Spss softwares

Comparison between two groups was assessed with the two-tailed unpaired Student's t test, whereas multiple comparisons were assessed with the one-way ANOVA or two-way ANOVA followed by Bonferroni's post-hoc test. General linear mixed model was used to analyse rotarod data. Kaplan-Meier method was used to analyze incubation times and comparison between groups was made with the log-rank test. GraphPad and IBM SPSS Softwares were used for analysis and p-values <0.05 were considered statistically significant. For statistical analysis, logarithm transformation was performed of numerical data in fig. 1K, and square root transformation of numerical data in fig. 3B. The details of each analysis (statistical test, p-values, n) are indicated in the figure legends.

Mixed design analysis of variance (ANOVA) with repeated measures, two-way ANOVA with repeated measures, one-way ANOVA with or without repeated measures were employed to analyze the data in Sigmaplot (Systat Software Inc., CA, USA) or SPSS softwares (IBM, NY, USA). A p-value of less than 0.05 was considered statistically significant. The data on proportion of M1 cells expressing Brn3b were transformed for statistical comparison using arcsine transformation. A two-tailed (paired or unpaired) t-test was used for some comparisons.

Statistical analysis of mean values was performed using Sigmaplot v12.0 (Systat Software, San Jose, CA, USA) and GraphPad Prism (San Diego, CA, USA) and SPSS softwares (IBM, Armonk, NY, USA). Statistical significance between control and experimental groups were calculated using Student's t-test for independent samples, one-way ANOVA and Bonferroni's multiple comparison post hoc test were performed where applicable, establishing the level of significance at P<0.05. Values are expressed as mean±S.E.M.