Truseq srna kit

For flower buds of litchi, total RNA was extracted using TRIzol (Thermo Fisher Scientific) plus Fruit-mate (Takara) for polysaccharide/polyphenol removal, both according to the manufacturers’ instructions. For sRNA sequencing, 10 μg of the mixed total RNAs with RNA integrity number (RIN) ≥ 7.5 were used for sRNA library construction. sRNA libraries were constructed and sequenced on Illumina HiSeq 2500 platform at RIBOBIO (Guangzhou, China).
RNA of strawberry flower buds was extracted using PureLink Plant RNA Reagent. sRNA libraries were constructed using the Illumina TruSeq sRNA kit, and sequenced on the Illumina HiSeq platform at the University of Delaware.

Total RNA was extracted from the yellow and green parts of the B03S leaves using RNAiso Plus Total RNA Extraction Reagent (Takara, Beijing, China) following the manufacturer’s instructions, and the RNA integrity was checked via an Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). The qualified total RNA was further purified by an RNeasy Micro Kit (QIAGEN, GmBH, Hamburg, Germany) in conjunction with RNase-Free DNase (QIAGEN, GmBH, Hamburg, Germany).
RNA sequencing and analysis were conducted by staff at SHBIO (Biotechnology, Shanghai, China) and via its online platform (http://www.shbio.com (accessed on 1 May 2022)). The mRNA library was prepared from 1 μg of total RNA via a TruSeq sRNA Kit (Illumina, San Diego, CA, USA) and a cBot Clonal Amplification System (Illumina, San Diego, CA, USA). mRNA sequencing was performed on an Illumina HiSeq 2500 (Illumina, San Diego, CA, USA), with three biological replicates.

Total RNA extraction was performed using the mirVana miRNA isolation kit following the manufacturer’s protocol (Invitrogen, Waltham, MA). The RNA quality was analyzed using a 2100 Bioanalyzer instrument (Agilent Technologies, Santa Clara, CA), and RNA concentration was quantified using a Qubit fluorometer (Life Technologies, Carlsbad, CA). For sRNA sequencing, the total RNA was sent for library preparation and paired-end sRNA sequencing at the National Genomics Infrastructure (NGI) Stockholm, Sweden. The sRNA library was generated using a TruSeq sRNA kit (Illumina, San Diego, CA) and sequenced on one NovaSeq SP flow cell with 2 × 50-bp reads using Illumina NovaSeq 6000 equipment at NGI Stockholm. The Bcl to FASTQ conversion was performed using bcl2fastq v.2.19.1.403 from the CASAVA software suite. The quality scale used is Sanger/phred33/Illumina v.1.8+.