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Intracellular samhd1

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SAMHD1 is a human protein that plays a role in regulating cellular nucleotide levels. It is involved in the metabolism of deoxyribonucleotides, which are the building blocks of DNA. SAMHD1 functions as a deoxyribonucleoside triphosphate triphosphohydrolase, catalyzing the conversion of deoxyribonucleotides to their corresponding nucleosides and inorganic triphosphate.

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Lab products found in correlation

Ethylene diamine tetraacetic acid (edta), by Thermo Fisher Scientific (2 mentions) Sodium azide, by Merck Group (2 mentions) Cyan adp analyzer, by Beckman Coulter (2 mentions) Cd133, by BD (2 mentions)

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SAMHD1 (5 citations)

2 protocols using intracellular samhd1

1

Characterization of Hematopoietic Stem and Progenitor Cells

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HSPCs and monocytes were incubated with PBS containing 2% FBS, 2 mM EDTA (Invitrogen Life Technologies) and 0.1% sodium azide (Sigma-Aldrich) for staining cell surface markers. Intracellular SAMHD1 (Abcam, Cambridge, UK) staining was performed as described (Baldauf et al., 2012 (link)). Depending on the purpose of the experiment, the following antibodies were used for staining HSPCs in various combinations: Lin-, CD34, CD133, and CD38 (BD Biosciences, San Jose). Samples were acquired on a CyAn™ ADP Analyzer (Beckman Coulter, Pasadena, CA) and analyzed by FlowJo software (TreeStar, Ashland, OR). Gating strategy is shown in Fig. 2A.
Li D., Schlaepfer E., Audigé A., Rochat M.A., Ivic S., Knowlton C.N., Kim B., Keppler O.T, & Speck R.F. (2015). Vpx mediated degradation of SAMHD1 has only a very limited effect on lentiviral transduction rate in ex vivo cultured HSPCs. Stem cell research, 15(2), 271-280.
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2

Characterization of Hematopoietic Stem and Progenitor Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
HSPCs and monocytes were incubated with PBS containing 2% FBS, 2 mM EDTA (Invitrogen Life Technologies) and 0.1% sodium azide (Sigma-Aldrich) for staining cell surface markers. Intracellular SAMHD1 (Abcam, Cambridge, UK) staining was performed as described (Baldauf et al., 2012 (link)). Depending on the purpose of the experiment, the following antibodies were used for staining HSPCs in various combinations: Lin-, CD34, CD133, and CD38 (BD Biosciences, San Jose). Samples were acquired on a CyAn™ ADP Analyzer (Beckman Coulter, Pasadena, CA) and analyzed by FlowJo software (TreeStar, Ashland, OR). Gating strategy is shown in Fig. 2A.
Li D., Schlaepfer E., Audigé A., Rochat M.A., Ivic S., Knowlton C.N., Kim B., Keppler O.T, & Speck R.F. (2015). Vpx mediated degradation of SAMHD1 has only a very limited effect on lentiviral transduction rate in ex vivo cultured HSPCs. Stem cell research, 15(2), 271-280.
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