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H9 hesc wa09 line

Manufactured by WiCell
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The H9 hESC (WA09) line is a human embryonic stem cell line derived from a blastocyst-stage embryo. It is maintained and distributed by WiCell as a research tool for the scientific community.

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Lab products found in correlation

Interleukin 6 (il 6), by Thermo Fisher Scientific (2 mentions) Vascular endothelial growth factor (vegf), by Thermo Fisher Scientific (2 mentions) Stem cell factor (scf), by Thermo Fisher Scientific (2 mentions) Fibroblast growth factor 2 (fgf2), by Thermo Fisher Scientific (2 mentions) Lithium chloride (licl), by Merck Group (2 mentions) Bone morphogenetic protein 4 (bmp4), by Thermo Fisher Scientific (2 mentions) Interleukin 3 (il 3), by Thermo Fisher Scientific (2 mentions) Activin a, by Thermo Fisher Scientific (2 mentions) Mtesr1 media, by WiCell (2 mentions) Tgf β inhibitor, by Cayman Chemical (2 mentions) Rock inhibitor, by Cayman Chemical (2 mentions)

Close spelling variants of this product

H9 hESCs (65 citations) H9/WA09 (33 citations) H9 hESC line (25 citations) H9 line (8 citations) WA09 hESCs (6 citations) HESCs (H9) (5 citations) WA09 hESC line (2 citations) WA09 line (2 citations)

2 protocols using h9 hesc wa09 line

1

Directed Differentiation of hPSCs to Hematopoietic Lineages

Cited 1 time
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hPSCs (H9 hESC (WA09) line from WiCell), iSOX17 H9 line and knockout SOX17 H9 line were maintained and passaged on Matrigel in mTeSR1 media (WiCell). The cell lines were differentiated on collagen IV (ColIV)-coated plate (Uenishi et al., 2014 (link)). Cell lines were plated at a density of 5,000 cells/cm2 onto 6 well plates with E8 media containing 10 μM Rock inhibitor (Y-27632, Cayman Chemicals). The following day, the media was changed to IF9S media with 50 ng/ml FGF2 (PeproTech), 50 ng/ml BMP4 (PeproTech), 15 ng/ml Activin A (PeproTech), and 2 mM LiCl (Sigma), and cultured in hypoxia (5% CO2, 5% O2). On day 2, the media was changed to IF9S media with 50 ng/ml FGF2, 50 ng/ml VEGF (PeproTech), and 2.5 μM TGF-β inhibitor (SB-431542, Cayman), and cultured in hypoxia (5% CO2, 5% O2). On days 4 and 6, the media was changed to IF9S media with 50 ng/ml FGF2, 50 ng/ml VEGF, 50 ng/ml TPO (PeproTech), 50 ng/ml IL-6 (PeproTech), 20 ng/ml SCF (PeproTech), and 10 ng/ml IL-3 (PeproTech), and cultured in normoxia (5% CO2, 20% O2). DOX (Sigma) was added to cultures on day 2 of differentiation at concentration of 2 μg/ml.
Jung H.S., Uenishi G., Park M.A., Liu P., Suknuntha K., Raymond M., Choi Y.J., Thomson J.A., Ong I.M, & Slukvin I.I. (2021). SOX17 integrates HOXA and arterial programs in hemogenic endothelium to drive definitive lympho-myeloid hematopoiesis. Cell reports, 34(7), 108758.
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2

Directed Differentiation of hPSCs to Hematopoietic Lineages

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
hPSCs (H9 hESC (WA09) line from WiCell), iSOX17 H9 line and knockout SOX17 H9 line were maintained and passaged on Matrigel in mTeSR1 media (WiCell). The cell lines were differentiated on collagen IV (ColIV)-coated plate (Uenishi et al., 2014 (link)). Cell lines were plated at a density of 5,000 cells/cm2 onto 6 well plates with E8 media containing 10 μM Rock inhibitor (Y-27632, Cayman Chemicals). The following day, the media was changed to IF9S media with 50 ng/ml FGF2 (PeproTech), 50 ng/ml BMP4 (PeproTech), 15 ng/ml Activin A (PeproTech), and 2 mM LiCl (Sigma), and cultured in hypoxia (5% CO2, 5% O2). On day 2, the media was changed to IF9S media with 50 ng/ml FGF2, 50 ng/ml VEGF (PeproTech), and 2.5 μM TGF-β inhibitor (SB-431542, Cayman), and cultured in hypoxia (5% CO2, 5% O2). On days 4 and 6, the media was changed to IF9S media with 50 ng/ml FGF2, 50 ng/ml VEGF, 50 ng/ml TPO (PeproTech), 50 ng/ml IL-6 (PeproTech), 20 ng/ml SCF (PeproTech), and 10 ng/ml IL-3 (PeproTech), and cultured in normoxia (5% CO2, 20% O2). DOX (Sigma) was added to cultures on day 2 of differentiation at concentration of 2 μg/ml.
Jung H.S., Uenishi G., Park M.A., Liu P., Suknuntha K., Raymond M., Choi Y.J., Thomson J.A., Ong I.M, & Slukvin I.I. (2021). SOX17 integrates HOXA and arterial programs in hemogenic endothelium to drive definitive lympho-myeloid hematopoiesis. Cell reports, 34(7), 108758.
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