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Anti spike mab 1a9

Manufactured by GeneTex
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The Anti-spike mAb (1A9) is a monoclonal antibody that specifically binds to the spike protein of SARS-CoV-2. This product can be used for research purposes to study the interaction between the SARS-CoV-2 spike protein and potential target molecules.

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Lab products found in correlation

Luminescent substrate, by Merck Group (2 mentions) Hrp conjugated goat anti mouse secondary antibody, by Merck Group (2 mentions) Anti gapdh mab, by Thermo Fisher Scientific (2 mentions) Ibright cl1000, by Merck Group (1 mentions)

Close spelling variants of this product

Anti-SARS-CoV-2 Spike mouse mAb (1A9, (2 citations)

2 protocols using anti spike mab 1a9

1

SARS-CoV-2 Spike Protein Analysis

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Spike proteins were analyzed on immunoblots probed with transfected cells that were lysed in buffer containing 50 mM HEPES, 150 mM KCl, 2 mM EDTA, 0.5% NP-40, and protease inhibitor cocktail. Protein (40 μg) was separated by SDS-PAGE. The proteins were transferred to polyvinylidene difluoride membranes and probed with anti-spike mAb (1A9) (GeneTex), anti-p24 mAb (AG3.0) and anti-GAPDH mAb (Life Technologies) followed by goat anti-mouse HRP-conjugated secondary antibody (Sigma). The proteins were visualized by luminescent substrate (Millipore) on iBright CL1000.
Tada T., Zhou H., Dcosta B.M., Samanovic M.I., Mulligan M.J, & Landau N.R. (2021). Partial resistance of SARS-CoV-2 Delta variants to vaccine-elicited antibodies and convalescent sera. iScience, 24(11), 103341.
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2

Spike Protein and p24 Detection

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Cells were lysed in buffer containing 50 mM HEPES, 150 mM KCl, 2 mM EDTA, 0.5% NP-40, and protease inhibitor cocktail. Protein (40 μg) was separated by SDS-PAGE. The proteins were transferred to polyvinylidene difluoride membranes and probed with anti-spike mAb (1A9) (GeneTex), anti-p24 mAb (AG3.0) and anti-GAPDH mAb (Life Technologies) followed by goat anti-mouse HRP-conjugated secondary antibody (Sigma). The proteins were visualized by luminescent substrate (Millipore) on iBright CL1000.
Tada T., Zhou H., Samanovic M.I., Dcosta B.M., Cornelius A., Herati R.S., Mulligan M.J, & Landau N.R. (2022). Neutralization of SARS-CoV-2 Variants by mRNA and Adenoviral Vector Vaccine-Elicited Antibodies. Frontiers in Immunology, 13, 797589.
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