The butanol fraction was subjected to isolation using preparative thin layer chromatography (PTLC). The PTLC plates were prepared (coated) using silica gel GF 254 (Loba Chemie, India), automatic TLC plate coater (Camag, Switzerland), distilled water, and 20 × 20 cm TLC glass plates (Camag, Switzerland). Chloroform: n-hexane: methanol (4 : 1 : 0.25) was used as mobile phase. The developed chromatograph was viewed under UV lamp (Uvitec, UK) at wavelengths of 254 and 365 nm [24 ]. The chromatographic zone was coded as A, B, C, and D based on the ascending order of retention factor (RF) values of bands. Each band was carefully marked with pencil, scrapped, collected, and dissolved in chloroform and methanol (1 : 1). The resulting mixture was filtered and the solvent was evaporated using a drying oven [24 , 25 ]. Finally, purity of the isolated compounds was monitored using analytical TLC plate (Merck, Germany) by viewing under UV lamp at the specified wavelengths. Mixed or contaminated compounds were repurified.
Tlc glass plates
Manufactured by CAMAG
Sourced in Switzerland, Germany
TLC glass plates are a type of laboratory equipment used for thin-layer chromatography (TLC) analysis. They provide a solid support for the stationary phase, which is typically a thin layer of adsorbent material coated onto the surface of the glass plate. The TLC glass plates serve as the platform for the separation and analysis of complex mixtures of compounds.
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2 protocols using tlc glass plates
1
Isolation and Purification of Bioactive Compounds via PTLC
2
Analytical Thin Layer Chromatography
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Analytical TLC glass plates, TLC jar, measuring cylinder, volumetric flask, UV cabinet, automatic TLC coater were obtained from CAMAG (Berlin, Germany); electronic balance and vacuum oven were obtained from Daihan (Seoul, South Korea); glucometer and test strips were purchased from Care sense N (Seoul, South Korea).
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