Goat anti perilipin

Adipose tissues and fat grafts were fixed in 4% paraformaldehyde, processed, and embedded in paraffin. The samples were then sectioned at a thickness of 5 µm and stained with hematoxylin and eosin (H & E). For immunohistological analysis, tissue sections were incubated with goat anti-perilipin (Abcam, Boston, MA, USA) and isolectin B4 (Vector Laboratories, Newwork, CA, USA), followed by incubation with a horseradish peroxidase-conjugated secondary antibody (Vector Laboratories, Burlingame, CA, USA) or Alexa Fluor 568-conjuaged secondary antibodies (Invitrogen, Waltham, MA, USA). For Oil Red O staining, cells were fixed with 4% paraformaldehyde, rinsed with deionized water, and incubated with Oil Red O staining solution (5 ng/mL) for 5 min. Images were captured using a Leica DM1000 light microscope (Leica Microsystems, Wetzlar, Germany) or a Leica DMi8 fluorescence microscope (Leica Microsystems, Wetzlar, Germany).