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2 protocols using lactose

1

Quantitative Analysis of Galactooligosaccharides

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All chemicals and analytical
standards of d-glucose, d-galactose, and lactose
used in this study
were purchased from Sigma-Aldrich (St. Louis, MO) unless stated otherwise
and were of the highest purity available. Vivinal GOS (V-GOS; 59%
GOS, 21% lactose, 19% glucose, and 1% galactose) and the GOS mixtures,
GOS I, GOS III, and GOS V are the commercial GOS standards from previous
work.23 (link) Isopropyl-β-d-thiogalactopyranoside
(IPTG) was obtained from Roth (Karlsruhe, Germany). Peptide pheromone
IP-673 was synthesized by Caslo (Lyngby, Denmark). The Glucose (HK)
Assay Kit for the determination of d-glucose was obtained
from Megazyme (Wicklow, Ireland).
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2

Comprehensive Carbohydrate Compound Sourcing

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Buffers were prepared or diluted with ddH2O, if not otherwise indicated. d(+)-galactose was purchased from AppliChem GmbH (Darmstadt, Germany). α-d-(+)-glucose, d-(+)-melibiose monohydrate, N-acetylneuraminic acid, β-d-gentiobiose, and lactose were purchased from Carl Roth GmbH + Co. KG (Karlsruhe, Germany). N,N′-diacetylchitobiose was purchased from Carbosynth Limited (Berkshire, UK). d-sorbitol, d-(+)-xylose, l-rhamnose monohydrate, and N-acetyl-d-galactosamine were purchased from Merck KGaA (Darmstadt, Germany). 1,4-β-d-galactobiose, galactan, and laminaribiose were purchased from Megazyme (Bray, Ireland). Sucrose and dithiothreitol (DTT) were purchased from Fisher Chemical (Schwerte, Germany). d-(+)-maltose monohydrate was purchased from TCI Deutschland GmbH (Eschborn, Germany). d-(+)-mannose was purchased from Thermo Fisher Scientific GmbH.—Acros Organics (Schwerte, Germany). N-acetyl-β-d-glucosamin was purchased from GLYCON Biochemicals GmbH (Luckenwalde, Germany). 2α-mannobiose was purchased from Dextra Laboratories Ltd. (Reading, UK).
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