Flatbed scanner

Manufactured by Hewlett-Packard

Sourced in Canada

The Flatbed scanner is a device that captures digital images of physical documents, photographs, or other flat objects. It uses a light source and a sensor array to scan the object and convert it into a digital file that can be stored on a computer or shared electronically.

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Lab products found in correlation

Collagen gel, by Cell Biolabs (1 mentions) Flatbed scanner, by Adobe (1 mentions) Diamidino 2 phenylindole dapi, by Thermo Fisher Scientific (1 mentions) Optimal cutting temperature compound oct, by Thermo Fisher Scientific (1 mentions) Objective lens, by Zeiss (1 mentions) Tissuefaxs system, by TissueGnostics (1 mentions)

7 protocols using flatbed scanner

Quantifying Depigmentation in Mice

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From 5 weeks to 15 weeks of age, mice were scanned weekly on a flatbed scanner (Hewlett-Packard, Palo Alto, CA) under isoflurane anesthesia. Using Adobe Photoshop software (Adobe Systems) ventral and dorsal luminosity was measured to calculate depigmentation, as previously described (47 (link)). Depigmentation was graphed over time, and statistical significance was determined by the time-adjusted area under the curve (AUC). Representing change in depigmentation from treatment initiation was calculated using the trapezoidal rule. No imputation was done for missing data, and the AUC for each mouse was divided by the total number of weeks of available data minus 1. The Wilcoxon Rank Sum (WRS) test was used to compare the time-adjusted AUC among groups.

Mukhatayev Z., Dellacecca E.R., Cosgrove C., Shivde R., Jaishankar D., Pontarolo-Maag K., Eby J.M., Henning S.W., Ostapchuk Y.O., Cedercreutz K., Issanov A., Mehrotra S., Overbeck A., Junghans R.P., Leventhal J.R, & Le Poole I.C. (2020). Antigen Specificity Enhances Disease Control by Tregs in Vitiligo. Frontiers in Immunology, 11, 581433.

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Collagen Gel Contraction Assay

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hTERT-HM^Tet-3G cells (2.5 × 10⁵) cultured overnight in phenol red free DMEM/F12 complete growth medium were infected with ERΔ7 lentiviral particles in the presence or absence of Dox (500 ng/ml). After 48 h, cells were trypsinized and seeded into collagen gel and contraction assay was carried out following the manufacturer's protocol (Cell Biolabs Inc. San Diego, CA, USA). In brief, collagen lattice was prepared by mixing 2 parts of cell suspension and 8 parts of cold collagen gel solution to achieve 1.5 × 10⁵ cells/well. 0.5 mL of the cell-collagen mixture was added per well in a 24-well plate and incubated for 1 h at 37 °C to allow gelling. 1.0 mL of phenol-red free DMEM/F12 containing 2% charcoal-stripped FBS, 1% antibiotic-antimycotic, supplemented with or without Dox (500 ng/mL) and E2 (100 nM) was added over the cell-collagen matrix. The gels were gently released after 20 h and the area of the lattices were measured periodically from 4 to 36 h. Images of the floating gels were captured and digitized using a flatbed scanner (Hewlett Packard) and the mean gel area (cm²) was measured using IMAGEJ software. No Dox treatment served as control.

Anamthathmakula P., Kyathanahalli C., Ingles J., Hassan S.S., Condon J.C, & Jeyasuria P. (2018). Estrogen receptor alpha isoform ERdelta7 in myometrium modulates uterine quiescence during pregnancy. EBioMedicine, 39, 520-530.

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Quantifying Depigmentation in Mice

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Depigmentation was documented at 5 week age intervals using a flatbed scanner (Hewlett-Packard Company, Palo Alto, CA) and Adobe Software (Adobe Systems, Inc., San Jose, CA). Depigmentation was calculated as described (Denman et al., 2008 (link)). Briefly, anesthetized mice were placed on a flatbed scanner and resulting images were subjected to image analysis using Adobe Photoshop. Depigmentation was calculated from the largest evaluable area as the percentage of pixels among >150,000 evaluated with a luminosity above the cutoff level set to include 95% of pixels for untreated mice. Different animals were used to quantify depigmentation at each time point with the exception of Vitesse mice beyond 30 weeks of age. These later time points were included to evaluate when depigmentation tapers off in both groups. As relevant, mice were photographed using a DSC-S950 SteadyShot digital camera (Sony, Tokyo, Japan).

Eby J.M., Kang H.K., Klarquist J., Chatterjee S., Mosenson J.A., Nishimura M.I., Garrett-Mayer E., Longley B.J., Engelhard V.H., Mehrotra S, & Le Poole I.C. (2014). Immune Responses in A Mouse Model of Vitiligo with Spontaneous Epidermal De- and Repigmentation. Pigment cell & melanoma research, 27(6), 1075-1085.

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Quantifying Myocardial Infarction and Inflammation

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Infarct size measurement: Excised hearts were sectioned axially into 1 mm thickness (McIlwain 800 Series, Vibratome, St Louis, MO) and stained with 2,3,5-triphenyltetrazolium chloride (TTC, from GFS Chemicals, Powell, OH) following the manufacturer's protocol. TTC images were acquired on a flatbed scanner (Hewlett Packard, Palo Alto, CA), and microsphere images were acquired on the IVIS spectrum. Image analysis was performed in ImageJ, with infarction size across the heart slices normalized to the area-at-risk (AAR).
Immunohistochemistry: Excised hearts were embedded in Optimal Cutting Temperature compound (OCT, from Thermo Fisher), and 10 μm short-axis cryo-sections were collected. Direct immunohistochemistry of CD68 was performed with anti-CD68 antibody conjugated to Alexa Fluor 647 fluorophore (Santa Cruz Biotechnology, Dallas, TX) per manufacturer's instructions. Diamidino-2-phenylindole (DAPI, from Thermo Fisher) staining was performed to visualize the nuclei. Microscopy was performed on a TISSUEFAXS system (TissueGnostics GmbH, Vienna, Austria), with a 40X objective lens (Zeiss), DAPI (for DAPI), GFP (for Gd-TO), and Alexa 647 (for CD68) filter settings. Images were acquired on a 12 bit CCD camera (Pixelink, Ottawa, Ontario, Canada), with the TissueFAXS image acquisition software, and analyzed in Image J (NIH).

Chen H.H., Yuan H., Cho H., Feng Y., Ngoy S., Kumar A.T., Liao R., Chao W., Josephson L, & Sosnovik D.E. (2017). Theranostic Nucleic Acid Binding Nanoprobe Exerts Anti-inflammatory and Cytoprotective Effects in Ischemic Injury. Theranostics, 7(4), 814-825.

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Quantifying Depigmentation in Mice

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Depigmentation was measured in mice receiving Ccl22 encoding DNA or vector only, starting 2 weeks after the final treatment as hair returned. Depigmentation was followed for 6 weeks. Mice were placed on a flatbed scanner (Hewlett-Packard Company, Palo Alto, CA) and the ventral and dorsal sides of the mice were imaged. The images were captured in Adobe Photoshop Software (Adobe Systems, Inc, San Jose, CA). Histograms were prepared essentially as described, displaying mice before treatment to capture a cutoff point incorporating 95% of pixels to reflect pigmented pelage (to allow for small areas not covered by pigmented hair). The increase in the proportion of pixels crossing the cutoff value reflects the % depigmentation of mice after treatment (Denman et al., 2008 (link)).

Eby J.M., Kang H.K., Tully S.T., Bindeman W.E., Peiffer D.S., Chatterjee S., Mehrotra S, & Le Poole I.C. (2015). CCL22 to activate Treg migration and suppress depigmentation in vitiligo. The Journal of investigative dermatology, 135(6), 1574-1580.

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Quantifying Depigmentation in Mice

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Skin Prick Test Wheal Measurement Protocol

Cited 1 time

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The outcome of the SPT can result in a variety of wheal shapes, as shown in Fig. 1. To determine the average diameter, the mean value of the longest and the midpoint orthogonal diameter (mm) of the wheal were measured (Fig. 2). The area of the wheal was determined by using a flatbed scanner (Hewlett Packard) in combination with software earlier developed by Erasmus MC: Precise Automated Area Measurement of Skin Test (PAAMOST) [6 , 7 (link)]. Mean values of two histamine-induced wheal sizes of the positive control were collected as well. Based on the measured data the HEP-indices were calculated for both the average diameter and area.Fig. 1

Typical observed wheal forms in SPT’s

Fig. 2

Definition of D1 and D2

Consequently the four readings were defined as:

Average diameter (allergen-induced average wheal diameter).

Area (allergen-induced area measured by scanning device).

HEP-index diameter (allergen-induced average diameter divided by histamine-induced average diameter).

HEP-index area (allergen-induced area divided by the histamine-induced average area).

van der Valk J.P., Gerth van Wijk R., Hoorn E., Groenendijk L., Groenendijk I.M, & de Jong N.W. (2016). Measurement and interpretation of skin prick test results. Clinical and Translational Allergy, 6, 8.

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