H9c2 cells

Manufactured by Selleck Chemicals

Sourced in China

H9C2 cells are a clonal line of myoblasts derived from rat heart tissue. They are commonly used as an in vitro model for the study of cardiac muscle cell biology and function.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Dulbecco s modified eagle medium, by Thermo Fisher Scientific (2 mentions) Dronedarone, by Selleck Chemicals (1 mentions) Ang 2, by Selleck Chemicals (1 mentions) H9c2 cell, by American Type Culture Collection (1 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions) Sodium palmitate, by Merck Group (1 mentions) H9c2 cells, by Shanghai Institute of Biochemistry and Cell Biology (1 mentions)

2 protocols using h9c2 cells

Ang II-induced H9C2 cell model of MH

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H9C2 cells were bought from ATCC (Rockville, MD, USA). All cells were maintained in Dulbecco’s Modified Eagle medium (Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal bovine serum (Thermo Fisher Scientific) and 1% antibiotics (Beyotime, Shanghai, China) under the condition of 5% CO₂ and 37°C.
H9C2 cells were treated with 50, 100, 150 nmol/L Ang II (BIOFOUNT, Beijing, China) for 48 hours to establish a cell model of MH.
In addition, different concentrations of dronedarone (1, 5, 10 μM) (Selleck, Shanghai, China) were used to treat Ang II-induced H9C2 cells for 6 hours.

Chen C., Hu S., Hu H.J., Liu Z.X., Wu X.T., Zou T, & Su H. (2024). Dronedarone Attenuates Ang II-Induced Myocardial Hypertrophy Through Regulating SIRT1/FOXO3/PKIA Axis. Korean Circulation Journal, 54(4), 172-186.

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Palmitate-Induced Metabolic Stress in H9C2 Cells

Cited 1 time

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The heart myoblast cell line (H9C2 cells) was obtained from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China) and was cultured in high glucose Dulbecco's modified Eagle's medium (DMEM; Gibco, Rockville, MD, USA), containing 10% fetal bovine serum (Gibco). Increased levels of plasma nonesterified fatty acids (NEFAs) occur in both obesity and T2DM, and PA is the most abundant plasma saturated fatty acid [24 (link)]. To study the effects of EMPA on the elevated levels of NEFAs that occur in obesity and T2DM, H9C2 cells were exposed to PA to mimic the cellular metabolic environment of obesity and type 2 diabetes. PA stock solution (10 mM) was prepared by dissolving sodium palmitate (Sigma-Aldrich, St. Louis, MO, USA) in 50% ethanol solution mixed with 2% fatty acid-free bovine serum albumin (BSA) solution, as previously described [25 (link)]. H9C2 cells were exposed to 200 μM PA under a range of concentrations of EMPA (Selleck, Houston, TX).

Wang J., Huang X., Liu H., Chen Y., Li P., Liu L., Li J., Ren Y., Huang J., Xiong E., Tian Z, & Dai X. (2022). Empagliflozin Ameliorates Diabetic Cardiomyopathy via Attenuating Oxidative Stress and Improving Mitochondrial Function. Oxidative Medicine and Cellular Longevity, 2022, 1122494.

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