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Rabbit anti rab14

Manufactured by Merck Group

Rabbit anti-Rab14 is an antibody product developed by Merck Group for use in laboratory research. It is a purified polyclonal antibody raised in rabbits against the Rab14 protein. Rab14 is a member of the Rab family of small GTPases that function in intracellular membrane trafficking. The antibody can be used to detect and study the Rab14 protein in various experimental systems.

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2 protocols using rabbit anti rab14

1

Epithelial Cell Tight Junction Analysis

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Cell culture reagents were obtained from Mediatech (Manassas, VA). The following antibodies were used: rabbit anti-Rab14 (AVIVA, San Diego, CA), rabbit anti-Rab14 (Sigma-Aldrich, St. Louis, MO), rat anti–E-cadherin (DECMA; Sigma-Aldrich), mouse anti-gp135 (gift from K. Matlin, University of Cincinnati, Cincinnati, OH), and rabbit anti–claudin-1, rabbit and mouse anti–claudin-2, mouse anti–claudin-4, and rabbit and mouse anti-occludin (all from Invitrogen, Grand Island, NY). Alexa Fluor secondary antibodies were also from Invitrogen. All other chemicals and reagents were from Sigma-Aldrich unless otherwise specified.
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2

Protein Expression and Localization

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Common chemicals and reagents were purchased from Sigma-Aldrich (St. Louis, MO). ZIP was from AnaSpec (Fremont, CA). The following antibodies were used: rabbit or mouse anti–claudin-1, rabbit or mouse anti–claudin-2, mouse anti–ZO-1, rabbit or mouse anti-occludin from Life Technologies (Grand Island, NY). Rabbit anti-Rab14, mouse anti–β-actin, mouse anti–E-cadherin and rabbit anti–E-cadherin were purchased from Sigma-Aldrich. Rabbit anti-aPKC was from Santa Cruz Biotechnology (Dallas, TX), and mouse anti-PKCι was from BD Biosciences (San Jose, CA). Alexa Fluor secondary antibodies were from Life Technologies.
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