H&E staining was carried out according to the following method. After deparaffinization, sections were stained with Mayer’s hematoxylin solution (Wako, Tokyo, Japan) for 10 min, and then stained with eosin alcohol solution (Wako) for 4 min. Finally, the sections were dehydrated and coverslipped. Terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling (TUNEL) staining was carried out using the DeadEnd Fluorometric TUNEL System (Promega Madison, WI), according to the manufacturer’s instructions.
Eosin alcohol solution
Manufactured by Fujifilm
Sourced in Japan
Eosin alcohol solution is a lab equipment product manufactured by Fujifilm. It is a ready-to-use solution that contains the dye eosin dissolved in alcohol. The primary function of this product is to provide a staining solution for microscopic analysis and examination of biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Mayer s hematoxylin solution, by Fujifilm (4 mentions)
Deadend fluorometric tunel system, by Promega (1 mentions)
Formalin neutral buffer solution, by Fujifilm (1 mentions)
Bx53 microscope, by Olympus (1 mentions)
Fluoro jade c, by Merck Group (1 mentions)
Permount, by Thermo Fisher Scientific (1 mentions)
Bz 8000 microscope, by Keyence (1 mentions)
Mayer hematoxylin solution, by Fujifilm (1 mentions)
Mayer s hematoxylin, by Muto Pure Chemicals (1 mentions)
6 protocols using eosin alcohol solution
1
Histological Analysis of Tissue Samples
2
Histological Analysis of Organ Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice were euthanized and organs were removed in PBS containing 2% FBS. Thereafter, organs were fixed with 15% formalin neutral buffer solution (Cat#: 067–02397; FUJIFILM Wako Pure Chemical Corporation), and 4‐μm thick paraffin‐embedded sections were prepared. Next, H&E staining was carried out using Mayer's hematoxylin solution (Cat #: 131–09665; FUJIFILM Wako Pure Chemical Corporation), eosin alcohol solution, and acid extract (Cat #: 050–06041; FUJIFILM Wako Pure Chemical Corporation). Finally, images were captured using a BX53 microscope (Olympus Corporation).
3
Histochemical Staining Protocols for Tissue Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
H&E staining was carried out according to the following method. After deparaffinization, sections were stained with Mayer's hematoxylin solution (Wako, Tokyo, Japan) for 10 min, and then stained with eosin alcohol solution (Wako) for 4 min. Finally, the sections were dehydrated and coverslipped using NEW M.X (Matsunami-glass). FluoroJade C (#3,319,822; Merck Millipore, Burlington, MA, USA) staining was carried out according to the manufacturer’s instructions. Briefly, frozen sections were treated with basic ethanol (80% ethanol/1% NaOH) for 5 min, and washed in 70% EtOH and ultrapure water for 2 min, respectively. The sections were then incubated in 0.06% potassium permanganate. After washing in ultrapure water for 2 min, the sections were treated with the staining solution (Fluoro-Jade C and 2 µg/mL Hoechst diluted in 0.1% acetic acid). After washing in ultrapure water, the sections were heated at 60 °C, air dried, and incubated in xylene for 5 min. Finally, the sections were coverslipped using NEW M.X (Matsunami-glass). Floro-Styryl-Benzene (FSB) (Dojindo, Tokyo, Japan) staining was carried out according to the manufacturer's instructions. The sections were then incubated with FSB solution, and then soaked in lithium carbonate solution. After washing with 50% EtOH, the sections were coverslipped.
4
Hematoxylin and Eosin Staining Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Hematoxylin and Eosin (HE) staining was conducted following a previously described protocol (Ueda et al., 2016 (link)). Briefly, brain sections were washed with PBS, immersed in Mayer’s Hematoxylin solution (WAKO, Osaka, Japan) for 5 min at 25 °C and then washed with tap water for 20 min. After a brief treatment with 95% ethanol, sections were immersed in eosin–alcohol solution (WAKO) for 4 min at 25 °C. Sections were dehydrated through a series of ethanol solutions, xylene, and over-slipped with Permount (Fisher Scientific, Waltham, MA, USA). The analysis of the HE-stained brain sections was performed using a BZ-8000 microscope with BZ Image Measurement Software (KEYENCE, Osaka, Japan). The brightness and contrast of all images were adjusted in Adobe Photoshop (Adobe Systems Inc., San Jose, CA) to the same conditions.
5
Histological Analysis of Tissue Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tissues from the skin, lung, liver, and SI were fixed with 4% paraformaldehyde (PFA) in PBS and embedded in paraffin (26 (link)). Tissue sections were stained with hematoxylin and eosin (H&E). In brief, paraffin-embedded tissues were cut into 5-μm-thick sections and placed onto silane-coated slide glasses. The sections were deparaffinized with toluene and rehydrated through graded ethanol series. Tissues were stained with Mayer Hematoxylin Solution (FUJIFILM Wako Pure Chemical, 131–09665) and Eosin Alcohol Solution (FUJIFILM Wako Pure Chemical, 050–06041), then dehydrated with graded ethanol series and cleared with xylene (FUJIFILM Wako Pure Chemical, Wako 242–00087; ref. 26 (link)). The stained slides were examined with a bright-field microscopy (BX53; Olympus).
6
Hematoxylin-Eosin Staining of Frozen Sections
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Hematoxilyn-Eosin (HE) staining: frozen sections of 10 µm were stained in Mayer's Hematoxylin (Muto Pure Chemicals CO., LTD, 3000-2) for 10 seconds, and washed in running tap water. Following a replacement of ethanol solution, the sections were stained in Eosin Alcohol Solution (Wako, 050-06041) for 5 minutes. After dehydration in an ethanol series, the sections were cleared in xylene and mounted.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!