PGP (purity ≥98%) and PD (purity ≥98%) were purchased from Desite Biotechnology Co. Ltd. (Chengdu, China). The ambroxol hydrochloride oral solution was purchased from Sunflower Pharmaceutical Group Co., Ltd. (Hengshui, China). Rabbit anti-rat antibodies specific to interferon-γ (IFN-γ, bs-0480R), interleukin-4 (IL-4, bs-0581R), mucin 2 (MUC2, bsm-60016R), endothelin-1 (ET-1, bs-0954R), inducible nitric oxide synthase (iNOS, bs-2072R), T-bet (bs-3599R), GATA-3 (bs-1452R), polymer detection system kit (PV-6000), 3, 3′-diaminobenzidine (DAB), and horseradish peroxidase (HRP) were purchased from Beijing Biosynthesis Biotechnology Co., Ltd. (Beijing, China). Enzyme-linked immunosorbent assay (ELISA) kits for IFN-γ and IL-4 were purchased from Abcam (Cambridge, UK). The Alcian blue/periodic acid-Schiff (AB-PAS) stain kit and nitric oxide (NO) analysis kit were purchased from Beijing Solarbio Science &Technology Co., Ltd. (Beijing, China). Antibodies of FITC anti-rat CD4 (201,505), Alexa Fluor® 647 anti-rat IFN-γ (507,809), and PE anti-rat IL-4 (511,905) were purchased from BioLegend (California, United States). All other chemicals and solvents were of analytical grade or higher and were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).
Alcian blue periodic acid schiff ab pas stain kit
Manufactured by Solarbio
Sourced in China
The Alcian blue/periodic acid-Schiff (AB-PAS) stain kit is a laboratory reagent used for the combined staining of acidic and neutral mucopolysaccharides in tissue sections. The kit provides the necessary components to perform this histochemical staining procedure.
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Lab products found in correlation
2 protocols using alcian blue periodic acid schiff ab pas stain kit
1
Immunohistochemical Analysis of Airway Inflammation
2
Evaluating AMP-ZnONPs Efficacy Against H9N2 Virus
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AMP-ZnONPs was prepared according to the protocol described in our previous study (Bo et al., 2022 ). Chicken immunoglobulin G (IgG ) ELISA kit was purchased from Shanghai Enzyme-linked Biotechnology Co., Ltd. Goat Anti-Chicken IgA H&L (HRP ) was obtained from Abcam. The Hifair Ⅲ 1st Strand cDNA Synthesis SuperMix for qPCR was the product of Yeasen Biotechnology Co., Ltd. SYBR qPCR SuperMix Plus was purchased from Novoprotein. Total RNA extraction reagent was purchased from Vazyme Biotechnology Co., Ltd. TMB was purchased from Beyotime Biotechnology. Alcian Blue Periodic Acid Schiff (AB-PAS ) Stain Kit was purchased from Beijing Solarbio Science & Technology Co., Ltd. Avian influenza (Subtype H9) inactivated vaccine was purchased from Zhaofeng Hua Biotechnology (Nanjing) Co., ltd. Elisa plate was purchased from Suzhou cellpro biotechnology Co., ltd.
H9N2 virus (A/chicken/XZ/508/2019, EID50/0.1 mL= 10−8.83) was donated from the Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University.
H9N2 virus (A/chicken/XZ/508/2019, EID50/0.1 mL= 10−8.83) was donated from the Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University.
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