Anti nfatc1 antibody

RANKL was purchased from Peprotech (London, UK). Alpha-minimum essential media (α-MEM), fetal bovine serum (FBS), penicillin/streptomycin (P/S) and Dulbecco’s phosphate buffered saline (DPBS) were obtained from Gibco (Gaithersburg, NY, USA). TRAP assay kit was obtained from Sigma Aldrich (Saint Louis, MO, USA). Osteo assay surface multiple well plates were obtained from Corning, Inc. (New York, NY, USA). Anti-c-Fos antibody, anti-TRAF6 antibody and anti-β-actin antibody were obtained from Santa Cruz (CA, USA). Anti-NFATc1 antibody was purchased from BD Pharmingen (San Diego, CA, USA). Anti-MMP-9 antibody and anti-CTK antibody were purchased from Abcam (Cambridge, MA, USA). Anti-total-ERK antibody, anti-phospho ERK antibody, Anti-total-JNK antibody, anti-phospho JNK antibody, Anti-total-p38 antibody and anti-phospho p38 antibody were purchased from Cell signaling (Beverly, MA, USA). Anti-NFATc1 antibody was purchased from BD Pharmingen (San Diego, CA, USA).PCR primers were obtained from Genotech (Daejeon, Korea). All of the chemicals used in the experiments were of analytical grade or complied with the level required for cell culture.

Antibodies against phospho-p38, phospho-ERK, phospho-JNK, phospho-Akt, phospho-IκBα, and phospho-p65 were purchased from Cell Signaling Technology (Danvers, MA, USA). Monoclonal anti-β-actin antibody was obtained from Sigma-Aldrich (St. Louis, MO, USA), and anti-Nfatc1 antibody was purchased from BD Pharmingen (San Diego, CA, USA). Recombinant M-CSF and RANKL were purchased from R & D Systems (Minneapolis, MN, USA). DPHC was isolated from Ishige okamurae, and the method of isolation as well as the purity and structure of DPHC were confirmed by spectroscopy [9 (link)].