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Alexa fluor 647 anti rabbit igg

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Alexa Fluor 647 anti-rabbit IgG is a secondary antibody conjugated with the Alexa Fluor 647 fluorescent dye. It is designed to detect and bind to rabbit immunoglobulin G (IgG) in various immunoassays and imaging applications.

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Lab products found in correlation

Ab56574, by Abcam (1 mentions) Ab6046, by Abcam (1 mentions) Sodium arsenite, by Merck Group (1 mentions) Rhodamine red x anti mouse igg, by Jackson ImmunoResearch (1 mentions) Prolong gold, by Thermo Fisher Scientific (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Anti vdac1, by Abcam (1 mentions) Intracellular staining permeabilization wash buffer, by BioLegend (1 mentions) Cytoflex flow cytometer, by Beckman Coulter (1 mentions) Alexa 488 conjugated anti chicken igy, by Thermo Fisher Scientific (1 mentions) Mms 435p, by Fortrea (1 mentions) Dneasy blood tissue kit, by Qiagen (1 mentions) Anti p ampk, by Cell Signaling Technology (1 mentions) Anti gabarap atg8a, by Cell Signaling Technology (1 mentions) Anti lamp1, by Abcam (1 mentions) Anti atp5a, by Abcam (1 mentions) Peroxidase affinipure donkey anti mouse igg, by Jackson ImmunoResearch (1 mentions) Anti mouse igg alexa fluor 488, by Jackson ImmunoResearch (1 mentions) Anti mouse igg dylight 405, by Jackson ImmunoResearch (1 mentions)

Close spelling variants of this product

Alexa Fluor 647 (152 citations) Alexa 647 (67 citations) Alexa Fluor 647 donkey anti-rabbit IgG (12 citations) Alexa Fluor 647 anti-rabbit (9 citations) Alexa Fluor 647 conjugated goat anti-rabbit IgG (7 citations) Alexa Fluor 647-conjugated donkey anti-rabbit IgG (7 citations) Alexa Fluor® 647 AffiniPure Donkey Anti-Rabbit IgG (H + L) (7 citations) Alexa Fluor 647-anti-rabbit IgG (H+L) (3 citations) Alexa Fluor 647 IgG (2 citations) Anti-Rabbit-Alexa 647 (2 citations)

6 protocols using alexa fluor 647 anti rabbit igg

1

Stress Granule Formation in U2OS Cells

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After specified durations of treatment with 0.5 mM sodium arsenite (Sigma-Aldrich) to induce stress granule formation5 (link), wild-type U2OS cells were fixed with 4% formaldehyde for 15 min, permeabilized with 0.1% Triton X-100 in phosphate-buffered saline (PBS) and blocked with 5 mg ml–1 bovine serum albumin (Sigma-Aldrich), in some cases also containing 0.25% Triton X-100. Fixed cells were incubated overnight at 4 °C with primary antibodies (mouse anti-G3BP (1:500, abcam ab56574) and rabbit anti-β-tubulin (1:200, abcam ab6046)) to stain the stress granules and microtubules. Note that G3BP does not co-precipitate in β-tubulin immunoprecipitation and is commonly used as a stress granule marker26 (link),65 (link). Secondary antibodies consisted of rhodamine Red-X anti-mouse IgG (1:500, Jackson ImmunoResearch) and Alexa Fluor 647 anti-rabbit IgG (1:500, Jackson ImmunoResearch). DNA was stained by incubating in 4′,6-diamidino-2-phenylindole (DAPI) (1:500, Sigma) for 15 min at room temperature. Coverslips were mounted in ProLong Gold (ThermoFisher).
Böddeker T.J., Rosowski K.A., Berchtold D., Emmanouilidis L., Han Y., Allain F.H., Style R.W., Pelkmans L, & Dufresne E.R. (2022). Non-specific adhesive forces between filaments and membraneless organelles. Nature Physics, 18(5), 571-578.
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2

Quantifying Mitochondrial Mass by VDAC-1 Staining

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Mitochondrial mass was evaluated using anti-VDAC-1 staining quantified by flow cytometry. Briefly, cells were collected and washed with cold phosphate buffer saline (PBS), fixed with 4% PFH, and kept on FACS buffer (PBS, 0.5% BSA, 0.05% Sodium Azide). For staining cells were washed twice with Intracellular Staining Permeabilization Wash Buffer (Biolegend, Catalogue No. 421002), and re-suspended in same buffer with anti-VDAC-1 (1:200, abcam, Catalogue No. 15895) for 30 min at room temperature. Cell samples were washed with wash buffer and incubated secondary (Alexa Fluor® 647 Anti-Rabbit IgG Jackson Immuno Research Laboratories, Catalogue No. 111-605-003) for 30 at room temperature in the dark. After two washes with FACS buffer, cells were re-suspended on FACS buffer and assessed on CytoFLEX flow cytometer (Beckman Coulter, Life Science, Indianapolis, IN, USA). Flow data analysis was performed using FlowJo software.
Tintos-Hernández J.A., Santana A., Keller K.N, & Ortiz-González X.R. (2021). Lysosomal dysfunction impairs mitochondrial quality control and is associated with neurodegeneration in TBCK encephaloneuronopathy. Brain Communications, 3(4), fcab215.
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3

Tracing iPSCs Differentiation in vivo

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NMR iPSCs or EGFP-expressed iPSCs grown on Matrigel were collected by 0.05% trypsin treatment and injected under the kidney capsules into non-obese diabetic (NOD)-SCID (NOD.CB17-Prkdc/J) mice. The kidney capsules were collected 10 weeks after the injection and processed for paraffin embedding and H&E staining or immunofluorescence co-staining with primary polyclonal antibodies against GFP (chicken IgY; Invitrogen; A10262) and TUJ-1(1:500, Covance; MMS-435P). Secondary antibodies were Alexa 488-conjugated anti-chicken IgY (Invitrogen; A-11039) and Alexa Fluor 647 anti-rabbit IgG (1:200 dilution, Jackson ImmunoResearch; 111-607-003). Genomic DNA of kidney capsules was extracted using a DNeasy Blood & Tissue Kit (QIAGEN). PCR with GoTaq polymerase using specific primers to M2rtTA lentiviral vector was performed to confirm the origin of NMR iPSCs in the kidney capsules.
Lee S.G., Mikhalchenko A.E., Yim S.H., Lobanov A.V., Park J.K., Choi K.H., Bronson R.T., Lee C.K., Park T.J, & Gladyshev V.N. (2017). Naked Mole Rat Induced Pluripotent Stem Cells and Their Contribution to Interspecific Chimera. Stem Cell Reports, 9(5), 1706-1720.
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4

Immunoblotting for Autophagy Markers

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The primary antibodies used were the anti‐Ubiquitin (Santa Cruz Biotechnology, Inc., sc‐8017), anti‐foxo (COSMO BIO CO, CAC‐THU‐A‐DFOXO), anti‐26S proteasome α (Santa Cruz Biotechnology, Inc., sc‐65,755), anti‐26S Proteasome p54 (Rpn10) (Santa Cruz Biotechnology, Inc., sc‐65,746), anti‐pAMPK (Cell Signaling Technology, #2535), anti‐GABARAP (Atg8a) (Cell Signaling Technology, #13733), anti‐Lamp1 (Abcam, ab30687), anti‐ATP5A (Abcam, ab14748), anti‐Actin (Cell Signaling Technology, #8457), and anti‐Gapdh (Sigma‐Aldrich, G9545). The anti‐ref(2)P/p62 antibody was kindly donated from Prof. G. Juhász. The secondary antibodies Peroxidase AffiniPure Donkey anti‐Mouse IgG (715‐035‐150) and Peroxidase AffiniPure Donkey anti‐Rabbit IgG (711‐035‐152) were purchased from Jackson ImmunoResearch Laboratories, Inc. The anti‐Rabbit‐IgG Alexa Fluor 647 (711‐605‐152), anti‐Rabbit‐IgG Alexa Fluor 488 (111‐545‐003), anti‐Mouse‐IgG Rhodamine (TRITC) AffiniPure (715‐025‐151), anti‐Mouse‐IgG Alexa Fluor 488 (115‐545‐003), and anti‐Mouse IgG DyLight™ 405 (715‐475‐151) antibodies were also from Jackson ImmunoResearch Laboratories, Inc. Ponceau S solution (6226–79) was from Sigma‐Aldrich.
Papanagnou E., Gumeni S., Sklirou A.D., Rafeletou A., Terpos E., Keklikoglou K., Kastritis E., Stamatelopoulos K., Sykiotis G.P., Dimopoulos M.A, & Trougakos I.P. (2022). Autophagy activation can partially rescue proteasome dysfunction‐mediated cardiac toxicity. Aging Cell, 21(11), e13715.
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5

Immunofluorescent Labeling of Lung Cells

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The following antibodies were used for immunofluorescent labeling: anti-claudin-3 (Life Technologies), anti-rabbit IgG-FITC (Vector Laboratories, Burlingame, CA), anti-occludin-AlexaFluor488 (Life Technologies), anti-histone H2A/H4 (BWA3), anti-mouse IgG-TRITC (Jackson Immunoresearch), anti-mouse Ly6G-AlexaFluor647 (eBioscience, San Diego, CA), anti-mouse CD11c-AlexaFluor488 (eBioscience), anti-mouse surfactant A (Millipore), anti-rabbit IgG-AlexaFluor647 (Jackson Immunoresearch). Slides were mounted with ProLong Gold anti-fade reagent containing DAPI (Life Technologies). Digital monochromatic images were acquired on a Nikon A-1 confocal system with Nikon Elements software and pseudocolored. For quantitative analysis of histone-associated cells, greater than 200 histone-associated cells were analyzed per lung (n=3 mice) for cell type-specific labeling (neutrophil, macrophage, or type II alveolar epithelial cells). The percentage of histone-associated cells that were also labeled for the cell type-specific marker is displayed.
Grailer J.J., Canning B.A., Kalbitz M., Haggadone M.D., Dhond R.M., Andjelkovic A.V., Zetoune F.S, & Ward P.A. (2014). Critical role for the NLRP3 inflammasome during acute lung injury. Journal of immunology (Baltimore, Md. : 1950), 192(12), 5974-5983.
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6

Sorting DDR2- and FSP-1-expressing Cells

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Indirect flow cytometry was done to sorting DDR2- and FSP-1-expressing cells isolated from the periosteum. Cells were incubated with the primary antibody DDR2 and FSP-1 for 30 min at 4 °C, followed by staining with fluorescent conjugated secondary antibodies Anti-Rabbit IgG Alexa Fluor 647 (Jackson, Philadelphia, PA, USA) and anti-Rat IgG DyLight 488 (Invitrogen, USA). The cells subjected to flow cytometry without the addition of a primary antibody or secondary antibody served as controls.
Yang H., Sun L., Cai W., Gu J., Xu D., Deb A, & Duan J. (2020). DDR2, a discoidin domain receptor, is a marker of periosteal osteoblast and osteoblast progenitors. Journal of bone and mineral metabolism, 38(5), 670-677.
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