SH-SY5Y cells (KCLB, Seoul, Korea) were cultured and maintained in Dubelcco's Modified Eagle Media (DMEM) Ham's F-12 (DMEM/F12) (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 15% heat inactivated fetal bovine serum (Sigma-Aldrich) and 1% penicillin/streptomycin (Sigma-Aldrich). The cells were incubated at 37°C in a humidified incubator containing 5% CO2. For differentiation of SH-SY5Y cells, cells were treated with 10 μM all-trans retinoic acid (RA) (Sigma-Aldrich) with 5% fetal bovine serum in DMEM/F12 media. The retinoic acid was dissolved (10 mM) in dimethyl sulfoxide, and freshly diluted further in culture medium.
Dmem ham s f 12 dmem f12
Manufactured by Merck Group
Sourced in United States
DMEM/F12 is a cell culture medium commonly used for the in vitro cultivation of a variety of cell types, including human and animal cells. It is a combination of Dulbecco's Modified Eagle's Medium (DMEM) and Ham's F-12 Nutrient Mixture. DMEM/F12 provides a balanced salt solution, amino acids, vitamins, and other nutrients required for cell growth and maintenance.
Automatically generated - may contain errors
Lab products found in correlation
Sh sy5y cells, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
Dmem f12, by Merck Group (1 mentions)
Basic fgf, by Fujifilm (1 mentions)
2 mercaptoethanol 2 me, by Thermo Fisher Scientific (1 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions)
Knockout serum replacement (ksr), by Thermo Fisher Scientific (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
2 protocols using dmem ham s f 12 dmem f12
1
SH-SY5Y Cell Differentiation with Retinoic Acid
2
Generation of VCPL198W iPSCs from FTLD-VCP Patients
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Patient dermal fibroblasts (GM20926) carrying the VCPL198W mutation, isolated from a FTLD-VCP patient, were obtained from Coriell Institute. iPS cells (iPSCs) with the VCP mutation were generated from the patient fibroblasts using episomal vectors for OCT3/4, Sox2, Klf4, L-Myc, Lin28, and p53-shRNA, as previously reported (Okita et al, 2013 (link)). Generated iPSCs were cultured on an SNL feeder layer with human iPSC medium (primate embryonic stem cell medium; ReproCELL) supplemented with 4 ng/ml basic FGF (Wako Chemicals) and penicillin/streptomycin.
For in vitro three-germ layer assays, iPSCs were dissociated and transferred to suspension plates with DMEM/Ham’s F12 (DMEM/F12; Sigma-Aldrich) containing 20% knockout serum replacement (KSR; Life Technologies), 2 mM L-glutamine, 0.1 mM nonessential amino acids (NEAA; Invitrogen), 0.1 mM 2-mercaptoethanol (2-ME; Life Technologies), and 0.5% penicillin and streptomycin, and cultured to generate embryoid bodies. On day 8, embryoid bodies were moved onto gelatin-coated plates and differentiated for an additional 8 d, followed by immunostaining analysis. We confirmed the pluripotency of the resultant VCPL198W-iPSCs.
For in vitro three-germ layer assays, iPSCs were dissociated and transferred to suspension plates with DMEM/Ham’s F12 (DMEM/F12; Sigma-Aldrich) containing 20% knockout serum replacement (KSR; Life Technologies), 2 mM L-glutamine, 0.1 mM nonessential amino acids (NEAA; Invitrogen), 0.1 mM 2-mercaptoethanol (2-ME; Life Technologies), and 0.5% penicillin and streptomycin, and cultured to generate embryoid bodies. On day 8, embryoid bodies were moved onto gelatin-coated plates and differentiated for an additional 8 d, followed by immunostaining analysis. We confirmed the pluripotency of the resultant VCPL198W-iPSCs.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!