Prism 4 software version 4

EVs were analyzed by nanoparticle tracking, using the NanoSight NS300 system (Malvern, Great Malvern, UK). Samples were administered and recorded under controlled flow, using the NanoSight syringe pump and script control system, and for each sample, 5 videos of 60 s duration were recorded, with a 10-s delay between recordings, generating 5 replicate histograms that were averaged. Therefore, the typical number of completed tracks per sample was ~ 1,200. The area under the curve was calculated using Prism-4 software version 4.03 (Graph Pad, San Diego, CA), to give average particle counts from these replicates.

Vesicles were analyzed by nanoparticle tracking, using the NanoSight NS300 system (Malvern, Great Malvern, UK). Samples were administered and recorded under controlled flow, using the NanoSight syringe pump and script control system, and for each sample, 5 videos of 60 seconds duration were recorded, with a 10-second delay between recordings, generating 5 replicate histograms that were averaged. Therefore, the typical number of completed tracks per sample was approximately 1,200. The area under the curve was calculated using Prism-4 software version 4.03 (Graph Pad, San Diego, CA), to give average particle counts from these replicates. Particles ranging in size from 40-100 nm were considered exosomes.