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Lissamine rhodamine b 1 2 dihexadecanoyl sn glycero 3 phosphoethanolamine triethylammonium salt rh pe

Manufactured by Thermo Fisher Scientific
Sourced in United States

Lissamine™ rhodamine B 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine triethylammonium salt (Rh-PE) is a fluorescent lipid probe used for labeling and tracking lipid-containing structures. It is a derivative of the rhodamine B dye conjugated to a phospholipid.

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3 protocols using lissamine rhodamine b 1 2 dihexadecanoyl sn glycero 3 phosphoethanolamine triethylammonium salt rh pe

1

Lipid Bilayer Preparation and Characterization

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Non-hydrogenated egg phosphatidylcholine (PC) (Coatsome NC-50; PC purity≥95%) was purchased from NOF Corporation (Tokyo, Japan). Cholesterol (Chol) was purchased from Sigma-Aldrich, St Louis, MO, USA. Tween 20 was purchased from Ajax Finechem (Auckland, New Zealand). Sodium fluorescein (NaFl), d-limonene, 1,8-cineole, and geraniol were purchased from Sigma-Aldrich. 5-DSA and 16-DSA were purchased from Sigma-Aldrich. Lissamine™ rhodamine B 1, 2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine triethylammonium salt (Rh-PE) was purchased from Invitrogen, CA, USA. All other reagents were of analytical grade and were commercially available.
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2

Lipid-Based Nanoparticle Preparation

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Egg phosphatidylcholine (PC) and Na-salt N-(carbonyl-methoxypolyethylen glycol-2000)-1,2-distearoyl-sn-glycero-3-phosphoethanolamine (PEG2000-DSPE) were purchased from Lipoid GmbH, Ludwigshafen, Germany. Cholesterol (Chol) was purchased from Carlo Erba Reagent, Ronado, Italy. Tween 20 was purchased from Ajax Finechem, Auckland, New Zealand. NaFI and d-limonene were purchased from Sigma-Aldrich, St Louis, MO, USA. Lissamine™ rhodamine B 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine triethylammonium salt (Rh-PE) was purchased from Invitrogen, Carlsbad, CA, USA.
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3

Liposome Formulation Components Characterization

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Materials Egg phosphatidylcholine (PC) and Na-salt PEG2000-DSPE were purchased from Lipoid GmbH, Ludwigshafen, Germany. Cholesterol (Chol) was purchased from Carlo Erba Reagent, Ronado, Italy. Tween 20 ® was purchased from Ajax Finechem, Auckland, New Zealand. Tween 80 ® was purchased from the NOF Corporation (Osaka, Japan). Cetyl palmitate (CP) was purchased from SABO SpA (Levate, Italy). Sodium fluorescein (NaFI), d-limonene, and Span 20 were purchased from Sigma-Aldrich, MO, U.S.A. Lissamine™ rhodamine B 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine triethylammonium salt (Rh-PE) was purchased from Invitrogen, CA, U.S.A.
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