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3t4 macs microbeads positive column purification

Manufactured by Miltenyi Biotec
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The 3T4) MACS MicroBeads are a positive-column purification tool for cell separation. They are designed to enable the isolation and enrichment of specific cell populations from complex samples.

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MACS columns (296 citations) MACS microbeads (116 citations) MACS-column purification (6 citations) MACS purification (6 citations) Column purification (4 citations)

2 protocols using 3t4 macs microbeads positive column purification

1

Isolation of Naive CD4+ T Cells

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Naive splenocytes and peripheral lymph nodes (LNs) were isolated from 8–12-wk-old distal lck-Cre ROSA HuRfl/fl or ROSA HuRfl/fl littermate control mice. CD4+ T cells were isolated using murine anti-CD4 (L3T4) MACS MicroBeads positive-column purification, following the manufacturer’s protocol (Miltenyi Biotec). Naive CD4+ T cells were isolated from peripheral LNs and spleens (SPs) of mice using a MACS Mouse Naive CD4+ T Cell Isolation Kit, following the manufacturer’s protocol.
Techasintana P., Ellis J.S., Glascock J., Gubin M.M., Ridenhour S.E., Magee J.D., Hart M.L., Yao P., Zhou H., Whitney M.S., Franklin C.L., Martindale J.L., Gorospe M., Davis W.J., Fox P.L., Li X, & Atasoy U. (2017). The RNA-Binding Protein HuR Posttranscriptionally Regulates IL-2 Homeostasis and CD4+ Th2 Differentiation. ImmunoHorizons, 1(6), 109-123.
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2

Isolation of Naive CD4+ T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Naive splenocytes and peripheral lymph nodes (LNs) were isolated from 8–12-wk-old distal lck-Cre ROSA HuRfl/fl or ROSA HuRfl/fl littermate control mice. CD4+ T cells were isolated using murine anti-CD4 (L3T4) MACS MicroBeads positive-column purification, following the manufacturer’s protocol (Miltenyi Biotec). Naive CD4+ T cells were isolated from peripheral LNs and spleens (SPs) of mice using a MACS Mouse Naive CD4+ T Cell Isolation Kit, following the manufacturer’s protocol.
Techasintana P., Ellis J.S., Glascock J., Gubin M.M., Ridenhour S.E., Magee J.D., Hart M.L., Yao P., Zhou H., Whitney M.S., Franklin C.L., Martindale J.L., Gorospe M., Davis W.J., Fox P.L., Li X, & Atasoy U. (2017). The RNA-Binding Protein HuR Posttranscriptionally Regulates IL-2 Homeostasis and CD4+ Th2 Differentiation. ImmunoHorizons, 1(6), 109-123.
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