All 3D-printed devices are designed using Autodesk Fusion 360 software (Autodesk Inc., USA). The micro-scaffold, pressurization module, and piston arrays were printed in-house by the Advanced-DLP technique (LED wavelength of 385 nm) using a D4Kpro 3D desktop printer (EnvisionTEC, Germany), with resolutions of 25 µm and 1 µm for the X-Y-axes and the Z-axis, respectively. The printer was calibrated following the manufacturer’s protocol. The layer thickness was set to 25 µm and the printing build style was provided by the manufacturer and was specific to the printing material. We selected HTM140V2 (EnvisionTEC, Germany) resin owing to its high-resolution printing capability, excellent strength (Tensile Strength of 56 MPa), exceptional heat resistance (Heat Deflection Temperature up to 140 °C), and smooth surface finishing of the printed items. For curing, the micro-scaffold, pressurization module, and piston arrays were rinsed with 100% methanol, dried up by N2 gas, then cured by ultraviolet (UV) at a wavelength of 405 nm for 4 min. The computer-aided design (CAD) files (.stl) for the micro-scaffold and pressurization module are provided as Source Data files.
Htm140v2
Manufactured by EnvisionTEC
Sourced in Germany, United States
The HTM140V2 is a high-temperature 3D printer developed by EnvisionTEC. It is designed for the production of high-performance parts and components. The HTM140V2 can print a variety of materials at temperatures up to 140°C.
Automatically generated - may contain errors
Lab products found in correlation
Fusion 360, by Autodesk (1 mentions)
Vida 3d printer, by EnvisionTEC (1 mentions)
Low molecular weight chitosan, by Merck Group (1 mentions)
Artificial saliva, by Merck Group (1 mentions)
Glucose oxidase from aspergillus niger, by Merck Group (1 mentions)
Potassium iodide ki, by Merck Group (1 mentions)
Peroxidase from horseradish hrp, by Merck Group (1 mentions)
Potassium chloride (kcl), by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
4 protocols using htm140v2
1
High-Resolution 3D-Printed Microfluidic Devices
2
Optimized 3D Printing of Sporulation Supports
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The sporulation supports were designed using computer‐aided design (CAD) with the software Siemens NX 1859. Manufacturing was performed with a Vida 3D printer (EnvisionTEC GmbH) using the principle of inverse digital light processing (inverse DLP) at a temperature of 23°C. The pixel width of the light projector with a power of 330 W was 73 × 73 μm at a resolution of 1920 × 1080. The printing process was performed with a layer height of 50 μm. For this purpose, the components created in the CAD software were divided into individual layers using Perfactory Rapid Prototyping 3.2.3377.1712 software. The resins used were HTM 140 V.2 (EnvisionTEC GmbH) and E‐Shell 600 clear (DeltaMed GmbH). E‐Shell 600 clear is certified according to the United States Pharmacopeia (USP) as Class VI and thus is biocompatible according to ISO 10993, making it suitable for pharmaceutical applications.
3
Fabrication of Microfluidic Device
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The microfluidic device was printed with EnvisionTEC Micro Hi-Res Plus 3D printer using the resin HTM 140 V2 (EnvisionTEC), with the following printing parameters (set automatically based on the resin used): burn-in range thickness 400 μm, base plate of 300 μm, and exposure time 3000 ms. The printed device was washed using ethanol and air dried using an air gun. A thin layer of PDMS (polydimethylsiloxane) at a ratio of 1:10 (curing agent: elastomer) was put on the cone of the chip with the help of a syringe needle and baked at 70 °C for 30 minutes and subsequently baked using a UV chamber for 10 minutes. To ensure hydrophobicity and reduce the diameter of the device tip, Bohlender PTFE tubing was used. The tubing was cut under a stereo binocular (Leica) using a scalpel to obtain a size of around 200 - 300 μm in length and glued on the tip of the microfluidic device with epoxyglue EA M-31CL (Loctite) and left to solidify for 1h at RT. To make the inlets, three 19-gauge stainless steel needles were cut into segments 1:5 cm long and polished using a Dremel 8000 WorkStation to avoid sharp edges. A small droplet of glue EA M-31CL was spread at the edges of the needles and they were inserted into the inlets of the devices, after which the glue was left to solidify for 24 hours at RT.
4
Glucose Oxidase-based Biosensing Protocol
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Phosphate buffered saline (PBS) (Sigma Aldrich, USA), glucose oxidase from Aspergillus niger (211 U/mg) (Sigma Aldrich, USA), peroxidase from horseradish (HRP, 325 U/mg) (Sigma Aldrich, USA), potassium chloride (KCl) (Sigma Aldrich, USA), potassium iodide (KI) (Sigma Aldrich, USA), chitosan-low molecular weight (Sigma Aldrich, USA), acetic acid-2% (v/v) in H2O (Sigma Aldrich, USA), D(+)-glucose (C6H12O6) -≥99.5% (GC) (Sigma Aldrich, USA), paper-towel (Eagle photocell towel, Eagle Professional, Turkey), Self-inking stamp (Kas ¸em, Turkey), methacrylic/acrylic resin (HTM140 V2, EnvisionTEC, USA), thermoplastic filament (Z-ABS, Zortrax, USA), Whatman qualitative filter paper-grade 1 (Sigma Aldrich, USA), artificial saliva (NeutraSal, Germany).
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