Mda mb 231

Manufactured by BNCC

Sourced in China

MDA-MB-231 is a human breast adenocarcinoma cell line. It is a commonly used model for the study of triple-negative breast cancer.

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Lab products found in correlation

Mcf 10a, by BNCC (12 mentions) Mcf 7, by BNCC (12 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (7 mentions) Mda mb 468, by BNCC (5 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (5 mentions) Mcf 10a, by Thermo Fisher Scientific (3 mentions) Mda mb 453, by BNCC (3 mentions) Mda mb 231, by Thermo Fisher Scientific (2 mentions) Mcf 7, by Thermo Fisher Scientific (2 mentions) Hcc1937, by BNCC (2 mentions) Sk br 3, by BNCC (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Rpmi 1640, by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by BNCC (2 mentions) Lip2000transfection reagent, by Biosharp (2 mentions) L 15 medium, by BNCC (2 mentions) Mir 18a 5p agomir, by GenePharma (2 mentions) Pcdna3, by GenePharma (2 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions)

12 protocols using mda mb 231

Profiling miR-18a-5p and HER2 in Breast Cancer

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To confirm the expression of miR−18a−5p and HER2 in BC cells and non-cancerous breast cells, human mammary epithelial cell line (MCF−10A) and human breast cancer cell lines (MDA-MB−231, MDA-MB−453, and MCF−7) were acquired from the BeNa Culture Collection (BNCC, China). MCF−10A cells were cultured in MEGM (BNCC), MCF−7 cells in DMEM (BNCC), while MDA-MB−453 and MDA-MB−231 cells were cultured in L−15 medium (BNCC). 10% fetal bovine serum (FBS; BNCC) was added to the culture medium and the cells were maintained at 37°C and 5% CO₂.
The agomiR NC, mimic negative control (NC), miR−18a−5p mimic, and miR−18a−5p agomiR were obtained from GenePharma (China). HER2 overexpression vectors and empty vectors were constructed using pcDNA3.1, obtained from GenePharma (China). Lip2000Transfection Reagent (Biosharp, China) was used to transfect the recombinant plasmids listed above into both MDA-MB−231 and MCF−7 cells. Cell transfection was performed for 48 h and assessed by qPCR analysis.

Liu Y, & Yang H. (2023). MiR-18a-5p attenuates HER2-positive breast cancer development by regulating PI3K/AKT pathway. Cancer Biology & Therapy, 24(1), 2224512.

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Hypoxia-Induced Breast Cancer Cell Regulation

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Two BC cell lines (MDA-MB-231 and MDA-MB-468) and breast epithelial cells (MCF-10A) were brought from BeNa Culture Collection (Beijing, China). In our experiments, BC cells specifically refer to MDA-MB-231 and MDA-MB-468 cells. All cells were maintained in Dulbecco’s modified Eagle medium (DMEM; Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Carlsbad, CA, USA), penicillin (100 U/mL), and streptomycin (100 mg/mL) (Gibco) at 37 °C with 5% CO₂. For hypoxia stimulation, BC cells were grown in a hypoxia chamber with 1% O₂ at particular times (0, 3, 6, 12, 24, and 48 h).
The small interfering RNA against circ_0001982 (si-circ_0001982) and its negative control (si-NC), the inhibitor of miR-1287-5p (anti-miR-1287-5p) and its negative control (anti-miR-NC), and the small interfering RNA against-MUC19 (si-MUC19) were bought from Ribobio (Guangzhou, China). Lentivirus based short hairpin RNA for circ_0001982 (sh-circ_0001982) and negative control (sh-NC) were constructed by GeneCopoeia (Rockville, MD, USA). These oligonucleotides were transfected into the BC cells using the Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer’s instructions.

Qiu Z., Wang L, & Liu H. (2021). Hsa_circ_0001982 promotes the progression of breast cancer through miR-1287-5p/MUC19 axis under hypoxia. World Journal of Surgical Oncology, 19, 161.

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Culturing Human Breast Cell Lines

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Human breast epithelial cell line MCF-10A (BNCC337734), human BC cell lines T47D (BNCC339607), MDA-MB-231 (BNCC339911), MCF-7 (BNCC100137) and BT-474 (BNCC101989) were bought from BeNa Culture Collection (Beijing, China) and cultured at 37 °C with 5% CO₂. Information of culture mediums:
MDA-MB-231, MCF-7, T47D cell lines: DMEM with 10% fetal bovine serum (FBS) and 100 U/mL penicillin/streptomycin;
MCF-10A, BT-474 cell lines: RPMI-1640 medium with 10% FBS.

Li X., Ren Y., Liu D., Yu X, & Chen K. (2022). Role of miR-100-5p and CDC25A in breast carcinoma cells. PeerJ, 10, e12263.

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Breast Cell Line Manipulation Protocol

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Human normal breast cell line MCF-10A and breast cancer cell lines HCC1937, MDA-MB-231 and MCF-7 were obtained from BeNa Culture Collection (Beijing, China). MCF-10A and HCC1937 cells were cultured in the completely culture medium containing 90% RPMI-1640 (Thermo Fisher Scientific, Waltham, MA, USA) and 10% FBS (Thermo Fisher Scientific); MDA-MB-231 cells were cultured in 90% L-15 cell culture medium (Thermo Fisher Scientific) and 10% FBS; MDA-MB-231 cells and MCF-7 cells were cultured in minimum essential medium (Thermo Fisher Scientific) supplemented with 10% FBS. All cells were maintained in a humidified incubator with 5% CO₂ in a constant temperature of 37°C.
To upregulate or downregulate the expression of PUF60, lentivirus containing PUF60 open reading frame (OE-PUF60) and shRNA targeting human PUF60 gene were purchased from OriGene (Beijing, China). And lentivirus (OE-PTEN) used to upregulate PTEN expression was designed and synthesized by GenePharma (Shanghai, China).

Sun D., Lei W., Hou X., Li H, & Ni W. (2019). PUF60 accelerates the progression of breast cancer through downregulation of PTEN expression. Cancer Management and Research, 11, 821-830.

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Breast Cancer Cell Line Cultivation

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Human normal breast epithelial cell line MCF-10A (BNCC337734) and human BC cell lines MDA-MB-231 (BNCC337893), MCF7 (BNCC337656), HCC1937 (BNCC338509), and MX-1 (BNCC100280) were offered by BeNa Culture Collection (Beijing, China). MCF-10A, HCC1937, and MX-1 were grown in 90% RPMI-1640 (HyClone, USA) + 10% fetal bovine serum (FBS; Thermo Scientific HyClone, Beijing, China). MDA-MB-231 and MCF7 were cultivated in DMEM (Thermo, USA) with 10% FBS.

Chu J., Li T., Li L, & Fan H. (2021). MicroRNA-139-5p Suppresses Cell Malignant Behaviors in Breast Cancer through Targeting MEX3A. Computational and Mathematical Methods in Medicine, 2021, 6591541.

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Exploring CDCA5 and PDS5A in Breast Cancer

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Human normal mammary MCF-10A and human breast cancer MCF-7, MDA-MB-231, SUM190PT and SK-BR-3 cell lines were purchased from BeNa Culture Collection and cultured in DMEM (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS (Gibco; Thermo Fisher Scientific, Inc.) under constant conditions at 37°C in a humidified atmosphere with 5% CO₂.
Short hairpin RNAs (shRNAs) against CDCA5 (shRNA-CDCA5-1, 5′-CCGGCCAAAGTACCATAGCCAGTTTCTCGAGAAACTGGCTATGGTACTTTGGTTTTTG-3′; and shRNA-CDCA5-2, 5′-CCGGGAGCAGTTTGATCTCCTGGTTCTCGAGAACCAGGAGATCAAACTGCTCTTTTTG-3′), scrambled shRNA [transfected with empty vector; shRNA-negative control (NC), 5′-TTCGGGTCATCCGATGGGCC-3′], adenovirus overexpressing PDS5A (pcDNA3.1-PDS5A) and control adenovirus (pcDNA3.1-NC) were constructed by Hanbio Biotechnology Co., Ltd. A total of 0.4 µg shRNA/adenovirus was transfected into 1×10⁵ cells using Lipofectamine^® 3000 (Invitrogen; Thermo Fisher Scientific, Inc.) and incubated for 10 min at room temperature, according to the manufacturer's instructions. At 48 h post-transfection, the transfected cells were used for subsequent experiments.

Wang Y., Yao J., Zhu Y., Zhao X., Lv J, & Sun F. (2022). Knockdown of CDCA5 suppresses malignant progression of breast cancer cells by regulating PDS5A. Molecular Medicine Reports, 25(6), 209.

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Celastrol Cytotoxicity in TNBC Cells

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Human TNBC cell lines MDA-MB-468 and MDA-MB-231 were purchased from BeNa Culture Collection (Beijing, China). The cells were grown in DMEM (Sigma, St. Louis, MO, USA) plus 10% fetal bovine serum (Sigma) and 1% penicillin/streptomycin (Sigma) at 37°C with 5% CO₂. The purity of celastrol (Sigma) was ≥98% and it was dissolved in DMSO (Sigma). Cells were incubated with various doses of celastrol (0.1, 0.5, 1, 2, and 5 μM) for 24 h based on methods described in previous reports [6 (link),15 (link)]. The high doses (2 and 5 μM) and low doses (0.1, 0.5, and 1 μM) of celastrol were differentiated according to the influence on cell viability. The control group was treated with an equal volume of DMSO. Subsequently, cells were collected for further experiments. Each sample was prepared in triplicate, and every experiment was repeated 3 times.

Yan F., Wu Z., Li Z, & Liu L. (2020). Celastrol Inhibits Migration and Invasion of Triple-Negative Breast Cancer Cells by Suppressing Interleukin-6 via Downregulating Nuclear Factor-κB (NF-κB). Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 26, e922814-1-e922814-9.

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Culturing Breast Cancer Cell Lines

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Normal breast epithelial cells (MCF-10A) and BC cells (MDA-MB-468, MCF7, and MDA-MB-231) were provided by the BeNa Culture Collection (Beijing, China) and cultured in high-glucose (HG) DMEM containing 10% fetal bovine serum (FBS). MDA-MB231 and MCF7 cells were cultivated in RPMI 1640 medium containing 10% FBS. Each sample was incubated at 37°C under 5% CO₂ conditions [37 (link)].

Chang J., Zhang Y., Ye X., Guo H., Lu K., Liu Q, & Guo Y. (2021). Long non-coding RNA (LncRNA) CASC9/microRNA(miR)-590–3p/sine oculis homeobox 1 (SIX1)/NF-κB axis promotes proliferation and migration in breast cancer. Bioengineered, 12(1), 8709-8723.

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TNBC Tumor Analysis and Cell Culture

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Twenty pairs of tissue from TNBC patients were analyzed. The criteria for TNBC included negativity for ER, PR and HER2, an original histological diagnosis of invasive breast carcinoma, and the efficiency of clinical pathological data. Specimens were frozen in liquid nitrogen (−80°C) for analysis. The study was approved by the Ethical Committee of Shandong Cancer Hospital affiliated to Shandong University (No.SDTHEC201001031) and each patient provided informed consent. MCF-7, MDA-MB-231 and MCF-10A cells were purchased from the Bena Culture Collection. Cells were cultured in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin and 1% non-essential amino acids and cultured at 5% CO₂ at 37°C. Medium was replaced every 2 d. When cells were 80–90% confluent, cells were passaged at a culture ratio of 1:1. Cells in the logarithmic growth phase were assessed for further experiments.

Song X., Liu Z, & Yu Z. (2020). EGFR Promotes the Development of Triple Negative Breast Cancer Through JAK/STAT3 Signaling. Cancer Management and Research, 12, 703-717.

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Establishing Paclitaxel-Resistant Breast Cancer Cell Lines

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Human breast epithelial cell line MCF-10A and human BC cell lines (MDA-MB-468, MDA-MB-453, MCF-7 and MDA-MB-231) were obtained from BeNa Culture Collection (Beijing, China). Human BC cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, Carlsbad, CA, USA) added with 10% fetal bovine serum (FBS; Gibco), 100 units/mL penicillin and 100 μg/mL streptomycin. MCF-10A cells were cultured in Roswell Park Memorial Institute-1640 medium (RPMI-1640, Gibco) supplemented with 10% FBS (Gibco) and 10% penicillin (100 U/mL)-streptomycin (100 μg/mL). All cells were grown in a 37°C, 5% CO₂ humidified incubator.
PTX was obtained from Sigma (St. Louis, MO, USA) and dissolved in dimethyl sulfoxide (DMSO; Sigma). The PTX-resistant BC cell lines, MCF-7/PTX and MDA-MB-231/PTX, were established by treating parental MCF-7 and MDA-MB-231 cells with increased concentration of PTX.22 (link)

Yang W., Gong P., Yang Y., Yang C., Yang B, & Ren L. (2020). Circ-ABCB10 Contributes to Paclitaxel Resistance in Breast Cancer Through Let-7a-5p/DUSP7 Axis. Cancer Management and Research, 12, 2327-2337.

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