Images were captured on a Leica inverted TCS SP8 X confocal or DM2500 epifluorescence microscope. For time-lapse image capture, embryos were imaged as they developed in sea water-filled chambers on coverslip-bottom petri dishes (MatTek). Confocal image stacks were processed in Leica Application Suite or ImageJ. Video annotations were made using Camtasia software (TechSmith). 3D slices and projections were generated using Imaris (Bitplane) or Volocity (PerkinElmer) software. Kaede∷nls47 (link) was photoconverted as previously described48 (link). Neurite lengths and Golgi apparatus positioning were measured using ImageJ. Not all cells, neurites, and/or Golgi were visible in every embryo. Golgi positioning relative to BTN nuclei was measured in degrees of angle formed between a line traced anteriorly from the nucleus and another line traced through the middle of the Golgi complex. Thus, when the Golgi complex is perfectly aligned anterior to the nucleus, the angle is 0°, whereas if the Golgi complex is perfectly posterior to the nucleus, the angle is 180°. Rose plots (angle histograms) were generated in Matlab (http://www.mathworks.com/help/matlab/ref/rose.html ).
Inverted tcs sp8 x confocal
Manufactured by Leica
The Inverted TCS SP8 X confocal is a versatile microscopy system designed for advanced imaging applications. It features a high-resolution optical system, a flexible configuration, and powerful software control. The system allows for confocal imaging with excellent contrast and detail, enabling users to visualize and analyze complex biological samples.
Automatically generated - may contain errors
2 protocols using inverted tcs sp8 x confocal
1
Quantifying Neurodevelopmental Changes via Confocal Imaging
2
Quantifying Neurodevelopmental Changes via Confocal Imaging
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Images were captured on a Leica inverted TCS SP8 X confocal or DM2500 epifluorescence microscope. For time-lapse image capture, embryos were imaged as they developed in sea water-filled chambers on coverslip-bottom petri dishes (MatTek). Confocal image stacks were processed in Leica Application Suite or ImageJ. Video annotations were made using Camtasia software (TechSmith). 3D slices and projections were generated using Imaris (Bitplane) or Volocity (PerkinElmer) software. Kaede∷nls47 (link) was photoconverted as previously described48 (link). Neurite lengths and Golgi apparatus positioning were measured using ImageJ. Not all cells, neurites, and/or Golgi were visible in every embryo. Golgi positioning relative to BTN nuclei was measured in degrees of angle formed between a line traced anteriorly from the nucleus and another line traced through the middle of the Golgi complex. Thus, when the Golgi complex is perfectly aligned anterior to the nucleus, the angle is 0°, whereas if the Golgi complex is perfectly posterior to the nucleus, the angle is 180°. Rose plots (angle histograms) were generated in Matlab (http://www.mathworks.com/help/matlab/ref/rose.html ).
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