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Terbium 3 chloride

Manufactured by Merck Group
Sourced in Spain

Terbium(III) chloride is an inorganic compound with the chemical formula TbCl3. It is a crystalline solid that is typically used as a source of terbium ions in various applications, such as in the production of luminescent materials and as a dopant in optical fibers. The compound has a molar mass of 240.12 g/mol and is soluble in water and other polar solvents.

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4 protocols using terbium 3 chloride

1

GSDMD Protein Detection Protocol

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Polyclonal anti-human GSDMD was from Novus Biologicals (NBP2-33422) or Proteintech (20770-1-AP). Monoclonal anti-haemagglutinin (F-7) antibody (sc-7392) was from Santa Cruz Biotechnology. Monoclonal anti-Flag M2 antibody (F1804), monoclonal anti-human α-tubulin antibody (T5168) and monoclonal anti-mouse caspase-11 antibody (C1354) were from Sigma-Aldrich. Polyclonal anti-human Na+/K+-ATPase α1 (ATP1A1) antibody (#3010) was from Cell Signaling. c-MYC (9E10) monoclonal antibody (MMS-15P) was from Covance. Monoclonal anti-human perforin antibody (3465-6-250) and polyclonal anti-human granulysin antibody (AF3138) were from Mabtech and Novus, respectively. N-ethylmaleimide, 2ME, DTT, terbium(III) chloride, DPA (dipicolinic acid) and nigericin were from Sigma-Aldrich. Ultrapure LPS and Pam3CSK4 were from InvivoGen. The complete protease inhibitor cocktail was from Roche. siRNA duplexes targeting Gsdmd (s87492; 5′-GGUGAACAUCGGAAAGAUUTT-3′) and the nonspecific control siRNA (CTL, 4390843) were from Ambion.
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2

Synthesis of Luminescent Nanoparticles

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Nanoparticles zinc oxide (<100 nm particles size), Erbium (III) chloride hexahydrate, Europium (III) chloride hexahydrate, Terbium (III) chloride, Dysprosium (III) chloride hexahydrate, Europium (III) chloride hexahydrate and Deionized (DI) water were purchased from Sigma Aldrich (Madrid, Spain), and used without any further purification.
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3

Synthesis of Metal-Organic Complexes

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Citric
acid, mercury(II)
acetate, cobalt(II) chloride hexahydrate, nickel(II) chloride hexahydrate,
cadmium(II) chloride, copper(II) chloride dihydrate, iron(II) sulfate
heptahydrate, manganese(II) acetate tetrahydrate, lead nitrate, zinc(II)
sulfate heptahydrate, sodium chloride, and sodium hydroxide were purchased
from Merck, India. p-Phenylenediamine was purchased
from Loba Chemie, and terbium(III) chloride and dialysis bags (1 kDa)
were purchased from Sigma-Aldrich. All reagents were of analytical
grade and used without further purification. Milli-Q water was used
for all the experiments.
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4

Biophysical Characterization of Protein Interactions

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Guanidine hydrochloride (GndHCl), thioflavin T (ThT), cholesterol (Chol), sodium cholate, ethylenediaminetetraacetic acid (EDTA), sodium chloride (NaCl), sodium dodecyl sulfate (SDS),Terbium -III chloride (Tb) and dipicolinic acid (DPA) were from Sigma-Aldrich (St Louis, MO); 1-palmitoyl-2-oleoylphosphatidylcholine (POPC) and dimyristoylphosphatidylcholine (DMPC) were purchased from Avanti Polar Lipids (Alabaster, AL); His-purifying resin was from Novagen (Darmstadt, Germany). Bis-(sulfosuccinimidyl) suberate (BS 3 ) and isopropyl-β-D-thiogalactoside (IPTG) were purchased to Thermo Scientific (Waltham, MA). All other reagents were of the highest analytical grade available.
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