Visking

Manufactured by Serva Electrophoresis

Sourced in Germany

VISKING is a dialysis tubing product used for separating molecules based on their size and molecular weight. It is made from regenerated cellulose and is designed for laboratory applications.

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Lab products found in correlation

Slide a lyzer mini dialysis unit, by Thermo Fisher Scientific (1 mentions) Biophotometer, by Eppendorf (1 mentions) Alpha 1 4 ldplus laboratory freeze dryer, by Martin Christ (1 mentions)

Close spelling variants of this product

Visking® dialysis tubing (9 citations) Visking®, MWCO 12,000–14,000; (5 citations)

5 protocols using visking

Protein Dialysis and Turbidity Measurement

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For ThT‐binding and turbidity measurement, specimens were dialyzed in 200 mmol/L acetate buffer containing 100 mmol/L NaCl and 1 mmol/L EDTA at pH 5.0 with Slide‐A‐Lyzer MINI Dialysis Units (Thermofisher Scientific). Afterwards, specimens from multiple columns were pooled and concentration was measured with a BioPhotometer (Eppendorf, Hamburg, Germany). Proteins were diluted with respective buffer to a final concentration of 0.244 mg/mL.
For time course turbidity assay, proteins were dialyzed against phosphate buffer solution (20 mmol/L sodium phosphate, 100 mmol/L NaCl, 1 mmol/L EDTA, pH 7.6) using a dialysis tube (VISKING, SERVA, Heidelberg, Germany) with a volume per length of 2 mL/cm and a molecular weight cut‐off of 12–14 kDa. After dialysis, the OD280 of each TTR‐variant was measured with a BioPhotometer (Eppendorf, Hamburg, Germany) and the gained protein concentrations were equaled by adding buffer (20 mmol/L sodium phosphate, 100 mmol/L NaCl, 1 mmol/L EDTA, pH 7.6), down to a protein concentration of 0.6 mg/mL.

Grether N.B., Napravnik F., Imhof T., Linke R.P., Bräsen J.H., Schmitz J., Dohrn M., Schneider C., Svačina M.K., Stetefeld J., Koch M, & Lehmann H.C. (2022). Amyloidogenicity assessment of transthyretin gene variants. Annals of Clinical and Translational Neurology, 9(8), 1252-1263.

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In Vitro Release Kinetics of Quercetin from SLNs

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Appropriate volumes of QCT-loaded SLNs (equivalent to 1 mg QCT) were placed in dialysis bags (Visking^®, MWCO 12,000–14,000; SERVA, Heidelberg, Germany). The bags were placed in flasks containing 20 mL of release media (PBS with 0.7% Tween 80 to ensure sink conditions, pH 6.2 to mimic that of urine) in a shaking water bath (Wisebath^®, London, UK) at 37 °C, 100 rpm. Samples of the release media were withdrawn at predetermined time intervals and the amount of QCT was determined spectrophotometrically at 375 nm.
After sample collection, the withdrawn medium was replaced with fresh buffer throughout the first 22 h of sampling. Afterwards, the release medium was entirely replaced each time with a new buffer, as previously reported by Patra et al. [39 (link)]. Dialyzed QCT from ethanol solutions was also quantified under the same conditions. To identify the release mechanism, data from release experiments were fitted to different release kinetic models (zero-order, first-order, Hixon Crowel, Higuchi, and Korsmeyer-Peppas). Experiments were done in triplicates.

Shawky S., Makled S., Awaad A, & Boraie N. (2022). Quercetin Loaded Cationic Solid Lipid Nanoparticles in a Mucoadhesive In Situ Gel—A Novel Intravesical Therapy Tackling Bladder Cancer. Pharmaceutics, 14(11), 2527.

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Isolation and Purification of Polysaccharides

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A volume of 500 mL of the obtained extracts (M1-4) was used for the isolation of PS fractions. The extracts were initially centrifuged (20 min, 5000× g). The volume of the supernatant was reduced in half. Then, the supernatant and 96% (v/v) cold ethanol were combined in a ratio of 1:2 to precipitate PSs. The mixture was centrifuged (20 min, 4 °C, 3490× g) to recover the precipitate. It was then re-dissolved in distilled water and extensively dialyzed (mwco 3.5 kDa, Visking^®, SERVA Electrophoresis, Heidelberg, Germany) for 72 h against distilled water (4 °C). Finally, the dialysate was frozen in plastic containers (100 mL), which were freeze-dried in an Alpha 1–4 LDplus laboratory freeze dryer (Martin Christ Gefriertrocknungsanlagen GmbH, Osterode am Harz, Germany).

Atanasova A., Petrova A., Teneva D., Ognyanov M., Georgiev Y., Nenov N, & Denev P. (2023). Subcritical Water Extraction of Rosmarinic Acid from Lemon Balm (Melissa officinalis L.) and Its Effect on Plant Cell Wall Constituents. Antioxidants, 12(4), 888.

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Extraction and Purification of Polysaccharides from Agaricus bisporus

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Fifty grams of AIS from MP fruiting bodies was subjected to a double extraction with boiling ultrapure water (0.055 µS/cm, Adrona Crystal B6.1, Riga, Latvia) for 1 h, employing a ratio of AIS to water of 1:28. The extract was filtered through a nylon cloth and the residue was washed with ultrapure water (150 mL). Then, the extract with the washing water was centrifuged at 4000× g for 25 min at RT. The supernatant was filtered with a Büchner funnel (KA 4 paper filters, Papírna Perštejn s.r.o., Perštejn, Czech Republic) and concentrated 4 times in a rotary vacuum evaporator at 40 °C. The concentrated extract was centrifuged, filtered again and the PSs were coagulated with 95% (v/v) cold ethanol (1:4) at 4 °C overnight. The PSs were recovered by centrifugation (4000× g for 30 min at 4 °C), dissolved in ultrapure water and dialyzed (MWCO 12–14 kDa, VISKING^®, SERVA Electrophoresis GmbH, Heidelberg, Germany) against deionized water for 72 h at 4 °C. Finally, PSs were centrifuged at RT to remove any formed insoluble solids, filtered and freeze-dried. The obtained PSC from MP was named MP-PSC.

Georgiev Y.N., Vasicek O., Dzhambazov B., Batsalova T.G., Denev P.N., Dobreva L.I., Danova S.T., Simova S.D., Wold C.W., Ognyanov M.H., Paulsen B.S, & Krastanov A.I. (2022). Structural Features and Immunomodulatory Effects of Water-Extractable Polysaccharides from Macrolepiota procera (Scop.) Singer. Journal of Fungi, 8(8), 848.

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Extraction and Purification of Microbial EPSs

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Initially, the biomass was separated from the culture suspension by centrifugation at 5000×g for 25 min at 20°C. Afterwards, the volume of supernatant was reduced threefold through a vacuum concentration at 50°C (-0.1MPa). In order to precipitate EPS, 96% (v/v) cold ethanol was added to the concentrated supernatant (1:3 v/v) and the mixture was incubated overnight at 4°C. To recover the precipitate formed during storage centrifugation at 5000×g for 20 min was carried out. The precipitate was further dissolved in ultrapure water and extensively dialyzed (VISKING®, SERVA Electrophoresis, Germany, MWCO 12-14 kDa) against distilled water for 72 h at 4ºC, with a periodic change of water. The dialyzed EPSs were centrifuged at room temperature, ltered through a Büchner funnel, and freeze-dried. The obtained EPSs were named CO-EPS (C. ongulense) and LY-EPS (L. yakuticum).

Rusinova-Videva S., Ognyanov M., Georgiev Y., Petrova A., Dimitrova P., Dimitrova P, & Kambourova M. (2022). Chemical characterization and biological effect of exopolysaccharides synthesized by Antarctic yeasts Cystobasidium ongulense AL₁₀₁ and Leucosporidium yakuticum AL₁₀₂ on murine innate immune cells. World journal of microbiology & biotechnology, 39(2).

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