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Hiv p24 rd1 fh190 1 1

Manufactured by Beckman Coulter
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The HIV-p24-RD1 (FH190-1-1) is a laboratory equipment product developed by Beckman Coulter. It is designed for the detection and quantification of the HIV-p24 antigen. The product utilizes a fluorescent-labeled antibody technology to measure the presence and concentration of the HIV-p24 antigen in biological samples.

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2 protocols using hiv p24 rd1 fh190 1 1

1

Polychromatic Flow Cytometry Analysis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The following fluorochrome-conjugated Abs were used for polychromatic flow cytometry analysis: CD3-Pacific-blue (UCHT1), CD4-Alexa-Fluor 700 (RPA-T4), CCR4-PE-Cy7 (1G1), CCR6-PE (11A9), CCR7-PE-Cy7 (3D12), CXCR3-PE-Cy5 (1C6) (BD Biosciences, San Diego, CA), CCR7-FITC (150503) (R&D Systems, Minneapolis, MN), CD45RA-APC-eFluor780 (HI100) (eBioscience, San Diego, CA), CD3-Alexa700 (UCHT1), CD326-BV650 (9C4), CD8-PerCP-Cy5.5 (RPA-T8), CD19-PerCP-Cy5.5 (HIB19), CD66b-PerCP-Cy5.5 (G10F5), HLADR-BV785 (L243; BioLegend), CD4-FITC (SFCI12T4D11) and HIV-p24-RD1 (FH190-1-1; Beckman Coulter, Fullerton, CA). Live/Dead Fixable Aqua Dead Cell Stain Kit (Vivid; Life Technologies, Burlington, ON) was used to exclude dead cells. Cells were analyzed by FACS using the BD-LSRII cytometer and BD-Diva (BD Biosciences, San Jose, CA), and FlowJo (©Tree Star, Inc., Ashland, OR) softwares. All Abs were titrated for an optimal noise/signal ratio and Ab cocktails were validated by comparing single to multiple staining. Positive gates were placed based on fluorescence minus one (FMO) controls [41 (link), 69 ]
Gosselin A., Wiche Salinas T.R., Planas D., Wacleche V.S., Zhang Y., Fromentin R., Chomont N., Cohen É.A., Shacklett B., Mehraj V., Ghali M.P., Routy J.P, & Ancuta P. (2016). HIV persists in CCR6+CD4+ T cells from colon and blood during antiretroviral therapy. AIDS (London, England), 31(1), 35-48.
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2

Polychromatic Flow Cytometry of Immune Markers

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The following fluorochrome-conjugated antibodies (Abs) were used for polychromatic flow cytometry analysis: CD3-Pacific-blue (UCHT1), CD4-Alexa-Fluor 700 (RPA-T4), CCR4-PE-Cy7 (1G1), CCR6-PE (11A9), CCR7-PE-Cy7 (3D12), CXCR3-PE-Cy5 (1C6) (BD Biosciences, San Diego, California, USA), CCR7-FITC (150503) (R&D Systems, Minneapolis, Minnesota, USA), CD45RA-APC-eFluor780 (HI100) (eBioscience, San Diego, California, USA), CD3-Alexa700 (UCHT1), CD326-BV650 (9C4), CD8-PerCP-Cy5.5 (RPA-T8), CD19-PerCP-Cy5.5 (HIB19), CD66b-PerCP-Cy5.5 (G10F5), human leukocyte antigen - antigen D related (HLA-DR)-BV785 (L243; Biolegend ,San Diego, California USA), CD4-FITC (SFCI12T4D11), and HIV-p24-RD1 (FH190–1–1); (Beckman Coulter, Fullerton, California, USA). Live/Dead Fixable Aqua Dead Cell Stain Kit (Vivid; Life Technologies, Burlington, Ontario, Canada) was used to exclude dead cells. Cells were analyzed by fluorescence-activated cell sorting (FACS) using the BD-LSRII cytometer and BD-Diva (BD Biosciences) and FlowJo (Tree Star, Inc., Ashland, Oregon, USA) softwares. All Abs were titrated for an optimal noise/signal ratio and Ab cocktails were validated by comparing single with multiple staining. Positive gates were placed based on fluorescence minus one controls [41 (link),69 ].
Gosselin A., Wiche Salinas T.R., Planas D., Wacleche V.S., Zhang Y., Fromentin R., Chomont N., Cohen É.A., Shacklett B., Mehraj V., Ghali M.P., Routy J.P, & Ancuta P. (2016). HIV persists in CCR6+CD4+ T cells from colon and blood during antiretroviral therapy. AIDS (London, England), 31(1), 35-48.
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