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Pbs tablets p4417

Manufactured by Merck Group
Sourced in United Kingdom

PBS tablets (P4417) are a laboratory product manufactured by Merck Group. The tablets contain the necessary components to prepare phosphate-buffered saline (PBS) solution when dissolved in water. PBS is a widely used buffer solution that maintains a stable pH and is commonly employed in various biological and biochemical applications.

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3 protocols using pbs tablets p4417

1

Polycaprolactone and Fibrinogen Hydrogel Preparation

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Polycaprolactone (PCL) (440744), fibrinogen
(FIB) (F8630), phosphate-buffered saline (PBS) tablets (P4417), fetal
bovine serum (FBS) (F7524), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic
acid (HEPES), and trypan blue (TP154) were purchased from Sigma. Acetic
acid was supplied from Merck. Chitosan (85/300/A1) was obtained from
Biolog. Dulbecco’s modified Eagle’s medium (DMEM), trypsin/EDTA
solution, and penicillin–streptomycin were obtained from Pan
Biotech. Cell-counting kit-8 from Dojindo Molecular Technologies,
4′,6-diamidino-2- phenylindole dihydrochloride (DAPI; 100 nM;
≥97%) from Santa Cruz Biotechnology, Inc., and the phalloidin
CruzFluor 488 conjugate were used.
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2

Phosphate Buffered Saline for Biomolecular Analysis

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Phosphate buffered saline (PBS. 0.01 M phosphate buffer, 0.0027 M potassium chloride, 0.137 M sodium chloride) was used for analysis. PBS tablets (P4417) were purchased from Sigma-Aldrich, UK. The control study proteins used were γ-globulin from human blood (G4386), albumin from human serum (A9511) and fibrinogen from human plasma (F3879), all sourced from Sigma Aldrich, UK. Streptavidin surface modified SPBs were purchased from Ademtech, France (03121), with a known mean diameter of 125 nm and a measured concentration of 1 × 10 12 mL -1 and used in all experiments. Invitrogen dynabeads were used, mean size of 1.05 μm.
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3

Phosphate Buffered Saline Preparation

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The initial buffer used for particle analysis was phosphate buffered saline (1× PBS tablet (0.01 M phosphate buffer, 0.0027 M potassium chloride, 0.137 M sodium chloride) in 200 mL deionised water (18.2 MΩ cm)). PBS tablets (P4417) were purchased from Sigma-Aldrich, UK.
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