To evaluate the viable LAB count, 10 g of sample was homogenized with 90 mL of saline (9 g L−1 NaCl solution). Serial dilutions of 10−4 to 10−8 with saline were used for sample preparation. Sterile MRS agar (5 mm thick; CM0361, Oxoid, Hampshire, UK) was used for bacterial growth on Petri dishes. The dishes were separately seeded with the sample suspension using surface sowing and were incubated under anaerobic conditions at 30 °C for 72 h. All results were expressed in log10 CFU mL−1 (colony-forming units per mL of sample) as the mean and standard deviation of three determinations.
Sterile mrs agar
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
Sterile MRS agar is a culture medium used for the isolation and enumeration of lactic acid bacteria. It provides the necessary nutrients and growth factors required for the cultivation of Lactobacillus and other lactic acid-producing microorganisms.
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Lab products found in correlation
2 protocols using sterile mrs agar
1
Viable Lactic Acid Bacteria Enumeration
2
Comprehensive Microbiological Analyses of Bacterial Cellulose
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Microbiological analyses (LAB, aerobic mesophilic spore-forming bacteria, the total number of enterobacteria, Escherichia coli, and fungi/yeast counts) were performed with BC samples that were ultrasonicated, fermented or not fermented, and before or after drying. For the evaluation of LAB count, a BC sample (10 g) was homogenized with 90 mL of NaCl solution (9 g/L). Serial dilutions of 10 -4 to 10 -8 with NaCl solution were used for sample preparation. Sterile MRS agar (CM0361, Oxoid) was used for bacterial growth on petri plates. The plates after seeding with the sample suspension were incubated under anaerobic conditions at 30°C for 72 h. The number of aerobic mesophilic spore-forming bacteria was determined on plate count agar (CM0325, Oxoid). MacConkey and Tryptone Bile X-glucuronide agar (both from Oxoid) were used for the determination of the total number of enterobacteria and Escherichia coli count (at 35-37°C for 18-24 h). The fungi and yeasts were determined in chloramphenicol agar (CM0549, Oxoid) after incubation at 25°C for 5 d. The number of microorganisms was calculated and expressed as log 10 of colony-forming units per gram or milliliters. All of the results were expressed as the mean values of 5 determinations.
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