Jsm t200

The interaction between carp glycophorin and V. anguillarum was examined by titrating the glycophorin on a glass cover slip treated with poly-l-lysine. After 20 min, the cover slip was dried in liquid carbon dioxide using a Hitachi HCP-2 critical-point drying apparatus (Hitachi Ltd., Tokyo, Japan). The cover slip was coated with platinum-palladium in a JEOL JFC-1100 ion-sputtering apparatus (JEOL) and observed under a JEOL JSM-T200 (SEM) operated at 10 kV.
The interaction of the carp glycophorin and M. luteus was observed by a carbon rod (5 mmØ carbon rod, JEOL) on a cover slip, which was treated with poly-l-lysine. After 40 min, the carbon plate was fixed with osmium tetroxide and stained to prevent the accumulation of a charge with tannic acid. The fixed carbon plate was dried in liquid carbon dioxide using a Hitachi HCP-2 critical-point drying apparatus. The carbon plate was then coated with platinum-palladium in a JEOL JFC-1100 ion-sputtering apparatus and observed under a Hitachi S-4000 (SEM).
The glycophorin was negatively dyed using 2% phosphotungstic acid (pH 7.0) and observed under a Hitachi H-800 (TEM).

Four barley cultivars (Giza 123, Giza 126, Giza 136, and Giza 138) were studied using SEM. Those four cultivars were chosen based on the recommendations of the plant breeders. The chosen cultivars have high production demand and can withstand harsh conditions; they also have excellent synthetic qualities, which is the reason for their examination. For example, Giza 123 tolerates harsh conditions and high salinity levels; Giza 126 has excellent drought tolerance, and is grown under the rain on the northern coast of Egypt, while Giza 136 and Giza 138 are characterized by high yield under all conditions. Viable grains of the studied cultivars were obtained during the season of 2019. The clean and dry seed samples of the studied barley cultivars were placed on double-stick tape mounted on a copper electron microscope holder. The specimens were coated with gold, and then investigated and photographed with a JEOL JSM T200 at 25 kV, in the electron microscope unit of Mansoura University, Mansoura, Egypt. Seed coat technical terms were based on the works of Koul, et al. [71 (link)],Murley [72 (link)],and Stearn [73 ].