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5 protocols using sodium tellurite

1

Synthesis and Characterization of Nanoparticles

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Thioglycolic acid (TGA, C2H4O2S, ≥99%), cadmium acetate dihydrate (Cd(CH3COO)2·2H2O, 99.5%), (3-aminopropyl)triethoxysilane (APTES, H2N(CH2)3Si(OC2H5)3, 99%), tetraethyl orthosilicate (TEOS, SiC8H20O4, 98%), ammonia (NH3, ≥99.9%), cetyltrimethylammonium bromide (CTAB, C19H42BrN, ≥98%), poly(ethylene glycol) BioUltra 3000, poly(ethylene glycol) BioUltra 6000, poly(ethylene glycol) BioUltra 8000, p-toluenesulfonyl chloride (CH3C6H4SO2Cl, ≥98%), hydrochloric acid (HCl, 37%), magnesium sulfate (MgSO4, ≥99.5%), sodium ascorbate, bovine serum albumin (BSA), lysozyme (LYS), carbonic anhydrase (CA), ovalbumin (OVA), hemoglobin (Hb), myoglobin (Myb), and cytochrome c (CYT) were purchased from Sigma-Aldrich (Lisbon, Portugal). Sodium hydroxide (NaOH, ≥98%) was produced by Panreac. Sodium tellurite (Na2TeO3, 99.5%) and ammonium nitrate (NH4NO3, 99.9%) were purchased from Alfa Aesar. Sodium borohydride (NaBH4, 99%), ethylene glycol (C2H6O2, ≥99.5%) and (3-isocyanatopropyl)triethoxysilane (IPTES, (C2H5O)3Si(CH2)3NCO, 95%) were produced by Fluka. Methanol (MeOH), ethanol (EtOH), tetrahydrofuran (THF, C4H8O, ≥99.9%), and chloroform (CHCl3) were purchased by Carlo Erba Reagents. All the reagents and solvents were of analytical reagent grade and were used as received. All cell culture reagents were purchased from Gibco (Alfagene, Lisbon, Portugal) unless stated otherwise.
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Nanoparticle Synthesis and Antibacterial Assay

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Cadmium chloride (CdCl2), 3-mercaptoundecanoic acid (MPA), sodium citrate dihydrate sodium borohydride (Na2BH4), l-ethyl-3-(3-(dimethylamino)propyl) carbodiimide (EDC), Triton X-100, 2′,7′-dichlorofluorescein (H2DCF), sodium phosphate monohydrate, AlamarBlue and melittin were obtained from Sigma-Aldrich. Sodium hydroxide (NaOH) was obtained from Acros Organic. Sodium tellurite was obtained from Alfa Aeser. Tris buffer was obtained from AppliChem. Aqua (sterile water) was obtained from BBraun. Mueller Hinton Broth (MHB) was obtained from BD Difco. EDTA-blood was either drawn fresh every day of assay from an approved volunteer or purchased from Innovative Research, Inc. (Michigan, U.S.). E. coli (Strain ATCC25922) cells were purchased from the Leibniz-Institute DSMZ. Poly(oxanorbornenes) (PONs) were synthesized and characterized as reported previously (see SI for details).21 (link),23 (link) An Ultrathin LED Light Panel (Neutral White) was purchased from Environmental Lights, San Diego, CA.
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3

Synthesis of Bismuth Telluride Nanostructures

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Sodium tellurite (NaTeO3, >99.99%), bismuth nitrate pentahydrate (Bi(NO3)3·5H2O, >90%), sodium hydroxide (NaOH, >90%), poly(vinyl pyrrolidone) (PVP, MW ≈ 40 000) and ethylene glycol (EG) were all purchased from Alfa Aesar. Ethylenediamine (C2H8N2) (99%) was obtained from Acros. Graphite powder (325 mesh), potassium permanganate (KMnO4, >90%), concentrated sulfuric acid (H2SO4, 98%), hydrogen peroxide (H2O2, 30%), hydrochloric acid (HCl, 37%), anhydrous acetone and isopropanol were supplied by Sinopharm. All the chemicals were used as received without further purification.
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Synthesis of Tellurium Nanomaterials

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Bismuth nitrate pentahydrate (Bi(NO 3 ) 3 H 2 O, >99.9%), sodium tellurite (Na 2 TeO 3 , >99.5%), sodium hydroxide (NaOH, >99%), poly(vinyl) pyrrolidone (PVP, MW 40 000) and ethylene glycol were purchased from Fisher Scientific. All chemicals were used as received.
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5

Synthesis of Bismuth Chalcogenide Nanostructures

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Chemicals and solvents: Bismuth (III) nitrate pentahydrate (Bi(NO3)3•5H2O, ≥ 99.99%), sodium selenite (Na2SeO3, ≥ 98%), potassium hydroxide (KOH, ≥ 98%), and polyvinylpyrrolidone (PVP, (C6H9NO)n, average mol wt ~55,000) were purchased from Sigma Aldrich. Sodium tellurite (Na2TeO3, 99.5%) and ethylene glycol (EG, HOCH2CH2OH, 99%) were purchased from Fisher.
Analytical grade acetone and ethanol were obtained from various sources. All chemicals were used as received without further purification. Syntheses were carried out using a standard vacuum/dry argon Schlenk line.
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