Microm hm360

Manufactured by Thermo Fisher Scientific

Sourced in United States, Germany

The Microm HM360 is a manual rotary microtome designed for sectioning paraffin-embedded tissue samples. It features a precision feed and stable specimen holder for producing high-quality tissue sections. The Microm HM360 is a compact and durable instrument suitable for routine histological applications in clinical and research laboratories.

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Lab products found in correlation

Eclipse 80i microscope, by Nikon (2 mentions) Tissue tek vip, by Sakura Finetek (2 mentions) Axio imager a1, by Zeiss (1 mentions) Leica dm 2500 led microscope, by Leica Microsystems (1 mentions) Dfc7000t, by Leica camera (1 mentions) Neutral buffered formalin, by Merck Group (1 mentions) Axioplan microscope, by Zeiss (1 mentions) Axiocam mrc5 camera, by Zeiss (1 mentions) Ds ri1 camera, by Nikon (1 mentions) Toluidine blue, by Merck Group (1 mentions) Dm4500, by Leica camera (1 mentions) Entellan new mounting medium, by Merck Group (1 mentions) Axiovision 4, by Zeiss (1 mentions) Rotipuran, by Carl Roth (1 mentions) Axiocam mrc camera, by Zeiss (1 mentions) Bx60 microscope, by Olympus (1 mentions) Hematoxylin, by Merck Group (1 mentions) Cellsafe biopsy capsules, by CellPath (1 mentions) Histogel, by Thermo Fisher Scientific (1 mentions) Tcs sp8, by Leica camera (1 mentions)

13 protocols using microm hm360

Histopathological Analysis of Organ Tissues

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The samples of the tissue fragments of each organ were excised, sliced, and immediately fixed with a 4% PFA for 1 h. The samples were dehydrated through a progressive series of ethanol, infiltrated by xylene, and embedded in paraffin. Serial sections (7.0 ± 0.5 μm) were prepared using a Microm HM-360 rotary microtome (Thermo Fisher Scientific, Dreieich, Germany), stained with hematoxylin and eosin solution, and then mounted. The mounted sections were observed under a microscope (Axio Imager A1, Zeiss). The histopathological state of the organs was estimated based on the work previously published by the authors [6 (link)].
Further quantitative analysis was performed only on the digestive gland and nephridia, based on their higher susceptibility to microalgae infection and severe tissue damage. A quantitative evaluation of histopathological changes in the organs was performed using the following parameters: karyopyknosis, hypervacuolisation, necrosis, hemocytic infiltration, fibrosis, and invasions of digestive gland and nephridia; thickness of the basement membrane, hyperplasia, and architecture (shape) of nephrocytes; the number, size, shape, and structure (density) of concretions in the nephridium tubules; and the ratio of tubule types, the granulocytomas, and fibromas of the digestive gland, as previously described by Kumeiko and colleagues [32 (link)].

Tumas A.V., Slatvinskaya V.A., Kumeiko V.V, & Sokolnikova Y.N. (2024). Study of the Impact of the Parasitic Microalgae Coccomyxa parasitica on the Health of Bivalve Modiolus kurilensis. Microorganisms, 12(5), 997.

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Histological Evaluation of Skin Damage

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Induction of skin damage and inflammation was evaluated by histology of intact skin, damage skin, and skin treated with SLS or MC903 in the pilot study. Abdominal skin tissue was fixed in 10% neutral buffered formalin (Hounisen, Skanderborg, Denmark) for 24 h. Tissues were dehydrated through increasing ethanol series (70–99%), rendered permeable with xylene, and embedded in paraffin. Tissues were cut in 5–6 μm sections using a microtome Microm HM 360 (Thermo Scientific, MA). All sections were stained with Haematoxylin–Eosin (HE; Ampliqon, Odense, Denmark). Slides were analysed using a Leica DM2500 LED microscope with the Leica Application Suite X version 5 software package (Leica Microsystems CMS GmbH Mannheim, Germany).

Larsen J.M., Ballegaard A.R., Dominguez A.S., Kristoffersen N.J., Maryniak N.Z., Locke A.V., Kazemi S., Epstein M., Madsen C.B, & Bøgh K.L. (2022). The role of skin inflammation, barrier dysfunction, and oral tolerance in skin sensitization to gluten‐derived hydrolysates in a rat model. Contact Dermatitis, 88(2), 109-119.

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Floral Bud Ultrastructure Visualization

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Flower buds of different lengths were vacuum-infiltrated and fixed with 2.5% (w/v) glutaraldehyde in 0.1 M phosphate buffer (pH 7.2). Fixed materials were dehydrated through a graded series of ethanol (50%, 70%, 80%, 90%, 100%, and 100%) and embedded in resin using a Technovit Embedding Kit (Germany). Semi-thin (2 μm) sections were obtained using an automatic microtome (Microm HM 360, Thermo, Waltham, MA, USA), stained with 1% toluidine blue for 10 s at 22 °C, and observed under a Nikon Eclipse 80i microscope (Nikon, Tokyo, Japan).

Xiao Q., Wang H., Chen H., Chen X., Wen J., Dai C., Ma C., Tu J., Shen J., Fu T, & Yi B. (2021). Molecular Analysis Uncovers the Mechanism of Fertility Restoration in Temperature-Sensitive Polima Cytoplasmic Male-Sterile Brassica napus. International Journal of Molecular Sciences, 22(22), 12450.

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In Situ Hybridization of Insulin Receptor Isoforms

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Organs were fixed in 10% neutral buffered formalin (Sigma). After 24 hours for liver, pancreas and brain, and 48 hours for pGAT, the tissues were placed in 70% ethanol prior to processing using a Tissue‐Tek VIP (Sakura Finetek, Alphen aan den Rijn, Nederland). Tissues were embedded in paraffin and cut into 4 μm sections using a microtome (Microm HM360, ThermoFisher). The sections were used for in situ hybridization using Base Scope^TM (ACDBio, Newark, CA, USA) paired double‐Z oligonucleotide probes. The IR‐B probe (BA‐Mm‐Insr‐tv2‐E11E12) targeting 2735‐2771 of NM 001330056.1 was visualized with red colour amplification. The IR‐A probe (BA‐Mm‐Insr‐tv1‐E10E11) targeting 2700‐2735 of NM 010568.3 was visualized in green. Negative (bacterial dapb) and positive (mouse ppib and polr2a) control probes were tested. Negative controls showed an almost negligible background (Figure S5B,D,E). Images were taken using a Leica SP8 equipped with a colour camera DFC7000T (Leica). Images were collected in Z‐stacks with 1.5 μm thickness. For each section, 1‐3 images were acquired using the navigator system on the LASX software, with maximum pixel resolution and using a 20× immersion objective. By using Fiji,¹⁶ each image was searched for red and green dots by eye and each dot was annotated using the Fiji cell counter plugin.

Moruzzi N., Lazzeri‐Barcelo F., Valladolid‐Acebes I., Moede T., Paschen M., Leibiger B., Berggren P, & Leibiger I.B. (2021). Tissue‐specific expression of insulin receptor isoforms in obesity/type 2 diabetes mouse models. Journal of Cellular and Molecular Medicine, 25(10), 4800-4813.

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Histological Assessment of Aortic Media

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Serial cross-sections (7 μm, 10 series) were made after paraffin embedding (Microm HM360; Thermo Scientific, Walldorf, Germany). Hematoxylin and eosin as well as Verhoeff–Van Gieson (VVG) staining were performed. Images of the ascending aorta were obtained at the level of the sinotubular junction at ×40 magnification using a Zeiss Axioplan microscope and an Axiocam MRc5 camera. Two blinded observers (D.V. and M.C.) performed measurements using ImageJ software and results were averaged. Media thickness, the average of 8 measurements (2 per quadrant), was obtained using hematoxylin and eosin–stained samples. VVG was used to evaluate wall architecture at 4 different locations, using an arbitrary scale based on the number of elastin breaks per cross-section. A “break” was defined as an isolated area where an elastic fiber was fragmented with interposed excessive connective tissue matrix. A scale of (1) indicated no breaks, (2) 1 to 2 breaks, (3) 3 to 4 breaks, (4) 5 to 6 breaks, and (5) 7 or more breaks.^{12 (link)} Dividing the area of elastic fibers by the total area of the media determined the elastin fiber content.^{22 (link)}

Verbrugghe P., Verhoeven J., Clijsters M., Vervoort D., Schepens J., Meuris B, & Herijgers P. (2018). The Effect of a Nonpeptide Angiotensin II Type 2 Receptor Agonist, Compound 21, on Aortic Aneurysm Growth in a Mouse Model of Marfan Syndrome. Journal of Cardiovascular Pharmacology, 71(4), 215-222.

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Histological Examination of Flower Buds

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Sterile and fertile flower buds were fixed and embedded in resin using Technovit Embedding Kits (Germany). Then, semi-thin (2.5 μm) sections were obtained using an automatic microtome (Microm HM 360, Thermo). The sections were stained with 0.1% toluidine blue O for 30–60 sec at room temperature and observed with a Nikon Eclipse 80i microscope (Nikon, Japan). Images of the anthers in different stages were captured with a Nikon DS-Ri1 camera (Nikon, Japan).

An H., Yang Z., Yi B., Wen J., Shen J., Tu J., Ma C, & Fu T. (2014). Comparative transcript profiling of the fertile and sterile flower buds of pol CMS in B. napus. BMC Genomics, 15, 258.

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Prostate Tissue Analysis: Verhoeff-Van Gieson and Toluidine Blue

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Prostate tissue was isolated, fixed overnight in 10 % buffered formalin, and paraffin sections (3 μm) cut on a motorised microtome (Microm HM360, ThermoFisher). Sections were stained with Verhoeff van Gieson for elastin/collagen (HT25A, Millipore-Sigma, Burlington, MA, USA) or acidified 1% Toluidine blue for mast cells. Images were recorded as bright-field 3D montages (Fluoview 3000/CellSense software, ≤15×15 frames, Olympus Corp, Center Valley, PA, USA) and analysed using NDP and HCImage software (Hamamatsu Photonics, Bridgewater, NJ, USA).

Zabbarova I.V., Ikeda Y., Kozlowski M.G., Tyagi P., Birder L., Chakrabarty B., Perera S., Dhir R., Straub A.C., Sandner P., Andersson K.E., Drake M., Fry C.H, & Kanai A. (2022). Benign prostatic hyperplasia/obstruction ameliorated using a soluble guanylate cyclase activator. The Journal of pathology, 256(4), 442-454.

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Fixation, Sectioning, and Microscopy of Tissues

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Sectioning, staining and microscopic observations were carried out as described by Tsuge et al. (1996) (link). Briefly, tissue samples were fixed with formalin-acetic acid-alcohol solution and dehydrated with ethanol series and embedded in Technovit 7100 resin (Kulzer and Co., Wehrheim, Germany), followed by 10-12 μm-thickness sectioning with a microtome (Microm HM360, Thermo Fischer Scientific, USA). Sections were stained with 0.1% (w/v) toluidine blue (Sigma) in 0.1 M phosphate buffer (pH 7.0), washed with water, dried, and mounted on glass slides with Entellan new mounting medium (EMD, Millipore, Burlington, MA, USA). Microphotography was done using a differential interference contrast microscope (DM4500, Leica, Germany).

Tsukaya H, & Ohtake Y. (2023). Cavity and entrance pore development in ant plant hypocotyls. Frontiers in Plant Science, 14, 1234650.

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Histological Analysis of Komatiid Beetle Anatomy

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For histological sectioning, we used two males of K. biedouwense and one male of V. clanwilliamense and K. botterkloofense, respectively. The abdomens were cut off, dehydrated in an ascending series of ethanol and then embedded into paraffin using three intermediate steps with tetrahydrofuran (Rotipuran®, Carl Roth GmbH + Co. KG, Germany) to facilitate penetration: 2 h 1:1 in ethanol, 24 h pure tetrahydrofuran, and 24 h 1:1 in paraffin [15 (link)]. Sections (5 μm thick) were made with a Microm HM 360 rotary microtome (Thermo Fisher Scientific, MA, USA), stained with Azan-novum according to Geidies [26 ], and analysed using an Olympus BX60 microscope (Olympus, Japan) equipped with a Zeiss AxioCam MRc camera (Carl Zeiss AG, Germany), and AxioVision 4.8 software (Carl Zeiss AG, Germany).
If not stated otherwise, our morphological terminology follows Klass et al. [17 ] for postabdominal structures and Snodgrass [12 ] for internal genitalia. All figure plates were arranged using Corel Draw (X7, Corel Corporation). Measurements were conducted on the histological sections using the AxioVision 4.8 software (note that measurements were done on the fixed, embedded and sectioned structures).

Kreuz J, & Eberhard M.J. (2022). Asymmetry of the male internal reproductive organs in Mantophasmatodea. BMC Zoology, 7, 4.

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Feather Melanosomes Imaging and Analysis

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Feather follicles pretreated with paraformaldehyde (PFA) as described above were dehydrated in an ethanol serial dilution of 70%, 95%, and 99.9%, and then embedded in Technovit 7100 (EMS, USA). Serial plastic cross-sections of 3 μm in thickness were obtained using a microtome (MICROM HM 360, ThermoFisher Scientific Inc., USA), and subsequently stained with hematoxylin (Merck & Co. Inc., USA) and eosin (BDH Chemicals, UK). To identify the morphologic features of melanosomes, PFA-fixed feathers were dissected and squeezed, and then wet-mounted with PBS. Bright-field histological or whole-mounted images were taken using a Leica Imager M2 equipped with a Hamamatsu digital camera C1440.

Wu C.C., Klaesson A., Buskas J., Ranefall P., Mirzazadeh R., Söderberg O, & Wolf J.B. (2019). In situ quantification of individual mRNA transcripts in melanocytes discloses gene regulation of relevance to speciation. The Journal of experimental biology, 222(Pt 5), jeb194431.

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