Log In Sign up
The largest database of trusted experimental protocols

Fluorescein isothiocyanate fitc labeled albumin

Manufactured by Merck Group
Visit Supplier
Sourced in United States

Fluorescein isothiocyanate (FITC)-labeled albumin is a fluorescent dye-labeled protein compound. It is used as a tool in various research and analytical applications.

Automatically generated - may contain errors

Lab products found in correlation

Trypsin ethylenediaminetetraacetic acid, by Thermo Fisher Scientific (1 mentions) Pkh67, by Thermo Fisher Scientific (1 mentions) Lipopolysaccharide lps, by Merck Group (1 mentions) Celltracker red cmtpx, by Thermo Fisher Scientific (1 mentions) Collagen type 1, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions) Chloroform, by Merck Group (1 mentions) Interleukin 4 (il 4), by R&D Systems (1 mentions) Granulocyte macrophage colony stimulating factor, by R&D Systems (1 mentions) Eclipse fn1, by Nikon (1 mentions) 5 mm coverslip, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

FITC (1014 citations) FITC-albumin (64 citations) FITC-labeled albumin (16 citations) Fluorescein isothiocyanate (FITC)-albumin (5 citations) Bovine fluorescein isothiocyanate (FITC)–labeled albumin (5 citations) Albumins ( (2 citations) Fluorescein isothiocyanate (FITC)-labeled bovine serum albumin (BSA) (2 citations) Fluorescein isothiocyanate-labeled bovine serum albumin (FITC-BSA) (2 citations)

3 protocols using fluorescein isothiocyanate fitc labeled albumin

1

Immune Cell Activation in Biomimetic Platform

Check if the same lab product or an alternative is used in the 5 most similar protocols
PCL (Mn =700,000–900,000), chloroform, fluorescein isothiocyanate (FITC)-labeled albumin, and lipopolysaccharide (LPS) were purchased from Sigma-Aldrich (St Louis, MO, USA). Dulbecco’s Modified Eagle’s Medium, fetal bovine serum (FBS), and 0.05% trypsin–ethylenediaminetetraacetic acid were purchased from Gibco (Rockville, MD, USA). Granulocyte macrophage-colony stimulating factor, interleukin-4, carboxyfluorescein succinimidyl ester, FITC–phalloidin, and antibodies against CD11, CD86, major histocompatibility complex (MHC) Class II, calreticulin, phosphorylated focal adhesion kinase (p-FAK), and reticular fiber were obtained from R&D Systems Inc. (Minneapolis, MN, USA). 4′,6-Diamidino-2-phenylindole (DAPI) and CellTracker Red CMTPX were purchased from Invitrogen (Carlsbad, CA, USA). Collagen Type I was supplied by Merck Millipore (Darmstadt, Germany). Fluorescent membrane dyes, PKH26 and PKH67, were purchased from Molecular Probes, Inc. (Eugene, OR, USA). Polydimethylsiloxane (PDMS) was purchased from Dow-Corning Korea, Inc. (Seoul, South Korea).
Kim T.E., Kim C.G., Kim J.S., Jin S., Yoon S., Bae H.R., Kim J.H., Jeong Y.H, & Kwak J.Y. (2016). Three-dimensional culture and interaction of cancer cells and dendritic cells in an electrospun nano-submicron hybrid fibrous scaffold. International Journal of Nanomedicine, 11, 823-835.
+ Open protocol
+ Expand
2

Intravital Microscopy of Pial Microcirculation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Thirty-two hours after CCI, a second 2.5-mm craniotomy was created in front of the first and covered with a 5-mm coverslip (Fisher Scientific, Waltham, MA). Animals were then transferred to an intravital microscope (ECLIPSE FN1; Nikon Instruments, Melville, NY) and received a 50-μL IV bolus of .3% rhodamine 6G to fluorescently label circulating leukocytes. Footage of the pial microcirculation was recorded under a 590-nm epi-illumination emission filter using a digital camera (QuantEM; Photometrics, Tucson, AR) for subsequent analysis by a blinded observer (Fig. 2A). Nonbranching postcapillary venules (25 to 50-μm diameter) were selected for 1-minute recordings in each animal. Once this recording was completed, 100 mg/kg fluorescein isothiocyanate (FITC)-labeled albumin (Sigma Aldrich) was administered and the same pial regions were visualized under a 488-nm fluorescent filter for determination of albumin leakage immediately outside the selected venules.
Kumasaka K., Marks J.A., Eisenstadt R., Murcy M.A., Samadi D., Li S., Johnson V., Browne K.D., Smith D.H., Schwab C.W, & Pascual J.L. (2014). In vivo leukocyte-mediated brain microcirculatory inflammation: a comparison of osmotherapies and progesterone in severe traumatic brain injury. American journal of surgery, 208(6), 961-968.
+ Open protocol
+ Expand
3

In Situ Rat Lung Fluid Clearance

Check if the same lab product or an alternative is used in the 5 most similar protocols
AFC was determined using an in situ rat-lung model, as described previously (37 (link)). Briefly, at the end of reperfusion, rat lungs were inflated with 100% oxygen at 7 cm H2O continuous positive airway pressure. Then, instillate containing fluorescein isothiocyanate (FITC)-labeled albumin (Sigma-Aldrich, St. Louis, MO) was delivered to the lungs over 1 min at 12.5 mL/kg of body weight of. An alveolar fluid sample (100 μL) was collected 1 min after instillation and 15 min later. The samples were centrifuged at 3,000 × g for 10 min, and the fluorescence activity in the supernatant was measured in duplicate. AFC was computed from the increase in alveolar fluid albumin concentration using the following equation:
AFC=(Cf-Ci)/Cf×100,
where Ci and Cf represent the initial and final concentrations of FITC-albumin in the aspirate at 1 and 15 min, respectively, as assessed by the fluorescence activity measurements.
Shen C.H., Lin J.Y., Chang Y.L., Wu S.Y., Peng C.K., Wu C.P, & Huang K.L. (2018). Inhibition of NKCC1 Modulates Alveolar Fluid Clearance and Inflammation in Ischemia-Reperfusion Lung Injury via TRAF6-Mediated Pathways. Frontiers in Immunology, 9, 2049.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!