Anti trio h 120

The anti-Trio (H-120, 1:500), anti-Notch1 ICD (C-20-R, 1:1000), anti-Notch1 ECD (H-131, 1:500), anti-VE-cadherin (F-8, 1:200), and anti-VE-PTP (H-300, 1:250) antibodies were from Santa Cruz Biotechnology. Anti-VE-cadherin (ab33168, 1:2,000), anti-RFP (ab124754, 1:1000), and anti-β tubulin (ab6046, 1:5,000) antibodies were from Abcam. Anti-VEGFR2 antibody (55B11, 1:1000), anti-Notch1 V1754G (D3B8, 1:1000 WB), anti-HA (6E2, 1:1000 WB, AF647 conjugate IF) was from Cell Signaling Technologies. Anti-Rac1 antibody (102, 1:1,000) was from BD Biosciences. Rhodamine and Alexa Fluor 488-labelled phalloidin, Alexa Fluor-488, 568 and 647 goat anti-mouse and anti-rabbit IgG secondary antibodies were from Life Technologies. HRP-conjugated donkey anti-mouse, anti-rabbit, and anti-goat IgG secondary antibodies were from Fitzgerald. Anti-PTPRF antibody (SAB4200321, 1:1000), DAPI, DAPT, Evans Blue dye, dynasore hydrate, and formamide were from Sigma. Rac1 inhibitor NSC 23766 was from Santa Cruz Biotechnology.