Rabbit polyclonal anti caspase 9

Protein levels of dCK, dGK, caspase-3, caspase-9, Bcl2, Bcl-xL, Bax, Bad, Bid, Bim, AKT, and p-AKT were determined by using standard western blotting techniques [13 (link)]. Mouse monoclonal anti-dCK was developed in the Department of Pediatrics of Mie University School of Medicine [13 (link)]. Rabbit polyclonal anti-dGK antibody (Abgent, San Diego, CA, USA), rabbit polyclonal anti-caspase-3 (Cell Signaling Technology, Beverly, MA, USA), rabbit polyclonal anti-caspase-9 (Cell Signaling Technology), rabbit polyclonal anti-Bcl-2 (Cell Signaling Technology), rabbit polyclonal anti-Bcl-xL (Cell Signaling Technology), rabbit polyclonal anti-Bax (Cell Signaling Technology), rabbit polyclonal anti-Bad (Cell Signaling Technology), rabbit polyclonal anti-Bid (Cell Signaling Technology), rabbit polyclonal anti-Bim (Cell Signaling Technology), rabbit polyclonal anti-AKT (Cell Signaling Technology), rabbit polyclonal anti-P-AKT (Santa Cruz Biotechnology, Inc. Dallas, TX, USA), and anti-actin antibodies (Sigma-Aldrich) were used as primary antibodies [13 (link)].

Western blotting analysis was performed as the previous study.^{19 (link)} Antibodies used were as following: rabbit polyclonal anti-PARP, rabbit polyclonal anti-caspase-3, rabbit polyclonal anti-cleaved caspase-3, rabbit polyclonal anti-caspase-9, rabbit polyclonal anti-cleaved caspase-9, rabbit polyclonal anti-cyclin B1, mouse polyclonal anti-cdc2 and rabbit polyclonal anti-p-cdc2 (Tyr15) were purchased from Cell Signaling Technology (Beverly, MA, USA). Mouse polyclonal anti-MPM2 and rabbit polyclonal anti-BICD2 were commercially available from Millipore Corporation (Bedford, MA, USA), and rabbit polyclonal anti-FOXM1 antibody was purchased from ABclonal Technology (Wuhan, China). Mouse polyclonal anti-β-actin was purchased from Sunshine Biotechnology Ltd. Goat polyclonal anti-rabbit IgG conjugated to HRP and goat polyclonal anti-mouse IgG conjugated to HRP (Cell Signaling Technology) were used as secondary antibodies, and enhanced chemiluminescence reagents (Millipore) was used for detection and exposed by Gel 2000 image analyzer (Bio-Rad, Richmond, CA, USA).