Ab50247

Manufactured by Abcam

Ab50247 is a lab equipment product. It is a device used for laboratory analysis and research purposes.

Automatically generated - may contain errors

Lab products found in correlation

Ab66495, by Abcam (1 mentions) Affinipure goat anti human igg, by Jackson ImmunoResearch (1 mentions) Normal human igg control, by R&D Systems (1 mentions) Goat serum, by Merck Group (1 mentions) Ab128086, by Abcam (1 mentions) Ab128874, by Abcam (1 mentions) Cd11b, by Abcam (1 mentions) Flag tag (flag), by Merck Group (1 mentions) Mms 101r, by Fortrea (1 mentions) β actin, by Merck Group (1 mentions) Ab75476, by Abcam (1 mentions)

2 protocols using ab50247

Analyzing Autoantibody Reactivity in Rheumatoid Arthritis

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Samples were dotted onto NCMs at 10 ng/dot and blocked in 5% goat serum (Sigma-Aldrich) in TBS. Blots were incubated in either samples (RA SF or PB serum, NL PB serum, 1:10,000), normal human IgG control (R&D Systems, #1001A, 1 μg/mL), or control antibodies, including monoclonal mouse anti-ID1 (Abcam, #ab66495, clone 2456C1a, 1 μg/mL) and polyclonal rabbit anti-PAD4 (Abcam, #ab50247, 1 μg/mL), then probed with peroxidase AffiniPure goat anti-human IgG (Jackson ImmunoResearch, #109035003, 1:5,000). To verify specificity for citID1 reactivity of RA specimens, additional control blots were included on the same NCMs using various proteins, including noncitID1, BSA, citBSA, ENA-78, citENA-78 and rhPAD4. Densitometry analysis was performed using ImageJ.

Ohara R.A., Edhayan G., Rasmussen S.M., Isozaki T., Remmer H.A., Lanigan T.M., Campbell P.L., Urquhart A.G., Lawton J.N., Chung K.C., Fox D.A, & Ruth J.H. (2019). Citrullinated Inhibitor of DNA Binding 1 Is a Novel Autoantigen in Rheumatoid Arthritis. Arthritis & Rheumatology (Hoboken, N.j.), 71(8), 1241-1251.

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Protein Interactions and Modifications Assessment

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PAD4 (ab128086 and ab50247, Abcam), HA (MMS-101R, Covance), Flag (F3165, Sigma), AMC antibody kit (17-347, Millipore), E2F-1 (KH95 and C20, Santa Cruz Biotechnology), BRD4 (ab128874, Abcam), β-actin (A2228, Sigma), pRB (IF8, Santa Cruz Biotechnology), CD11B (ab75476, Abcam), mouse and rabbit secondary antibodies (GE Healthcare), and HA or Flag antibody-coupled agarose beads (Sigma) were used.

Ghari F., Quirke A.M., Munro S., Kawalkowska J., Picaud S., McGouran J., Subramanian V., Muth A., Williams R., Kessler B., Thompson P.R., Fillipakopoulos P., Knapp S., Venables P.J, & La Thangue N.B. (2016). Citrullination-acetylation interplay guides E2F-1 activity during the inflammatory response. Science Advances, 2(2), e1501257.

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