Human elisa kits for ifn γ

Oral squamous carcinoma stem cells (OSCSCs) were isolated from patients with tongue tumors at the University of California, Los Angeles (UCLA) [11 (link)] and were cultured in RPMI 1640 (Life Technologies, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS) (Gemini Bio-Product, CA, USA). RPMI 1640 supplemented with 10% FBS was used to culture human NK cells and monocytes. Anti-CD16 mAbs, and flow cytometric antibodies were purchased from Biolegend (San Diego, CA, USA). Recombinant human IL-2 was obtained from Hoffman (La Roche, NJ, USA). AJ2 is a combination of seven different strains of gram-positive probiotic bacteria: Streptococcus thermophiles, Bifidobacterium longum, Bifidobacterium breve, Bifidobacterium infantis, Lactobacillus acidophilus, Lactobacillus plantarum, and Lactobacillus casei. AJ2 were sonicated as described previously [5 (link)]. RPMI 1640 supplemented with 10% FBS was used to re-suspend sonicated AJ2 (sAJ2). Human ELISA kits for IFN-γ were purchased from Biolegend (San Diego, CA, USA). Phosphate buffered saline (PBS) and bovine serum albumin (BSA) were purchased from Life Technologies (Carlsbad, CA, USA).

RPMI 1640 (Life Technologies, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS) (Gemini Bio-Products, West Sacramento, CA, USA) was used for peripheral blood mononuclear cells (PBMCs), NK cells, T cells, monocytes, and oral squamous carcinoma stem cells (OSCSCs) cultures. Recombinant IL-2 was obtained from NIH- BRB. Antibodies to CD16 (clone 3G8) were purchased from Biolegend, San Diego, CA, USA. ImmunoCult-XF T cell expansion medium and Immunocult human CD3/CD28 T cell activator was purchased from Stem Cell Technologies (Vancouver, BC, Canada). OSCSCs were isolated from patients with tongue tumors at UCLA [22 (link),23 (link),24 (link),25 (link)]. Probiotic bacteria, sAJ2 is a combination of 8 different strains and is prepared as described previously [25 (link)], and RPMI 1640 supplemented with 10% FBS was used to re-suspend AJ2. Human ELISA kits for IFN-γ were purchased from Biolegend, San Diego, CA, USA. Chromium-51 radionucleotide was purchased from PerkinElmer, Richmond, CA, USA. The following antibodies with their respective clones were purchased from Biolegend, San Diego, CA, USA and used as suggested by the manufacturer for flow cytometric analysis; CD45 (H130) CD3/16/56: (UCHT1/3G8/MEM-188), CD3 (UCHT1), CD8 (HIT8a), CD14 (63D3) CD19 (HIB19).

RPMI 1640 (Life Technologies, Carlsbad, CA, USA) medium supplemented with 10% fetal bovine serum (FBS (Life Technologies, Carlsbad, CA, USA)) was used to culture the immune cells derived from human and humanized-BLT (hu-BLT) mice. Alpha-MEM (Life Technologies, Carlsbad, CA, USA) medium supplemented with 10% FBS was used to culture OCs. Human M-CSF, monoclonal antibodies for NK cells (anti-CD16 mAbs) and human ELISA kits for IFN-γ were obtained from Biolegend, San Diego, CA, USA. RANKL was obtained from PeproTech, Cranbury, NJ, USA, and recombinant human IL-2 (rh-IL2) was obtained from Hoffman La Roche, Little Falls, NJ, USA. Monoclonal antibodies for T cell human anti-CD3/CD28 mAbs were purchased from Stem Cell Technologies, Kingsway, Vancouver, BC, Canada.