F3165, by Merck Group (1 mentions) Anti tgn46, by Bio-Rad (1 mentions) Anti gm130, by BD (1 mentions) Ahp500g, by Bio-Rad (1 mentions) Anti ha, by Roche (1 mentions) Ab74524, by Abcam (1 mentions) Anti comp, by Abcam (1 mentions) Microamp fast optical 96 well reaction plate, by Thermo Fisher Scientific (1 mentions) Human serum albumin (hsa), by Merck Group (1 mentions) Catalase, by Merck Group (1 mentions) Cytochrome c, by Merck Group (1 mentions) Conalbumin, by Merck Group (1 mentions) Ovotransferrin, by Merck Group (1 mentions) Myoglobin, by Merck Group (1 mentions)

3 protocols using lysozymec

1

COMP Protein Immunofluorescence and STORM Analysis

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The commercial antibodies used in this study, and their sources, are as follows: Anti-COMP (rabbit polyclonal, ab74524; Abcam), anti-FLAG (mouse monoclonal, F3165; Sigma-Aldrich), and anti-TGN46 (sheep polyclonal, AHP500G; AbD Serotec) were used for WB, together with the secondary antibodies sheep (sc-2770, sc-2303, rabbit sc-2301 and mouse sc-2314) obtained from Santa Cruz Biotechnology, Inc. Anti-HA (rat monoclonal, 11867423001; Roche), anti-FLAG (mouse monoclonal; F3165; Sigma-Aldrich), anti-GM130 (mouse monoclonal, 610822; BD Biosciences), and anti-ATP2C1 (mouse monoclonal, HPA035116; Sigma-Aldrich) were used for immunofluorescence microscopy. The secondary antibodies Alexa Fluor 488 rabbit (A21206), Alexa Fluor 594 mouse (A21203), and Alexa Fluor 488 rat (A21208) were purchased from Thermo Fisher Scientific. For STORM measurements Cy5-conjugated AffiniPure (rabbit, 711–175-152; Jackson ImmunoResearch Laboratories, Inc.) was used for Cab45 as a secondary antibody and Alexa Fluor 488 sheep (A11015) for TGN46 (Thermo Fisher Scientific). The recombinant proteins COMP-FLAG (COMP-3659H; Creative BioMart), COMP (ab104358; Abcam), LysozymeC (Sigma-Aldrich), and LysozymeC-Cy5 (LS1-S5-1; Nanocs) were used for in vitro analysis.
Crevenna A.H., Blank B., Maiser A., Emin D., Prescher J., Beck G., Kienzle C., Bartnik K., Habermann B., Pakdel M., Leonhardt H., Lamb D.C, & von Blume J. (2016). Secretory cargo sorting by Ca2+-dependent Cab45 oligomerization at the trans-Golgi network. The Journal of Cell Biology, 213(3), 305-314.
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2

Lysozyme C Solubilization and Dilution

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Lysozyme C (UNIPROT ID: P00698) was purchased from Sigma Aldrich (L6876). Lyophilized lysozyme powder was solubilized in PBS buffer (NaCl, 137 mM; KCl 2.7 mM; Na2HPO4, 10 mM; and KH2PO4, 1.8 mM) pH 7.4 to a final concentration of 10 mg/ml. A series of two-fold dilutions in PBS was prepared in a MicroAmp Fast Optical 96-Well Reaction Plate (4346906) from Applied Biosystems.
Hofmann L., Gulati S., Sears A., Stewart P.L, & Palczewski K. (2016). An effective thiol-reactive probe for differential scanning fluorimetry with a standard RT-PCR device. Analytical biochemistry, 499, 63-65.
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3

Protein Mix Characterization Protocol

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The seven-protein mix comprised human serum albumin (HSA), cytochrome C (bovine heart), ovotransferrin (Conalbumin, chicken egg white), myoglobin (equine heart), lysozyme C (chicken egg white), and catalase (bovine liver), all purchased individually from Sigma Aldrich (St. Louis, MO). Creatine kinase Type M (rabbit muscle) was purchased from Roche (Basel, Switzerland). The cross-linker BS3 was purchased from Thermo Scientific Pierce (Rockford, IL).
Müller F., Kolbowski L., Bernhardt O.M., Reiter L, & Rappsilber J. (2019). Data-independent Acquisition Improves Quantitative Cross-linking Mass Spectrometry. Molecular & Cellular Proteomics : MCP, 18(4), 786-795.
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