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Cd64 pecy7

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The CD64-PeCy7 is a flow cytometry reagent that targets the CD64 antigen, also known as the Fc gamma RI receptor. It is conjugated with the PeCy7 fluorochrome, which allows for the detection and analysis of CD64-expressing cells. The CD64 antigen is expressed on the surface of various immune cells, including monocytes, macrophages, and neutrophils, and is involved in the binding and phagocytosis of antibody-coated targets.

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Lab products found in correlation

Ly6c percp cy5, by Thermo Fisher Scientific (1 mentions) Cd103 pe, by Thermo Fisher Scientific (1 mentions) Pd 1 fitc, by Thermo Fisher Scientific (1 mentions) Cd4 bv510, by Thermo Fisher Scientific (1 mentions) Nk1.1 apc cy7, by Thermo Fisher Scientific (1 mentions) Cd3 a700, by Thermo Fisher Scientific (1 mentions) Collagenase type 1, by Merck Group (1 mentions) Cd8 apc, by Thermo Fisher Scientific (1 mentions) Ly6g fitc, by Thermo Fisher Scientific (1 mentions) Cd19 percp cy5, by Thermo Fisher Scientific (1 mentions) Cd44 pe, by Thermo Fisher Scientific (1 mentions) Mhcii apc, by Thermo Fisher Scientific (1 mentions) Dnase 1, by Merck Group (1 mentions) Cd11b percp cy5, by Thermo Fisher Scientific (1 mentions) Cd45.2 pe, by Thermo Fisher Scientific (1 mentions) F4 80 apc, by Thermo Fisher Scientific (1 mentions) Cd11c alexa fluor 647, by Thermo Fisher Scientific (1 mentions) Annexin v 7 aad, by Thermo Fisher Scientific (1 mentions) Gr 1 pe, by Thermo Fisher Scientific (1 mentions) Dnase 1, by Roche (1 mentions)

2 protocols using cd64 pecy7

1

Lung Cell Isolation and Immunophenotyping

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Single-cell suspensions of the lung tissues were prepared by cutting them into small pieces followed by incubation in Dulbecco’s Modified Eagle Media (DMEM) containing 0.18 mg/mL Collagenase Type I (Sigma, St. Louis, MO, USA), 0.02 mg/mL DNase I (Sigma, St. Louis, MO, USA) for 1 h at 37 °C under constant rotation, followed by being mechanically passed through a 100 μm and 70 μm cell strainer sequentially. Erythrocytes were lysed using RBC lysis buffer (155 mM NH4Cl, 12 mM NaHCO3, 0.1 mM EDTA). Cells were then counted and subjected to flow cytometry. Lymphoid and myeloid compartments were investigated in the lung samples of mice on various intervention diets. Antibodies used for flow cytometry analysis were as follows: CD64-PeCy7 (Clone X54-5/7.1), Ly6C-PerCPCy5.5 (Clone AL-21), CD11b-V450 (Clone M1/70), MHCII-APC (Clone M5/114.15.2), CD103-PE (Clone M290), CD11c-A700 (Clone HL3), SiglecF-APCCy7 (Clone E5-2440), Ly6G-FITC (Clone 1A8), PD-1-FITC (Clone 29F.1A12), CD4-BV510 (Clone RM4-5), CD44-PE (Clone IM7), NK1.1-APCCy7 (Clone PK136), CD3-A700 (Clone 500A2), CD62L-V450 (Clone MEL-14), CD19-PerCPCy5.5 (Clone 1D3), CD8-APC (Clone 53-6.7), and KLRG1-BV786 (Clone 2F1) purchased from BD (Biosciences, Johannesburg, SA) and eBioscience (ThermoFisher, Johannesburg, SA) [29 (link),30 (link)].
Nienaber A., Baumgartner J., Dolman R.C., Ozturk M., Zandberg L., Hayford F.E., Brombacher F., Blaauw R., Parihar S.P., Smuts C.M, & Malan L. (2020). Omega-3 Fatty Acid and Iron Supplementation Alone, but Not in Combination, Lower Inflammation and Anemia of Infection in Mycobacterium tuberculosis-Infected Mice. Nutrients, 12(9), 2897.
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2

Isolation and Analysis of Alveolar Macrophages

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Cells were collected from digested lung samples [collagenase D (1 mg/ml) and DNase I (0.1 mg/ml) (Roche, Indianapolis, IN)] prior to staining with CD45.2-PE, CD11b-PERCP Cy5.5, CD11c-Alexa Fluor 647, CD64-PE-Cy7, F4/80-APC, and/or GR1-PE (eBioscience, San Diego, CA) in staining solution (1% FBS supplemented 1× PBS). Alveolar macrophage populations were analyzed using previously published methods (23 (link)). Briefly, CD45+ cell populations were initially selected and CD11c+CD11b-CD64+ cells were quantified as alveolar macrophages. Non-determined macrophage populations were identified as F4/80+CD11b+GR1- cells. Examination of apoptosis was performed using Annexin V/7-AAD staining per manufacturer's instructions (eBioscience, San Diego, CA). All flow cytometry was analyzed using FlowJo software (Tree Star, Inc., Ashland, OR).
Mitzel D.N., Lowry V., Shirali A.C., Liu Y, & Stout-Delgado H.W. (2014). Age Enhanced ER Stress Contributes to Increased Atg9A Inhibition of STING Mediated IFNβ Production during Streptococcus Pneumoniae Infection. Journal of immunology (Baltimore, Md. : 1950), 192(9), 4273-4283.
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