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Anti tsg101 rabbit polyclonal antibody

Manufactured by Abcam
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Sourced in United Kingdom

Anti-TSG101 rabbit polyclonal antibody is a laboratory reagent used to detect the presence and quantify the levels of the TSG101 protein in biological samples. TSG101 is a key component of the ESCRT (Endosomal Sorting Complexes Required for Transport) machinery and plays a role in various cellular processes.

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Lab products found in correlation

Ripa buffer, by Thermo Fisher Scientific (6 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (6 mentions) Protease and phosphatase inhibitor cocktail, by Roche (3 mentions) Nupage 4 12 bis tris gel, by Thermo Fisher Scientific (2 mentions) Mouse monoclonal anti gm130 antibody, by BD (2 mentions) Anti rab5 mouse monoclonal antibody, by Synaptic Systems (2 mentions) Chemiluminescence reagent, by Sartorius (1 mentions) Anti hrp conjugate, by Thermo Fisher Scientific (1 mentions) Nupage 4 12 bis tris protein gel, by Thermo Fisher Scientific (1 mentions) Chemidoc system, by Bio-Rad (1 mentions)

3 protocols using anti tsg101 rabbit polyclonal antibody

1

Characterization of Extracellular Vesicles from iPS Cells

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iPS cells, iCMs, and EVs isolated from these cells were washed with ice cold PBS followed by lysis in RIPA buffer (ThermoFisher Scientific, Waltham, MA) supplemented with protease and phosphatase inhibitor cocktails (Roche, Basel, Switzerland), followed by centrifugation at 16,000g for 15 minutes at 4°C. Protein concentration of both the cell lysate and EV lysate were determined by Pierce BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA). For Western blotting, lysates were fractionated via SDS-PAGE on NuPage 4–12% BisTris gels (ThermoFisher Scientific, Waltham, MA) and transferred onto nitrocellulose membranes for immunoblotting.
Antibodies were used at the following concentrations: anti-TSG101 rabbit polyclonal antibody (1:1000, abcam, Cambridge, UK), anti-GM130 mouse monoclonal antibody (1:500, BD Transduction Laboratories, Franklin Lakes, NJ), anti-Rab5 mouse monoclonal antibody (1:1000, Synaptic Systems, Goettingen, Germany).
Liu B., Lee B.W., Nakanishi K., Villasante A., Williamson R., Metz J., Kim J., Kanai M., Bi L., Brown K., Di Paolo G., Homma S., Sims P.A., Topkara V.K, & Vunjak-Novakovic G. (2018). Cardiac recovery via extended cell-free delivery of extracellular vesicles secreted by cardiomyocytes derived from induced pluripotent stem cells. Nature biomedical engineering, 2(5), 293-303.
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2

EV Protein Characterization by Western Blot

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THP-1-derived MΦs and isolated EVs were lysed in RIPA Buffer (Thermo Fisher Scientific) supplemented with protease and phosphatase inhibitor cocktails (Roche), followed by centrifugation at 14,000 ×g for 15 min at 4°C. Protein quantities of the lysates were quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). For Western blotting, fractionation of lysates was carried in sodium dodecyl sulfate polyacrylamide gel electrophoresis on NuPAGE 4%–12% Bis-Tris Protein Gels (Thermo Fisher Scientific). Then, fractionated samples were transferred onto nitrocellulose membranes for immunoblotting. The membranes were blocked in Tris-buffered saline containing 5% powdered bovine serum albumin (BSA) for 1 h at room temperature, and then incubated overnight at 4°C with anti-TSG101 rabbit polyclonal antibody (Abcam) and anti- Calnexin rabbit polyclonal antibody (Abcam), which were diluted 1:1000. After incubation with an appropriate anti- HRP conjugate (Thermo Fisher Scientific), bands were visualized with a chemiluminescence reagent (Biological Industries) and detected using the ChemiDoc system (Bio Rad, CA).
Bar A., Kryukov O., Etzion S, & Cohen S. (2023). Engineered extracellular vesicle-mediated delivery of miR-199a-3p increases the viability of 3D-printed cardiac patches. International Journal of Bioprinting, 9(2), 670.
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3

Characterization of Extracellular Vesicles from iPS Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
iPS cells, iCMs, and EVs isolated from these cells were washed with ice cold PBS followed by lysis in RIPA buffer (ThermoFisher Scientific, Waltham, MA) supplemented with protease and phosphatase inhibitor cocktails (Roche, Basel, Switzerland), followed by centrifugation at 16,000g for 15 minutes at 4°C. Protein concentration of both the cell lysate and EV lysate were determined by Pierce BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA). For Western blotting, lysates were fractionated via SDS-PAGE on NuPage 4–12% BisTris gels (ThermoFisher Scientific, Waltham, MA) and transferred onto nitrocellulose membranes for immunoblotting.
Antibodies were used at the following concentrations: anti-TSG101 rabbit polyclonal antibody (1:1000, abcam, Cambridge, UK), anti-GM130 mouse monoclonal antibody (1:500, BD Transduction Laboratories, Franklin Lakes, NJ), anti-Rab5 mouse monoclonal antibody (1:1000, Synaptic Systems, Goettingen, Germany).
Liu B., Lee B.W., Nakanishi K., Villasante A., Williamson R., Metz J., Kim J., Kanai M., Bi L., Brown K., Di Paolo G., Homma S., Sims P.A., Topkara V.K, & Vunjak-Novakovic G. (2018). Cardiac recovery via extended cell-free delivery of extracellular vesicles secreted by cardiomyocytes derived from induced pluripotent stem cells. Nature biomedical engineering, 2(5), 293-303.
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