Storage phosphor imaging plates

Unless otherwise stated, reagents were purchased from Fisher Scientific, Sigma-Aldrich, or Life Technologies. T4 polynucleotide kinase, molecular-biology-grade bovine serum albumin (BSA), and RNase inhibitor were purchased from New England BioLabs. [γ-³²P] ATP was purchased from PerkinElmer Life Sciences. Avian myeloblastosis virus (AMV) reverse transcriptase, Thermus filiformis (Tfi) DNA polymerase, and dNTP mix were purchased from Promega. The QuikChange Xl II mutagenesis kit was purchased from Agilent Technologies. RNA oligonucleotides were synthesized at the University of Utah DNA/Peptide Core Facility or purchased from Dharmacon. DNA oligonucleotides were purchased from Integrated DNA Technologies. Storage phosphor imaging plates from Molecular Dynamics were imaged with a Molecular Dynamics 9400 Typhoon phosphor imager. Data were analyzed with Molecular Dynamics ImageQuant 5.2 software. Matrix Assisted Laser Desorption/Ionization (MALDI) mass spectrometry of oligonucleotide samples was performed at the Campus Mass Spectrometry Facilities, UC Davis. Oligonucleotide masses were determined with Mongo Oligo Mass Calculator 2.08.

Unless otherwise stated, reagents were purchased from Fisher Scientific, Sigma-Aldrich, or Life Technologies. Brown Centrex columns were purchased from VWR. T4 polynucleotide kinase, T4 DNA ligase, molecular biology grade bovine serum albumin (BSA), chemically competent DH5α Escherichia coli and RNase inhibitor were purchased from New England Biolabs. γ-[³²P] ATP was purchased from Perkin-Elmer Life Sciences. The Avian Myeloblastosis Virus (AMV) reverse transcriptase, deoxynucleotide triphosphate (dNTP) mix and RQ1 RNase free DNase were purchased from Promega. Nuclease T1 and nuclease V1 were purchased from Ambion. Pfu Ultra II was purchased from Stratagene. Dpn 1 was purchased from Invitrogen. RNA oligonucleotides were synthesized at the University of Utah DNA/Peptide Core Facility or purchased from GE Healthcare Dharmacon, Inc. or Sigma Aldrich. DNA oligonucleotides were purchased from Integrated DNA Technologies or Sigma Aldrich. Storage phosphor imaging plates from Molecular Dynamics were imaged using Molecular Dynamics 9400 Typhoon phosphorimager. Data were analyzed using Molecular Dynamics ImageQuant 5.2 software. Electrospray Ionization (ESI) mass spectrometry of oligonucleotide samples was carried out at either the Campus Mass Spectrometry Facilities, UC Davis or at Novatia, LLC. Oligonucleotide masses were determined using Mongo Oligo Mass Calculator v2.06.