Mouse anti human cd14

Manufactured by BD

Sourced in United States

Mouse anti-human CD14 is a laboratory reagent that can be used to detect the presence of CD14 on the surface of human cells. CD14 is a glycoprotein that acts as a co-receptor for the detection of bacterial lipopolysaccharide. This reagent can be used in various immunological techniques, such as flow cytometry, to study the expression of CD14 on different cell types.

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Lab products found in correlation

Donkey serum, by Merck Group (1 mentions) To pro 3, by Thermo Fisher Scientific (1 mentions) Mowiol, by Merck Group (1 mentions) Donkey anti mouse alexa fluor 488, by Jackson ImmunoResearch (1 mentions) Rhodamine red x donkey anti rabbit, by Jackson ImmunoResearch (1 mentions) Donkey anti mouse cy3, by Jackson ImmunoResearch (1 mentions) Live dead fixable aqua dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Mouse anti human cd3, by BD (1 mentions) Mouse anti human cd16, by BioLegend (1 mentions) Mouse anti human cd45, by BioLegend (1 mentions) Facs lysing solution, by BD (1 mentions) Lsrfortessa, by BD (1 mentions)

2 protocols using mouse anti human cd14

Immunohistochemistry of P-IRE1α in Multiple Sclerosis

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Frozen brain tissue from 4 MS patients and 4 healthy control individuals was cut into 7 μm thick sections, air dried, and fixed in ice-cold acetone for 10 minutes. Sections were delipidised in 70% ethanol for 5 minutes, followed by blocking of non-specific binding with 10% donkey serum (#D9663, Sigma-Aldrich). Rabbit anti-human P-IRE1α (#NB100–2323, Novus Biologicals, 1:50) was incubated with mouse anti-human GFAP-Cy3 (#C9205, Sigma-Aldrich, 1:50), mouse anti-human NeuN (#MAB377, Millipore, 1:50), mouse anti-human CNPase (#MAB326, Millipore, 1:100) or mouse anti-human CD14 (#555397, BD Biosciences, 1:10) in blocking buffer overnight at 4C. The next day slides were washed with 0.05% PBS-Tween and incubated with a mixture of donkey anti-rabbit Alexa Fluor 488 (#R37118, Life Technologies, 1:400) and donkey anti-mouse-Cy3 (#715–165-151, Jackson ImmunoResearch, 1:200) or donkey anti-rabbit-Rhodamine Red X (#711–295-152, Jackson ImmunoResearch, 1:400) and donkey anti-mouse-Alexa Fluor 488 (Jackson ImmunoResearch, 1:400) for 40 minutes at room temperature. Sections were mounted in Mowiol (#81381, Sigma-Aldrich) containing TOPRO-3 (#T3605, Invitrogen). In secondary only controls, primary antibodies were omitted to control for non-specific binding.

Wheeler M.A., Jaronen M., Covacu R., Zandee S.E., Scalisi G., Rothhammer V., Tjon E.C., Chao C.C., Kenison J.E., Blain M., Rao V.T., Hewson P., Barroso A., Gutiérrez-Vázquez C., Prat A., Antel J.P., Hauser R, & Quintana F.J. (2019). Environmental control of astrocyte pathogenic activities in CNS inflammation. Cell, 176(3), 581-596.e18.

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Quantification of Immune Subsets in Zika Virus Infection

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Whole blood (100 μL) was labelled as previously described.13 Antibodies were used to identify CD45⁺ leucocytes (mouse anti‐human CD45; Biolegend, California, CA, USA), high SSC‐A CD16⁺ neutrophils (mouse anti‐human CD16; Biolegend), CD14⁺ monocytes (mouse anti‐human CD14; BD Biosciences, New Jersey, NJ, USA), CD3⁺ T cells (mouse anti‐human CD3; BD Biosciences), CD4⁺ T helper cells (mouse anti‐human CD4; Thermo Fisher Scientific), CD56⁺ NK cells (mouse anti‐human CD56; Miltenyi Biotec, Bergisch Gladbach, Germany) and CD19⁺ B cells (mouse anti‐human CD19; Thermo Fisher Scientific). Cell fixation and RBC lysis was performed using 1× FACS lysing solution (BD Biosciences), and permeabilisation using 1× FACS permeabilisation solution 2 (BD Biosciences) before staining with a ZIKV NS3 protein‐specific rabbit polyclonal antibody.13 The labelled cells were counter‐stained with a fluorophore‐tagged secondary goat anti‐rabbit IgG (H + L) antibody (Thermo Fisher Scientific), before acquisition on a LSR Fortessa (BD Biosciences). Dead cells were excluded using a Live/Dead™ Fixable Aqua Dead Cell Stain Kit (Thermo Fisher Scientific). The frequencies of peripheral blood immune subsets were obtained with the following formula: Percentage of specific immune subset (obtained from immune‐phenotyping) × total leucocyte number (obtained from CBC) = cellular number of specific immune subset.

Lum F., Narang V., Hue S., Chen J., McGovern N., Rajarethinam R., Tan J.J., Amrun S.N., Chan Y., Lee C.Y., Chua T., Yee W., Yeo N.K., Tan T., Liu X., Haldenby S., Leo Y., Ginhoux F., Chan J.K., Hiscox J., Chong C, & Ng L.F. (2019). Immunological observations and transcriptomic analysis of trimester‐specific full‐term placentas from three Zika virus‐infected women. Clinical & Translational Immunology, 8(11), e01082.

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