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3 protocols using tyrosine

1

Microcrystalline Cellulose and Casein-based Formulation

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Microcrystalline cellulose (MCC) (Avicel PH 102), dicalcium phosphate dihydrate (DCP), magnesium stearate, casein, tyrosine, and trichloroacetic acid (98.0%) were purchased from HiMedia Laboratories Pvt. Ltd., Mumbai, India. Potassium dihydrogen phosphate, sodium hydroxide (Qualigens Fine Chemicals, Mumbai, India) and papain (source Carica papaya), disodium ethylenediaminetetraacetate (99.5%), cysteine hydrochloride (99.0%), carrageenan, tragacanth, agar, and citric acid (98.0%) were purchased from S. D. Fine-Chem Ltd., Mumbai, India. All chemicals were used as received. Double-distilled water was used throughout the study.
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2

Enzyme Assay Protocol for Polyphenol Oxidase

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Guaiacol, 2, 2′-azino bis[3-ethylbenzthiazoline-6-sulfonate] (ABTS), syringaldazine (SGZ), sephadex G-150 and DEAE-cellulose were purchased from Sigma (USA). Other substrates viz. pyrogallol, α-naphthol, p-phenylenediamine (PPD), resorcinol, 2, 6-dimethoxyphenol (DMP), catechol, 3,4-dihydroxy-phenylalanine (L-DOPA) and tyrosine were purchased from Hi-media. Other chemicals used were of analytical grade.
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3

Protein Extraction and Purification

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Casein, EDTA, TEMED, Bradford reagent, and α-amylase enzyme (Aspergillus oryzae) were purchased from Merck; starch, tyrosine, prestained protein ladder, DEAE Sepharose, and TCA were purchased from HiMedia Laboratories. Bromophenol blue was obtained from Genei and DTT from SERVA Electrophoresis GmbH, Heidelberg, Germany. All chemicals and reagents were of analytical grade.
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